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Method for extracting proteins and polysaccharides from microalgae residues synchronously

A technology of synchronous extraction and microalgae algal residue, applied in the field of synchronous extraction of protein and polysaccharide, can solve the problem of failing to realize the comprehensive utilization of multi-component or full-component of microalgae cells, achieve comprehensive utilization, increase economic benefits, Simple to use effects

Inactive Publication Date: 2018-08-17
FUZHOU UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are few reports on the high-value development and utilization of algae residues, and the comprehensive utilization of multi-components or all components of microalgae cells has not yet been realized.

Method used

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  • Method for extracting proteins and polysaccharides from microalgae residues synchronously

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Experimental program
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Effect test

Embodiment 1

[0025] 1) Simultaneous extraction of protein and polysaccharides from algal residues: Add 100 L of 5wt% sodium hydroxide solution to 375 g of chlorella algal residues after extracting algae oil, and heat and stir for 180 min at 30°C, 8000 rpm / min Centrifuge for 5 min under the conditions, discard the precipitate, and the supernatant is the alkaline extraction solution;

[0026] 2) Separation of algal residue protein and polysaccharide: add 300 L of absolute ethanol to the alkaline extract obtained in step 1), stir evenly, and then place it at 4°C for 2 h to obtain supernatant and precipitate respectively;

[0027] 3) Preparation of algae residue protein powder: Evaporate the supernatant obtained in step 2) under reduced pressure to remove the ethanol solvent, adjust the pH of the solution to neutral with concentrated hydrochloric acid, and then use a 250 Da nanofiltration membrane to concentrate the supernatant to its original volume Half of the concentrated solution is spray-...

Embodiment 2

[0031]1) Simultaneous extraction of protein and polysaccharides from algal residues: Add 100 L of 10wt% sodium hydroxide solution to 1125 g of Scenedesmus algal residues after extracting algae pigments, heat and stir for 70 min at 80°C, and then place at 4°C Stand still for 2 h under the same conditions, discard the precipitate, and the supernatant is the alkaline extraction solution;

[0032] 2) Separation of algal residue protein and polysaccharide: add 400 L of absolute ethanol to the alkaline extract obtained in step 1), stir evenly, and then centrifuge at 8000 rpm / min for 6 min to obtain supernatant and precipitate respectively;

[0033] 3) Preparation of algal residue protein powder: Evaporate the supernatant obtained in step 2) under reduced pressure to remove the ethanol solvent, adjust the pH of the solution to neutral with 6 mol / L hydrochloric acid, and then dialyze the supernatant for 48 h with a 3500 Da dialysis membrane , using a 250 Da nanofiltration membrane to ...

Embodiment 3

[0037] 1) Simultaneous extraction of protein and polysaccharides from algal residues: Add 100 L of 15wt% sodium hydroxide solution to 1500 g of Chlamydomonas algal residues after extracting algae pigments, heat and stir for 120 min at 75°C, and extract at 8000 rpm / min Centrifuge for 5 min, discard the precipitate, and the supernatant is the alkaline extraction solution.

[0038] 2) Separation of algal residue protein and polysaccharide: add 500 L of absolute ethanol to the alkaline extract obtained in step 1), stir evenly, and then centrifuge at 8000 rpm / min for 6 min to obtain supernatant and precipitate respectively;

[0039] 3) Preparation of algae residue protein powder: Evaporate the supernatant obtained in step 2) under reduced pressure to remove the ethanol solvent, adjust the pH of the solution to neutrality with 3 mol / L hydrochloric acid, and then use an ultrafiltration membrane with a molecular weight cut-off of 10 kDa to concentrate on the The volume of the clear li...

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Abstract

The invention discloses a method for extracting proteins and polysaccharides from microalgae residues synchronously. The method comprises the following steps: 1) adding a sodium hydroxide solution into algae residues, and performing stirring, heating, extraction and solid-liquid separation to obtain an alkali extracting solution; 2) adding absolute ethanol into the alkali extracting solution prepared in the step 1), uniformly stirring, and then performing solid-liquid separation to obtain supernatant and precipitate respectively; 3) evaporating the supernatant prepared in the step 2) under reduced pressure to remove an ethanol solvent, adjusting the PH of the solution to neutral with hydrochloric acid, and then performing desalination, concentration and drying in sequence to obtain proteinpowder; 4) washing and drying the precipitate prepared in the step 2) with the absolute ethanol to obtain polysaccharide powder. The method has reasonable process flow, and is easy to operate; wastealgae residues generated after extraction of pigments, grease and pigment grease are utilized fully, and the waste is turned into treasure, so that the economic benefit is increased, the industry chain of microalgae is extended, and the comprehensive utilization of microalgae cells is realized.

Description

technical field [0001] The invention relates to a method for synchronously extracting protein and polysaccharide from microalgae dregs, belonging to the field of biotechnology. Background technique [0002] Microalgae have the characteristics of high photosynthetic efficiency, fast growth rate, and strong adaptability to the environment. Their metabolism can produce various cell products such as oil, polysaccharide, pigment, and protein. Among them, algae oil can be applied to the preparation of biodiesel and other renewable energy sources. In particular, some microalgae oils also contain high levels of long-chain unsaturated fatty acids, such as eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), which can be developed Used as healthcare and pharmaceutical products (Chen C Y, Chen Y C, Huang H C, et al. Engineering strategies for enhancing the production of eicosapentaenoic acid (EPA) from an isolated microbial Nannochloropsis oceanica CY2[J]. Bioresource Technolo...

Claims

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Application Information

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IPC IPC(8): C07K1/14C08B37/00
CPCC07K1/145C08B37/0003
Inventor 谢友坪陈剑锋刘乐冕石新国沈英郑向南
Owner FUZHOU UNIVERSITY