Fluorescence excitation device of fluorescence detector

Abstract

The invention discloses a fluorescence excitation device of a fluorescence detector with advantages of simple structure and low manufacturing cost. The fluorescence excitation device of the fluorescence detector comprises a sealed opaque detection box body, wherein a light incident slit is formed in the detection box body, a first plane mirror for reflecting incident light is arranged in the lightincident slit in the detection box body in the direction of a light path, a concave grating is arranged on a light path of light reflected by the first plane mirror in the detection box body, a second plane reflector which reflects a part of light higher than the upper edge of a light inlet window of a flowing pool to a silicon photocell arranged in the detection box body is arranged on a light path of dispersed light of the concave grating in the detection box body, the flowing pool for placing to-be-detected objects is arranged on the light path of the dispersed light of the concave gratingin the detection box body, the light inlet window and a light outlet are formed in the flowing pool, the width of a light band after the concave grating is dispersed at the flowing pool is greater than that effective size of the light inlet window, a visible light concentrating lens is arranged in the light emergent direction of the flowing pool in the detection box body, and a light emergent slit is formed in the detection box body.

Description

technical field [0001] The invention relates to a fluorescence excitation device of a fluorescence detector. Background technique [0002] At present, the commonly used fluorescence detector uses xenon lamp as the excitation light source to excite the substance with fluorescent properties in the object to be tested to generate fluorescence, and then it is analyzed by a monochromator. Since the luminous intensity of the xenon lamp is unstable, and if the incident light intensity fluctuates during fluorescence excitation, the effect of fluorescence detection will be affected. Therefore, it is necessary to detect the intensity of incident light to ensure the reliability of the detection results of the fluorescence detector. [0003] The incident light intensity monitoring generally uses silicon photocells, which are sensitive to light intensity. If the irradiation is excessive, the silicon photocells will enter the non-linear working area, and even blind the silicon photocells....

AI Technical Summary

Problems solved by technology

After the beam splitter is installed in the current optical path, a part (generally 50%) of the monochromatic excitation light is separated, and the monochromatic light irradiated on the sample is weakened, which reduces the utilization rate of the light source energy and reduces the operating efficiency of the instrument. Measurement sensitivity

[0006] 2. The working wavelength of the instrument is in the range of 200nm to 800nm, the beam splitting ratio of the beam splitter varies greatly and is uneven

After the monochromatic light is focused by the condenser, the energy will be lost, which further reduces the utilization rate of the light source energy and further reduces the measurement sensitivity of the instrument.

Similarly, the price of ultraviolet condensers with working wavelengths in the range of 200nm to 800nm ​​is also more expensive

[0008] 4. This kind of structure is more complicated and the cost is high; it makes the debugging and calibration technology of the whole instrument complex

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