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Licorice crude polysaccharide separating and purifying method

A technology for separation and purification of licorice crude polysaccharides, which is applied in the field of licorice crude polysaccharide extraction, can solve the problems of low purity of licorice polysaccharides, low membrane concentration efficiency, and high cost, and achieve the effects of improving resource utilization, improving process efficiency, and solving difficult processing

Inactive Publication Date: 2018-08-03
新疆乌拉尔甘草开发有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the prior art, Patent No.: CN 104644731 discloses a production process of licorice extract, which directly concentrates and packs the acid precipitation liquid, but the obtained licorice polysaccharide has low purity and deep color
Patent No.: CN 104479033 discloses a method for comprehensively separating and extracting glycyrrhizic acid, licorice flavonoids, and licorice polysaccharides from licorice. It uses the supernatant of acid precipitation as raw material, concentrates 10-50 times through membrane, and the obtained concentrated solution is sprayed Glycyrrhiza polysaccharide powder is obtained by drying, but this process does not remove impurities from the acid sedimentation liquid, and the membrane concentration efficiency is low and the cost is high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] A method for separating and purifying licorice crude polysaccharides, comprising the following steps:

[0026] 1. Take 1000mL of the acid precipitation waste liquid in the production process of glycyrrhizic acid, adjust the pH value to 6.8 with 20% sodium hydroxide solution, and pre-filter with the primary filter to obtain the primary filtrate for future use;

[0027] 2. Adsorb the above primary filtrate with the pretreated AB-8 macroporous resin, the amount of resin is 200mL, the flow rate of the sample is 1BV / h, and the post-column liquid is obtained;

[0028] 3. Use a high-efficiency filter membrane to filter the post-column liquid to obtain the final filtrate;

[0029] 4. Concentrate the final filtrate to about 150mL to obtain a concentrated solution. Add 4BV of 95% ethanol to the concentrated solution, stir overnight, and obtain a precipitate after filtration. The obtained precipitate is dried under reduced pressure to obtain a crude licorice polysaccharide. Use ph...

Embodiment 2

[0031] A method for separating and purifying licorice crude polysaccharides, comprising the following steps:

[0032] 1. Take 1000 mL of the acid precipitation waste liquid in the production process of glycyrrhizic acid, adjust the pH value to 6.5 with 20% sodium hydroxide solution, and pre-filter with the primary filter to obtain the primary filtrate, which is set aside;

[0033] 2. Adsorb the above primary filtrate with pretreated D101 macroporous resin, the amount of resin is 200mL, the sample loading flow rate is 1BV / h, and the post-column liquid is obtained;

[0034] 3. Use a high-efficiency filter membrane to filter the post-column liquid to obtain the final filtrate;

[0035] 4. Concentrate the final filtrate to about 160mL to obtain a concentrated solution. Add 4.5BV of 95% ethanol to the concentrated solution, stir, overnight, and obtain a precipitate after filtration. The obtained precipitate is dried under reduced pressure to obtain crude licorice polysaccharide. Us...

Embodiment 3

[0037] A method for separating and purifying licorice crude polysaccharides, comprising the following steps:

[0038] 1. Take 1000 mL of the acid precipitation waste liquid in the production process of glycyrrhizic acid, adjust the pH value to 7.0 with 20% sodium hydroxide solution, and pre-filter with the primary filter to obtain the primary filtrate, which is set aside;

[0039] 2. Adsorb the above primary filtrate with pretreated D101 macroporous resin, the amount of resin is 200mL, the sample loading flow rate is 1BV / h, and the post-column liquid is obtained;

[0040] 3. Use a high-efficiency filter membrane to filter the post-column liquid to obtain the final filtrate;

[0041] 4. Concentrate the final filtrate to about 155mL to obtain a concentrated solution. Add 4.5BV of 95% ethanol to the concentrated solution, stir, overnight, and obtain a precipitate after filtration. The obtained precipitate is dried under reduced pressure to obtain crude licorice polysaccharide. Us...

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PUM

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Abstract

The invention discloses a licorice crude polysaccharide separating and purifying method. The method comprises the following steps: 1) taking acid precipitation waste liquid during a glycyrrhizinic acid preparation method as a raw material, adjusting the waste liquid to neutrality, and employing a prefilter for prefiltration to obtain a primary filtrate; 2) absorbing and purifying the primary filtrate on a macroporous resin column to obtain elution liquor; 3) employing a high-efficiency filter membrane for filtering the elution liquor to obtain a final filtrate; and 4) concentrating the final filtrate, and performing alcohol precipitation, filtering and drying to obtain the licorice crude polysaccharide. The content of the licorice crude polysaccharide is higher than the content of the licorice crude polysaccharide obtained through water boiling and alcohol precipitation in the current traditional preparation technology, the acid precipitation waste liquid during the glycyrrhizinic acidpreparation method is taken as a raw material, the deposition influence of impurities such as protein during an alcohol precipitation process in a traditional technology can be effectively avoided, alot of technology steps for removing protein can be reduced, difficult processing problem of the acid precipitation waste liquid can be solved, technology efficiency is increased, and the resource utilization rate is increased.

Description

technical field [0001] The invention relates to the technical field of licorice crude polysaccharide extraction, in particular to a separation and purification method of licorice crude polysaccharide. Background technique [0002] Glycyrrhizae polysaccharide is one of the most effective parts of licorice research. It has anti-complement and mitosis-promoting activities, anti-tumor, anti-radiation, anti-virus, hypoglycemic and immune-regulating effects. Glycyrrhiza polysaccharides are extracted mostly from licorice extract as raw material , obtained by alcohol precipitation process. Then in industrial production, glycyrrhizin (also known as glycyrrhizic acid) in licorice is extracted as the main active ingredient, and the licorice residue or waste liquid after extracting glycyrrhizic acid causes environmental pollution and waste of resources. However, there are a large amount of licorice polysaccharides that can be extracted and utilized in licorice residue or waste liquid. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00B01D36/00
CPCB01D36/00C08B37/00
Inventor 周垠希谈满良
Owner 新疆乌拉尔甘草开发有限责任公司