Method for extracting proteins from micro-algae residues
A technology of microalgae residue and protein, which is applied in the field of protein extraction, can solve the problems of lack of effective utilization technology of algae residue, and achieve the effect of improving the overall economy, reasonable process flow, and increasing economic benefits
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Embodiment 1
[0022] A method for extracting protein from microalgae residue, comprising the following steps:
[0023] 1) Alkaline extraction of algae residue protein: add 100 L of 1wt% sodium hydroxide solution to 750 g of chlorella algae residue after extracting algae pigment, stir and heat extraction at 30°C for 60 min, and place at 4°C after reaction Stand still for 3 h, discard the precipitate, and obtain the alkaline extract;
[0024] 2) Protein isoelectric point precipitation: adjust the pH of the alkaline extract obtained in step 1) to 3.5 with 4wt% sulfuric acid, and place it on a centrifuge at 6000 r / min for 6 minutes to obtain crude protein precipitation;
[0025] 3) Preparation of protein powder: after reconstitution of the crude protein precipitate obtained in step 2), desalination and concentration were carried out using an ultrafiltration membrane with a molecular weight cut-off of 3 kD, and then the concentrated solution was dried with hot air at 55°C for 24 hours to obtain ...
Embodiment 2
[0028] A method for extracting protein from microalgae residue, comprising the following steps:
[0029] 1) Alkaline extraction of protein from algae residue: add 100 L of 10wt% sodium hydroxide solution to 1000 g of Dunaliella algae residue after extraction of algae oil, stir and heat extraction at 45°C for 50 min, after reaction, put it on a centrifuge for 8000 r Centrifuge for 5 min at 1 / min, discard the precipitate, and obtain the alkaline extract;
[0030] 2) Protein isoelectric point precipitation: adjust the pH of the alkaline extract obtained in step 1) to 4.0 with 72wt% sulfuric acid, and filter with a 0.2 µm microfiltration membrane to obtain crude protein precipitation;
[0031] 3) Preparation of protein powder: After reconstitution of the crude protein precipitate obtained in step 2), use a nanofiltration membrane with a molecular weight cut-off of 250 Da for desalination and concentration. The concentrated solution is kept at a temperature of 60°C and a vacuum of ...
Embodiment 3
[0034] A method for extracting protein from microalgae residue, comprising the following steps:
[0035] 1) Alkaline extraction of protein from algae residue: add 100 L of 20wt% sodium hydroxide solution to 1250 g of Chlamydomonas algae residue after extracting algae pigment and oil, stir and heat extraction at 60°C for 25 min, and use 0.2 µm microfiltration after reaction Membrane filtration, discard the precipitate, and obtain the alkaline extract;
[0036] 2) Protein isoelectric point precipitation: adjust the pH of the alkaline extract obtained in step 1) to 4.5 with 6 mol / L hydrochloric acid, and place it on a centrifuge at 10,000 r / min for 3 minutes to obtain crude protein precipitation;
[0037]3) Preparation of protein powder: reconstitute the crude protein precipitate obtained in step 2), desalt and concentrate using an ultrafiltration tube with a molecular weight cut-off of 10 kD, and freeze-dry the concentrated solution for 24 hours to obtain protein powder;
[003...
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