Application of PCSK9 inhibitor in malignant tumor immunotherapy
An inhibitor, tumor technology, used in anti-tumor drugs, blood/immune system cells, allergic diseases, etc., can solve problems such as anemia
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Embodiment 1
[0067] Example 1: Establishment of mouse tumor-bearing model
[0068]First, in the tumor-bearing experiment of mice in the PCSK9 antibody (purchased from BPS Bioscience) group, 20 male C57BL / 6J mice aged 6-8 weeks were selected, and each mouse was inoculated with 2×10 mice by subcutaneous injection. 6 A melanoma B16F10 cell, liver cancer cell Hepa1-6, lung cancer cell LLC, and prostate cancer cell RM-1 (purchased from the Chinese Academy of Sciences) were observed for tumor formation after 8 days. Sixteen mice with similar tumor sizes were selected, and then they were divided into a control group (8 mice) and a PCSK9 antibody group (8 mice).
[0069] Secondly, in the tumor-bearing experiment of mice in the PCSK9 antibody group, 30 male C57BL / 6J mice aged 6 to 8 weeks were selected, and each mouse was inoculated with 2×10 6 A melanoma B16F10 cell or a lung cancer cell LLC, and the tumor formation was observed after 8 days. Select 24 mice with similar tumor size, and then divi...
Embodiment 2
[0070] Example 2: Effects of PCSK9 inhibitors on tumor growth in mice
[0071] 1. Effect of PCSK9 antibody on tumor growth in mice
[0072] After the mice were grouped, the mice in the control group were given isotype control IgG, and the mice in the experimental group were given PCSK9 antibody (dissolved in saline), 10 mg / Kg, once every 3 days, intraperitoneal injection, or PD-1 antibody, every time 200μg (about 10mg / Kg) per mouse, once every 3 days, intraperitoneal injection, or a combination of the two. After the administration, the subcutaneous tumor size of mice in each group was measured every week, and the tumor volume was calculated (tumor volume=long diameter of tumor×short diameter of tumor) 2 / 2). The largest mouse tumor volume reached 2cm 3 When left and right, the mice in each group were sacrificed, the subcutaneous tumors were taken out, and the tumor growth curves were drawn.
[0073] Such as figure 1 , 2, 3 showed that the subcutaneous tumors in the contro...
Embodiment 3
[0080] Example 3: Detection of blood cholesterol-related indicators in mice
[0081] Before the mice in each group were sacrificed, the blood was collected with anticoagulant tubes, and the supernatant was collected after centrifugation to detect the total blood cholesterol content and low-density lipoprotein cholesterol content.
[0082] Such as Figure 5 As shown, the total blood cholesterol and low-density lipoprotein cholesterol levels in mice injected with PCSK9 antibody were significantly reduced, while triglyceride and high-density lipoprotein cholesterol had no significant changes, which was consistent with the medicinal effect of PCSK9 antibody.
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