Method for splitting and determining chiral pesticide quizalofop-ethyl and fluazifop-butyl enantiomers by super high performance combined chromatography-tandem mass spectrometry

Abstract

The invention belongs to the field of analytical chemistry and the technical field of pesticide residue detection, particularly discloses a method for splitting and determining chiral pesticide quizalofop-ethyl and fluazifop-butyl enantiomers by super high performance combined chromatography-tandem mass spectrometry, and relates to a splitting and quantifying method for enantiomers of chiral pesticides. According to the method, quizalofop-ethyl and fluazifop-butyl in tobacco and dried fruit are extracted by adopting a QuEChERS method; enantiomers of two chiral pesticides of the quizalofop-ethyl and the fluazifop-butyl are simultaneously detected by combining combined chromatography chiral stationary phase with triple quadrupole tandem mass spectrometry; the detection limits of the method are respectively 0.0018 and 0.0016 mg / kg. By the method, the quizalofop-ethyl and the fluazifop-butyl are chirally separated rapidly by adopting combined chromatography for the first time; the use of alarge number of organic solvents is reduced by taking supercritical CO2 as a moving phase, so that the method is green and environmentally friendly. The method provided by the invention is high in analysis speed by adopting the combined chromatography; the time consumption is only five minutes; the sensitivity is high; the separation degree between chiral isomers is high.

Description

technical field [0001] The invention belongs to the field of analytical chemistry and the technical field of pesticide residue detection, and specifically relates to a method for splitting and measuring the enantiomers of quizalofop-p-p and fluazifop-p-p-methyl in an ultra-high-efficiency convergence chromatography-tandem mass spectrometry technology, involving many A method for the resolution and quantification of the enantiomers of a chiral pesticide. Background technique [0002] 25% of the currently used pesticides are chiral, and there are enantiomeric differences in the biological activities of chiral pesticides, and their activity often exists in only one or a few enantiomers, while the enantiomers Dissolution and fate in the environment are often also significantly different. That is, there may be huge differences in the activity, toxicity, absorption, metabolism, and degradation of chiral pesticide enantiomers in the natural environment and organisms. [0003] Flu...

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Problems solved by technology

[Pesticides, 2012, 51(3), 193-196] used supercritical fluid chromatography to separate these two types of pesticides, but the analysis time was as long as 50 minutes, and the separation of fluazifop-methyl was not good

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