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circRNA markers, kits and gene chips for AD screening

A gene chip and kit technology, applied in the field of circRNA markers and gene chips, can solve the problems of patients missing the intervention period

Active Publication Date: 2021-09-10
PEOPLES HOSPITAL OF XINJIANG UYGUR AUTONOMOUS REGION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the problem that existing AD diagnostic methods are difficult to screen in the absence of onset and early onset, resulting in a large number of patients missing the best intervention period, the present invention provides a circRNA marker and kit for AD screening And gene chips, to achieve the purpose of early detection and early prevention of AD so as to delay the progress of the disease

Method used

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  • circRNA markers, kits and gene chips for AD screening

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Comparison scheme
Effect test

Embodiment 1

[0025]Example 1: circRNA markers for AD screening

[0026] The present invention provides a circRNA marker for AD screening. The marker includes a combination of six circRNAs: hsa_circRNA_0054102, hsa_circRNA_0062028, hsa_circRNA_0001929, hsa_circRNA_0023262, hsa_circRNA_0090857 and hsa_circRNA_0092223.

Embodiment 2

[0027] Example 2: Kits for AD Screening

[0028] The invention provides a kit for AD screening, which includes: Taq enzyme, reverse transcriptase, buffer, dNTPs, MgCl 2 , DEPC water, negative control substance, positive control substance and primers and probes for hsa_circRNA_0054102, hsa_circRNA_0062028, hsa_circRNA_0001929, hsa_circRNA_0023262, hsa_circRNA_0090857 and hsa_circRNA_0092223.

[0029] The forward primer of hsa_circRNA_0054102 is shown in SEQ ID NO.1, the reverse primer of hsa_circRNA_0054102 is shown in SEQ ID NO.2; the forward primer of hsa_circRNA_0062028 is shown in SEQ ID NO.3, and the reverse primer of hsa_circRNA_0062028 As shown in SEQ ID NO.4; the forward primer of hsa_circRNA_0001929 is shown in SEQ ID NO.5, the reverse primer of hsa_circRNA_0001929 is shown in SEQ ID NO.6; the forward primer of hsa_circRNA_0023262 is shown in SEQ ID NO.7 , the reverse primer of hsa_circRNA_0023262 is shown in SEQ ID NO.8; the forward primer of hsa_circRNA_0090857 is s...

Embodiment 3

[0031] Example 3: Gene chip for AD screening

[0032] The invention provides a gene chip for AD screening, the gene chip includes a probe and a solid support, and the solid support includes a glass sheet, a silicon chip, a polypropylene membrane, a nitrocellulose membrane and a nylon membrane; The probe sequences are shown in SEQ ID NO.13-18.

[0033] The preparation method of the gene chip for AD screening specifically comprises the following steps:

[0034] (1) using six kinds of probes whose sequences are shown in SEQ ID NO.13~18, the average length of the nucleotides in this part is 60 bases; the 3' end of each probe is modified with amino group, so that It can covalently react with the polysilicon on the glass plate to fix it to the chip surface.

[0035] (2) These probes were respectively spotted three times in parallel on the glass plate used to prepare the chip. The diameter of each probe point was 150 μm, and the distance between the centers of adjacent probe points...

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Abstract

The present invention discloses circRNA markers, kits and gene chips for AD screening, wherein the markers include hsa_circRNA_0054102, hsa_circRNA_0062028, hsa_circRNA_0001929, hsa_circRNA_0023262, hsa_circRNA_0090857 and hsa_circRNA_0092223, which can detect the combination of six circRNA kits. The content of the above markers in peripheral blood; the circRNA markers, kits and gene chips for AD screening provided by the present invention have high stability, specificity and sensitivity, the sensitivity can reach 84.6%, and the specificity can reach 78.3% %, which has a high clinical reference value.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a circRNA marker, a kit and a gene chip for the diagnosis of Alzheimer's disease. Background technique [0002] Alzheimer's disease (Alzheimerdisease, AD), also known as senile dementia, is a degenerative disease of the central nervous system with an insidious onset and a chronic progressive course. It is the most common type of senile dementia. The main manifestations are neuropsychiatric symptoms such as progressive memory impairment, cognitive dysfunction, personality changes, and language barriers, which seriously affect social, occupational, and life functions. The etiology and pathogenesis of AD have not yet been elucidated, and the characteristic pathological changes are extracellular senile plaques formed by the deposition of β-amyloid protein, neurofibrillary tangles formed by hyperphosphorylation of tau protein, and neuron loss with glial cell proliferation Wait. The incide...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6883C12N15/11
CPCC12Q1/6883C12Q2600/158C12Q2600/178
Inventor 补娟史深赵宗锋翁振群牛晓珊王慧琴张晓玲张艳君张慧杨学磊依布拉音
Owner PEOPLES HOSPITAL OF XINJIANG UYGUR AUTONOMOUS REGION
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