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A tissue culture method for inducing differentiated adventitious buds by using Anthurium leaf veins

A technology for inducing differentiation and leaf veins, applied in the field of horticultural plant tissue culture, can solve the problems of reduced explant browning rate, reduced browning rate, and low callus induction rate, so as to reduce the risk of pollution and reduce inoculation Operation time, convenient and fast effect of inoculation operation

Active Publication Date: 2020-01-10
WUHAN ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The object of the present invention is to provide a kind of tissue culture method utilizing anthurium leaf veins to induce differentiated adventitious buds, which solves the problems of easy browning of explants and low callus induction rate in the process of anthurium callus induction culture, By using Anthurium leaf veins as explants, the browning rate of explants is greatly reduced during the culture process, the browning rate is reduced by 23.5-41.4%, the callus induction rate is greatly increased, and the induction rate is as high as 97.6%, thereby further increasing the number of offspring. The number of proliferation, the method is easy, the operation is simple, the explant browning rate is reduced, the callus induction rate is improved, and the anthurium tissue culture technology is improved

Method used

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  • A tissue culture method for inducing differentiated adventitious buds by using Anthurium leaf veins
  • A tissue culture method for inducing differentiated adventitious buds by using Anthurium leaf veins
  • A tissue culture method for inducing differentiated adventitious buds by using Anthurium leaf veins

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Embodiment 1

[0036] A method for tissue culture utilizing Anthurium leaf veins to induce differentiated adventitious buds, the steps of which are:

[0037] (1) Selection of female parent material: The potted anthurium variety "17-04", which grows robustly and has a beautiful plant shape, was selected as the female parent material from the base of Wuhan Weierfu Biotechnology Co., Ltd.

[0038] (2) Explant treatment: select the young young leaves and cut them off from the female parent plant, wash them with tap water for 20 minutes, and rub the surface of the leaves gently with your hands during this period. Soak in 75% (volume ratio) alcohol solution for 60 seconds, rinse with sterile water twice, soak in 10% (volume ratio) hydrogen peroxide solution for 15 minutes, and rinse with sterile water for 5 times on the ultra-clean workbench. The veins are cut from the blade along its edge, and the veins with a diameter greater than 1.5 mm are cut into 1 cm long sections for later use ( figure 1 ...

Embodiment 2

[0043] A method for tissue culture utilizing Anthurium leaf veins to induce differentiated adventitious buds, the steps of which are:

[0044] (1) Selection of female parent material: Anthurium "17-07", which is free from pests and diseases and has a beautiful plant shape, was selected as the female parent material from the base of Wuhan Weierfu Biotechnology Co., Ltd.

[0045](2) Explant treatment: select the young young leaves and cut them from the female parent, rinse them with tap water for 30 minutes, and rub the surface of the leaves gently with your hands during this period. Soak in 70% (volume ratio) alcohol solution for 50 seconds, rinse with sterile water three times, soak in 12% (volume ratio) hydrogen peroxide solution for 13 minutes, and rinse with sterile water four times on the ultra-clean workbench. Cut the leaf veins along its edge, and cut the leaf veins with a diameter greater than 1.5mm into 1cm long sections for later use ( figure 2 a).

[0046] (3) Ini...

Embodiment 3

[0050] A method for tissue culture utilizing Anthurium leaf veins to induce differentiated adventitious buds, the steps of which are:

[0051] (1) Selection of female parent material: The potted anthurium variety "17-04" or "17-07", which grows robustly and has a beautiful plant shape, was selected as the female parent material from the base of Wuhan Weierfu Biotechnology Co., Ltd.

[0052] (2) Explant treatment: select the young young leaves and cut them off from the female parent plant, wash them with tap water for 20 or 25 or 30 minutes, and gently rub the surface of the leaves with your hands during this period. Soak in 70 or 75% (volume ratio) alcohol solution for 30 or 40 or 50 or 60 seconds on the ultra-clean workbench, rinse with sterile water 2 or 3 times, and soak in 10 or 12% (volume ratio) hydrogen peroxide solution for 10 or 15 seconds. Or 20min, rinse with sterile water 3 or 4 or 5 times. The veins are cut from the blade along its edge, and the veins with a diam...

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Abstract

The invention discloses a tissue culture method for induced differentiation of adventitious buds by utilizing anthurium andraeanum veins. The tissue culture method comprises the following steps: firstly, selecting a female parent material; secondly, treating an explant: taking veins of female parent tender leaves as the tissue culture explant; washing the leaves with tap water after cutting from the female parent material; successively soaking with ethanol, flushing with sterile water, soaking with hydrogen peroxide and flushing with sterile water; cutting the veins along the edges of the veins; thirdly, starting culture: horizontally inoculating the cut veins into a starting medium and putting into a culture chamber for culturing; fourthly, carrying out differentiation culture on callus:after starting culture is ended, forming the callus at the edges of the veins and transferring the callus into a callus differentiation medium; fifthly, carrying out induced culture on the adventitious buds: after differentiation culture of the callus is ended, the callus is enlarged; transferring the callus into the medium for induced culture of the adventitious buds and inducing and differentiating the adventitious buds on the callus. The method is feasible and is easy to operate; the browning rate of the explant is reduced, the induction rate of the callus is improved and a tissue culture technology of the anthurium andraeanum is improved.

Description

technical field [0001] The invention belongs to the technical field of tissue culture of horticultural plants, and in particular relates to a tissue culture method for inducing and differentiating adventitious buds by using anthurium leaf veins, and the method is suitable for inducing differentiation of adventitious buds in the process of anthurium tissue culture. [0002] technical background [0003] Anthurium andraeanum, also known as anthurium or anthurium, is a tropical flower plant with high economic benefits. Its unique and beautiful flower shape, bright color and long flowering period make it a good flower decoration for various places. With the continuous improvement of people's living standards, the consumption of anthurium in the fields of home life, ceremonial exchanges, and event celebrations continues to increase, so that the market's demand for anthurium is also expanding. [0004] The progeny of the plants bred by tissue culture technology maintain the excell...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 王德欢施先锋葛米红梁欢周谟兵李爱成
Owner WUHAN ACADEMY OF AGRI SCI
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