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A kind of fast propagation method of bellflower tissue culture

A technique for cultivating bellflower and tissue culture, which is applied in horticultural methods, botanical equipment and methods, plant regeneration, etc., can solve the problems that seedling propagation is limited by seasonality and is not ideal, and can improve the quality of seedlings and the reproduction coefficient. Effect

Active Publication Date: 2021-07-06
YANGTZE UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is only one comparative document on the research on the use of tissue culture technology to propagate A. chrysalis, that is, the patent application number is "CN201710170198.1", and the patent name is "a method for rapid propagation of A. chrysalis tissue culture", although it improves the seedling propagation speed , shortened the breeding cycle, and solved the problem of seasonal restrictions on seedling breeding, but its annual reproductive coefficient of aseptic buds is only 3.012, which is not ideal enough, and there is still room for improvement

Method used

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  • A kind of fast propagation method of bellflower tissue culture
  • A kind of fast propagation method of bellflower tissue culture
  • A kind of fast propagation method of bellflower tissue culture

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] (1) Selection and disinfection of explants: take the seeds of P. chinensis as explants, sterilize in 100 ml of 30% 84 disinfectant with 2-3 drops of Tween Tween20 added for 5-8min, rinse with sterile water 3-5 times, finally remove the surface moisture with sterilized filter paper to obtain explants, wherein the sterile water is distilled water sterilized by high pressure;

[0026] (2) Germination of explants to obtain sterile test-tube plantlets: Inoculate the explants obtained in step (1) into the basic medium, at a culture temperature of 23-27°C, with a light intensity of 1500 lux, and a light time of 8-10h / d Cultivate under the conditions of 30d to obtain aseptic test-tube plantlets, wherein basic medium is the agar that added 0.5mg / L gibberellin GA3, 2.0g / L activated carbon AC, 30g / L sucrose and 5g / L in MS medium , the pH value of the culture medium is 5.8;

[0027] (3) Test-tube plantlet clustering bud rapid propagation culture: the aseptic test-tube plantlet obt...

Embodiment 2

[0032] Different from Example 1, when the test-tube plantlets in the step (3) are rapidly propagated and cultivated in clusters, the propagation medium used is that 6-benzyl adenine 6-BA, 0.5 mg / L has been added in the WPM medium. 0.2mg / L kinetin KT, 0.5mg / L indole butyric acid IBA, 30g / L sucrose and 5g / L agar, the pH value of the medium is 5.8. Other steps are the same as in Example 1.

Embodiment 3

[0034] Different from Example 1, when the test-tube plantlets in the step (3) were rapidly propagated and cultivated in clusters of shoots, the propagation medium used was that 2.0mg / L of 6-benzyl adenine 6-BA, 0.5mg / L kinetin KT, 0.2mg / L indole butyric acid IBA, 30g / L sucrose and 5g / L agar, the pH value of the medium is 5.8. Other steps are the same as in Example 1.

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Abstract

The invention provides a method for rapid propagation of P. chinensis tissue culture, which specifically includes: (1) selection and disinfection of explants; (2) germination of explants to obtain aseptic test-tube plantlets; (3) rapid propagation and cultivation of test-tube plantlets Obtain clustered buds; (4) cultivate strong seedlings of clustered buds to obtain strong plants; (5) strong plant rooting culture to obtain complete rooted seedlings; (6) seedling hardening and transplanting; the propagation method and culture medium formula of the present invention carry out the cultivation of P. Tissue culture, the obtained single bud multiplication coefficient reaches 5-10 times, the rooting rate of the obtained tissue culture seedlings is above 95%, each plant has an average of 3-4 roots, the root length is 3-5 cm, and the transplanted sand bed survives The rate is above 98%; fast, convenient and efficient tissue culture of A. chrysalis, and large-scale production of A. chrysalis tissue-cultured seedlings are realized.

Description

technical field [0001] The invention relates to the technical field of asexual reproduction of seedlings, in particular to a method for rapid propagation of Suzuki chrysanthemum tissue culture. Background technique [0002] Tabebuia chrysantha is a deciduous tree belonging to the Bignoniaceae family, native to Mexico, Central America, and South America. Also known as Maofengling and Maohuangzhonghua, it is a small deciduous tree in the Bignoniaceae family. Campanula is a deciduous tree native to Mexico, Central America, and South America. It was introduced and planted in China from Paraguay in South America before 1997. Chrysanthemum chrysanthemum has obvious changes in four seasons, spring blossoms, summer fruits, autumn green, winter withering, giving colors to the seasons. In spring, there are clusters of campanula-shaped yellow flowers, which are the indicator flowers when spring comes; in summer, the sprouts are full of branches, followed by samara; in autumn, the bra...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 程玲
Owner YANGTZE UNIVERSITY