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The Application of Succinic Acid in Improving the Sensitivity of Bacteria to Antibiotics

A technology of antibiotics and succinic acid, which is applied in the field of medicine to achieve the effect of improving sensitivity, high safety and operability, and improving bactericidal effect

Active Publication Date: 2020-08-21
北京派胜医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no relevant report on whether succinic acid can increase the susceptibility of bacteria to antibiotics

Method used

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  • The Application of Succinic Acid in Improving the Sensitivity of Bacteria to Antibiotics
  • The Application of Succinic Acid in Improving the Sensitivity of Bacteria to Antibiotics
  • The Application of Succinic Acid in Improving the Sensitivity of Bacteria to Antibiotics

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Embodiment 1 succinic acid improves the sensitivity of clinical Escherichia coli to ampicillin

[0045] 1. Clinical drug resistance analysis of Escherichia coli

[0046] Escherichia coli (Escherichia coli, E.coli) is the most common bacterium in clinical practice. The 30 strains of Escherichia coli obtained from clinical practice were named as YT1-30. According to the NCCLS method, the minimum inhibitory concentration (MIC) of these 30 clinically obtained Escherichia coli to 8 antibiotics was detected, and Escherichia coli K12 was used as a control. The measurement results are shown in Table 1. From the results, it can be seen that the MIC of these 30 strains is more than 4 times higher than that of the control E.coli K12 (except that the MIC of YT1 to ceftazidime is the same as that of the control), that is, the 30 clinical Escherichia coli are all It is a multidrug-resistant bacteria, especially the MIC of ampicillin is 64-4000 times that of the control.

[0047] Tabl...

Embodiment 2

[0069] Example 2 Succinic acid improves the sensitivity of clinical Escherichia coli clinical drug-resistant bacteria to other antibiotics

[0070] 1. Improve sensitivity to penicillin antibiotics

[0071] After adding succinic acid to study whether clinical Escherichia coli has an effect on penicillin antibiotics, prepare two kinds of clinical Escherichia coli samples YT16 and YT28 according to step 2 of Example 1, add 20mM succinic acid and three kinds of penicillins respectively , After 4 hours of action, the number of viable bacteria was counted, and the survival rate was calculated.

[0072] The results showed that succinic acid could improve the sensitivity of clinical Escherichia coli YT16 and YT28 to these three antibiotics. Details are as follows:

[0073] For amoxicillin, after adding 20 mM succinic acid, adding 5 mg / mL amoxicillin can increase the sensitivity of YT16 and YT28 by 15.86 and 13.94 times respectively; adding 7.5 mg / mL amoxicillin can increase the sens...

Embodiment 3

[0079] Embodiment 3 succinic acid improves the sensitivity of various bacteria to ampicillin

[0080] Pick Escherichia coli, Vibrio alginolyticus, Vibrio parahaemolyticus, Edwardsiella tarda, Pseudomonas aeruginosa, Streptococcus beta and other bacterial clones into 100mL LB liquid medium, at 37°C or 30°C, Cultured at 200rpm for 16h to reach saturation. Collect 20 mL of each bacterial liquid, centrifuge at 8000 rpm for 5 min, remove the supernatant and wash the cells with an equal volume of 0.85% saline, and finally suspend the cells with 1×M9 basic liquid medium (containing 10 mM acetate) , adjust the OD value of the bacterial solution to 0.5, and then aliquot 5 mL into test tubes for later use.

[0081] The above-prepared bacterial solution was divided into two groups according to the type of bacteria: the control group (only ampicillin was added) and the experimental group (ampicillin and succinic acid were added). Add 20 mM succinic acid and ampicillin at 2 times the min...

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Abstract

The invention belongs to the technical field of medicine, and specifically discloses the application of succinic acid in improving the susceptibility of bacteria to antibiotics. The present invention finds that succinic acid increases the number of antibiotics entering the bacteria by increasing the proton kinetic potential of the bacteria, and finally kills the bacteria. Therefore, succinic acid can improve the sensitivity of bacteria to antibiotics, thereby overcoming the problem of bacterial drug resistance. Combining succinic acid with antibiotics can significantly improve the bactericidal effect of antibiotics, and compared with the existing antibacterial drugs that only use antibiotics, it has better effects, higher safety and operability.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to the application of succinic acid in improving the susceptibility of bacteria to antibiotics. Background technique [0002] Pathogenic bacteria seriously endanger human health and the sustainable development of the breeding industry, and effective measures need to be taken to prevent and control them. Since Fleming (Fleming) discovered penicillin in 1929, a large number of studies on various antibiotics have opened the era of human antibiotics, which play an important role in the treatment of infectious diseases, making the use of antibiotics increasingly widespread. Although the use of antibiotics can effectively prevent and control diseases, the overuse and misuse of antibiotics can lead to bacterial resistance. Resistant strains develop resistance to otherwise effective antibiotics, making infections difficult to control. Therefore, it is very important to adopt ...

Claims

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Application Information

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IPC IPC(8): A61K31/194A61K45/06A61K31/43A61P31/04
CPCA61K45/06A61P31/04A61K31/194A61K31/43A61K2300/00Y02A50/30
Inventor 李惠彭博彭宣宪
Owner 北京派胜医药科技有限公司
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