In-vivo cell capture device using functionalized protein threads as carrier

Abstract

The invention provides an in-vivo cell capture device using functionalized protein threads as a carrier. The in-vivo cell capture device comprises an in-vivo cell capture tube using the functionalizedprotein threads as the carrier; the in-vivo cell capture tube includes a fixing tube, a plurality of functionalized protein threads and a cell capture ligand fixed to the functionalized protein threads; one ends of the functionalized protein threads are fixed in the fixing tube; the fixing tube is sleeved with a guide pin; the guide pin is internally fixedly connected with a seal plug that is arranged around the fixing tube; the seal plug, the fixing tube and the guide pin form a chamber having one end opened in enclosed manner; the other ends of the functionalized protein threads are positioned in the chamber; the fixing tube is also connected with a guide screw; the guide screw is positioned in the guide pin and can relatively slide in the guide pin. The in-vivo cell capture device hasgood biocompatibility, and easily contacts target cells, so that in-vivo capturing efficiency of characteristic cells is improved.

Description

technical field [0001] The invention relates to the field of medical devices, in particular to an in vivo cell trap with functionalized protein silk as a carrier. Background technique [0002] The disease-related characteristic cells in human blood have important clinical application value, especially circulating tumor cells (CTC). However, the number of characteristic cells (especially CTC) in blood is extremely rare, and they are rare cells. Usually, the detection method includes the process of cell capture and enrichment. The capture method is divided into in vitro method and in vivo method. The former is prone to false negatives due to the small amount of blood in the test sample. It is for this reason that several in vivo cell capture methods are now developed. Whether in vivo or in vitro, the target cell-specific capture ligand (such as epithelial cell adhesion molecule antibody, nucleic acid aptamer, polypeptide aptamer) is usually immobilized on a carrier to constr...

AI Technical Summary

Problems solved by technology

However, in vivo cell traps constructed with these vectors have obvious deficiencies in four aspects

First, in order to resist the high-speed impact of venous blood, a larger diameter (above 500 microns) is often required to prevent the silk thread from being broken and placed in the blood vessel; second, the large-diameter material has enhanced rigidity and is compatible with the high-speed flow of target cells in flexible blood vessels. The probability of contact will be greatly reduced; third, under the premise of not affecting the normal function of blood vessels, it is impossible for large-diameter carrier materials to place traps containing multiple carrier wires (or tubes) in narrow veins. In fact, , are all traps constructed with a single wire (or tube) as the carrier material. The trap has a very small specific surface area, which will also greatly reduce the contact probability between the high-speed flowing target cells and the trap; the fourth Although these carrier materials have good biocompatibility and low toxicity, none of them can be biodegraded. Once broken and placed in blood vessels, it will definitely cause serious medical problems.

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