Reagent, kit and method for quickly detecting hydroxymethylfurfural in honey

Abstract

A reagent for quickly detecting hydroxymethylfurfural in honey comprises a reagent A and a reagent B, the reagent A is a hydrochloric acid solution, and the reagent B is a 2-thiobarbituric acid solution. A kit in the invention has the advantages of obvious color development and stable color when used to detect hydroxymethylfurfural, the absorbance of a 0-10 mg / kg hydroxymethylfurfural standard substance at different concentration gradients is linear, and the correlation coefficient R<2> reaches 0.9992 or more; and the method is fast, and allows an instrument to perform ten-channel detection, so only one second is taken during every detection, and the time needed by multiple samples is short.

Description

technical field [0001] The invention specifically relates to a rapid detection reagent, kit and detection method for hydroxymethylfurfural in honey, belonging to the technical field of food quality and safety detection. Background technique [0002] Hydroxymethylfurfural (HMF) is absent in fresh and pure honey. It is converted from fructose during storage or heating of honey, so it is one of the important signs to distinguish whether honey is fresh. According to international regulations, the content of hydroxymethylfurfural in honey should not exceed 40mg / kg, and the national standard GH / T 18796-2012 also stipulates that the content of hydroxymethylfurfural in honey should not exceed 40mg / kg. [0003] At present, the methods for determining the HMF content in honey mainly include liquid chromatography mass spectrometry, high performance liquid chromatography, gas chromatography, ultraviolet spectrophotometry, derivatization spectrophotometry and so on. Among them, liquid c...

AI Technical Summary

Problems solved by technology

Among them, liquid chromatography-mass spectrometry and high-performance liquid chromatography are more accurate, but the experimental steps are complicated, time-consuming, and require related large-scale, expensive and sophisticated equipment, which is not suitable for rapid on-site detection; and ultraviolet spectrophotometry is simple, but due to The instrument is more precise and expensive, and it is not suitable for rapid on-site detection

[0004] The commonly used on-site detection methods for hydroxymethylfurfural in food are derivatization spectrophotometry and colorimetry. Although the operation is simple, there are problems such as artificial judgment of color difference, poor accuracy, and low detection accuracy, which affect the accuracy of the results. It has been published In the rapid detection method of hydroxymethylfurfural in honey, its reagents and products have certain toxicity, and are not suitable for large-dose use in farmers' markets and supermarkets

[0005] In addition, although there are some corresponding detection reagents in the market, the color development effect is not good, and the linear correlation of color development is not high, and there is a problem of low detection accuracy in the rapid detection of instruments.