Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Cell elution method based on drug target residence time

A residence time, cell technology, applied in the field of cell elution, which can solve the problems of low screening throughput, inconvenient operation, and dependence on radioisotope labeling ligands.

Inactive Publication Date: 2018-08-21
XUZHOU MEDICAL UNIV
View PDF4 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to overcome the problems of inconvenient operation, low screening throughput, and dependence on radioactive isotope-labeled ligands in existing detection methods, the present invention provides a cell elution method based on the residence time of drug targets, which is used to screen cells with different drug targets. residence time compound

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Cell elution method based on drug target residence time

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0012] A cell elution method based on the residence time of the drug target, the method is: using highly expressed human adenosine A 1 CHO / ADORA1 stably transfected cells for the receptor screen for adenosine A with different residence times 1 Receptor agonists, the specific steps are as follows:

[0013] (1) CHO / ADORA1 cells were cultured and collected in vitro, divided into two equal parts, marked as group a and group b, each containing 4000 cells;

[0014] (2) Add 0.8U / mL adenosine deaminase to the DMEM / F12 medium as the reaction buffer for cell elution experiments; place the cells in group a in the prepared reaction buffer for cell elution experiments; add excess The compound to be tested was incubated at 37°C for 1 hour to allow the compound to be tested to interact with the adenosine A expressed on the cells 1 The receptors are fully bound; after incubation for 1 hour, transfer the above cell mixture to a centrifuge tube, centrifuge at 4°C for 3 minutes at a speed of 3...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a cell elution method based on drug target residence time. The method is characterized in that to-be-detected compounds are fully incubated with cells with drug targets expressed, the to-be-detected compounds dissociated from the drug targets are continuously removed by repeating the steps of elution, incubation and centrifugation three times, efficacy of the compounds notdissociated from the drug targets after elution is then detected by using a cell function experiment, an obtained result is compared with produced efficacy of to-be-detected compounds with the same concentration after full incubation with cells with drug targets expressed without elution, incubation and centrifugation, and residence time of the detected compounds is analyzed. The cell elution experiment method has the characteristics of being pollution-free, high in flux, simple to operate and the like.

Description

technical field [0001] The invention relates to a cell elution method, in particular to a cell elution method based on the residence time of a drug target. Background technique [0002] Drug target residence time is an emerging pharmacological concept in recent years. This molecular binding kinetics index reflects the existence time of the complex formed between the two after the drug binds to its target. Determining the molecular dynamics characteristics of drug-target binding is of great significance in the early drug design and screening process. Existing research results have shown that optimizing the residence time of the drug on the target can improve the clinical efficacy of the drug and reduce the side effects of the drug. Therefore, the screening and optimization of lead compounds based on the residence time of drug targets may provide a new basis for the design and discovery of innovative drugs, and the establishment of a fast and effective screening method based...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N33/50
CPCG01N33/5008
Inventor 郭栋云意刘春吉侯仲志印晓星
Owner XUZHOU MEDICAL UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com