Tissue culture method for induced differentiation of adventitious buds by utilizing anthurium andraeanum veins
A technology for inducing differentiation and leaf veins, which is applied in the field of horticultural plant tissue culture, can solve the problems of reduced explant browning rate, reduced browning rate, and low callus induction rate, so as to reduce the risk of pollution and reduce inoculation Operation time, convenient and fast effect of inoculation operation
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Embodiment 1
[0035] A method for tissue culture utilizing Anthurium leaf veins to induce differentiated adventitious buds, the steps of which are:
[0036] (1) Selection of female parent material: The potted anthurium variety "17-04", which grows robustly and has a beautiful plant shape, was selected as the female parent material from the base of Wuhan Weierfu Biotechnology Co., Ltd.
[0037] (2) Explant treatment: select the young young leaves and cut them off from the female parent plant, wash them with tap water for 20 minutes, and rub the surface of the leaves gently with your hands during this period. Soak in 75% (volume ratio) alcohol solution for 60 seconds, rinse with sterile water twice, soak in 10% (volume ratio) hydrogen peroxide solution for 15 minutes, and rinse with sterile water for 5 times on the ultra-clean workbench. The veins are cut from the blade along its edge, and the veins with a diameter greater than 1.5 mm are cut into 1 cm long sections for later use ( figure 1 ...
Embodiment 2
[0042] A method for tissue culture utilizing Anthurium leaf veins to induce differentiated adventitious buds, the steps of which are:
[0043] (1) Selection of female parent material: Anthurium "17-07", which is free from pests and diseases and has a beautiful plant shape, was selected as the female parent material from the base of Wuhan Weierfu Biotechnology Co., Ltd.
[0044](2) Explant treatment: select the young young leaves and cut them from the female parent, rinse them with tap water for 30 minutes, and rub the surface of the leaves gently with your hands during this period. Soak in 70% (volume ratio) alcohol solution for 50 seconds, rinse with sterile water three times, soak in 12% (volume ratio) hydrogen peroxide solution for 13 minutes, and rinse with sterile water four times on the ultra-clean workbench. Cut the leaf veins along its edge, and cut the leaf veins with a diameter greater than 1.5mm into 1cm long sections for later use ( figure 2 a).
[0045] (3) Ini...
Embodiment 3
[0049] A method for tissue culture utilizing Anthurium leaf veins to induce differentiated adventitious buds, the steps of which are:
[0050] (1) Selection of female parent material: The potted anthurium variety "17-04" or "17-07", which grows robustly and has a beautiful plant shape, was selected as the female parent material from the base of Wuhan Weierfu Biotechnology Co., Ltd.
[0051] (2) Explant treatment: select the young young leaves and cut them off from the female parent plant, wash them with tap water for 20 or 25 or 30 minutes, and gently rub the surface of the leaves with your hands during this period. Soak in 70 or 75% (volume ratio) alcohol solution for 30 or 40 or 50 or 60 seconds on the ultra-clean workbench, rinse with sterile water 2 or 3 times, and soak in 10 or 12% (volume ratio) hydrogen peroxide solution for 10 or 15 seconds. Or 20min, rinse with sterile water 3 or 4 or 5 times. The veins are cut from the blade along its edge, and the veins with a diam...
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