Seaweed extract for cartilage repair and preparation method thereof
A technology of seaweed extract and cartilage repair, applied in medical preparations containing active ingredients, bone diseases, pharmaceutical formulas, etc., can solve the difficulties in the production, processing and popularization of seaweed extracts, high viscosity of seaweed extracts, and molecular weight of active substances major issues
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Embodiment 1
[0057] The preparation method of brown algae extract for cartilage repair comprises the following steps:
[0058] Step 1: Weigh 100g of brown algae powder, add 5 times the weight of brown algae powder in absolute ethanol, mix well, reflux and degrease in a water bath at 65°C for 30 minutes, filter with gauze, and take the filter residue; put the filter residue in a ventilated place to completely volatilize the ethanol, Obtain defatted brown algae powder;
[0059] Step 2: Weigh 30g of defatted brown algae powder, add boiled and cooled water to 90°C at a material ratio of 1:20 (g / mL), heat and reflux at 90°C for 2 hours, then naturally cool to 30°C, and turn at 4500 rpm Centrifuge for 10 minutes per minute to collect the supernatant A;
[0060] Step 3: Add 340 mL of cetyltrimethylammonium bromide aqueous solution with a concentration of 0.01 g / mL to the supernatant A obtained in step 2, keep stirring during the addition, and the speed is 100 rpm; , centrifuged at 7000 rpm for ...
Embodiment 2
[0063] The preparation method of brown algae extract for cartilage repair comprises the following steps:
[0064] Step 1: Weigh 100g of brown algae powder, add 5 times the weight of brown algae powder in absolute ethanol, mix well, reflux and degrease in a water bath at 65°C for 30 minutes, filter with gauze, and take the filter residue; put the filter residue in a ventilated place to completely volatilize the ethanol, Obtain defatted brown algae powder;
[0065] Step 2: Weigh 30g of defatted brown algae powder, add boiled and cooled water to 90°C at a material ratio of 1:20 (g / mL), heat and reflux at 90°C for 2 hours, then naturally cool to 30°C, and turn at 4500 rpm Centrifuge for 10 minutes per minute to collect the supernatant A;
[0066] Step 3: Add 40 mL of trichloroacetic acid solution with a concentration of 0.05 g / mL to the supernatant A obtained in Step 2, place it at 4°C for 12 hours, centrifuge at 4500 rpm for 20 minutes, and take supernatant B; The supernatant B...
Embodiment 3
[0070] The preparation method of brown algae extract for cartilage repair comprises the following steps:
[0071] Step 1: Weigh 100g of brown algae powder, add 5 times the weight of brown algae powder in absolute ethanol, mix well, reflux and degrease in a water bath at 65°C for 30 minutes, filter with gauze, and take the filter residue; put the filter residue in a ventilated place to completely volatilize the ethanol, Obtain defatted brown algae powder;
[0072] Step 2: Weigh 30g of defatted brown algae powder, add boiled and cooled water to 90°C at a material ratio of 1:20 (g / mL), heat and reflux at 90°C for 2 hours, then naturally cool to 30°C, and turn at 4500 rpm Centrifuge for 10 minutes per minute to collect the supernatant A;
[0073] Step 3: Add 40 mL of trichloroacetic acid solution with a concentration of 0.05 g / mL to the supernatant A obtained in Step 2, place it at 4°C for 12 hours, centrifuge at 4500 rpm for 20 minutes, and take supernatant B; The supernatant B...
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