Fingerprint spectrum of herba rabdosiae japonicae as well as establishment method and application of fingerprint spectrum

A technology of blue calyx incense tea vegetable and fingerprint spectrum is applied in the field of quality control of traditional Chinese medicinal materials to achieve the effects of monitoring quality, high sensitivity, good separation and identification

Active Publication Date: 2018-08-24
HANGZHOU HUQINGYUTANG PHARM CO LTD +1
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there is no report on the fingerprint and its establishment method of the blue calyx in the prior art. Therefore, it is of great significance to develop a fingerprint that can comprehensively evaluate the quality of the blue calyx

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Fingerprint spectrum of herba rabdosiae japonicae as well as establishment method and application of fingerprint spectrum
  • Fingerprint spectrum of herba rabdosiae japonicae as well as establishment method and application of fingerprint spectrum
  • Fingerprint spectrum of herba rabdosiae japonicae as well as establishment method and application of fingerprint spectrum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] The establishment of embodiment 1 fingerprint collection:

[0054]Take 1.0g of the powder of the above-ground part of the blue calyx fragrant tea, accurately weighed, accurately add 25mL of 70% methanol containing 0.2% formic acid, weigh the weight, ultrasonic for 30min, let cool, weigh, make up the lost weight with solvent, shake Evenly, filter, and get the continued filtrate to obtain the test solution;

[0055] (2) HPLC detection:

[0056] Draw above-mentioned step (1) gained need testing solution, inject in the high-performance liquid chromatograph and carry out high-performance liquid chromatography detection, obtain described fingerprint collection;

[0057] Wherein, the chromatographic detection conditions are as follows:

[0058] C18 chromatographic column (250mm×4.6mm, 5μm); methanol (A)-0.1% phosphoric acid (B) gradient elution, the elution program is shown in Table 1, and the running time is 55min; the volume flow rate is 1.0mL / min; the detection wavelength...

Embodiment 2

[0063] The comparison fingerprints of the blue calyx fragrant tea vegetables generated in Example 1 are used as a comparison, and the Chinese medicine chromatographic fingerprint similarity evaluation system software provided by the National Pharmacopoeia is used to measure the similarity of the fingerprints of 12 batches of blue calyx fragrant tea vegetables samples. see results figure 2 and Table 2. A total of 15 common peaks were detected in 12 batches of S. indigo, and six main components including rabdosiin, s. The similarity of 12 batches of blue calyx from different places of origin was between 0.874 and 0.990, with an average of 0.9556.

[0064] Table 2 The results of the similarity determination

[0065]

Embodiment 3

[0066] The investigation of embodiment 3 mobile phase solvent system

[0067] Kromasil 100-5C18 (250×4.6mm, 5μm) chromatographic column was selected. Methanol-0.1% phosphoric acid and acetonitrile-0.1% phosphoric acid were used as the mobile phase to test the influence of different solvent systems on the determination. It was found that the chromatogram of the acetonitrile-0.1% phosphoric acid system showed poor separation of phenolic acids and fewer peaks in the chromatogram. The separation of phenolic acid components in methanol-0.1% phosphoric acid system is better than that in acetonitrile-0.1% phosphoric acid system. Finally, methanol-0.1% phosphoric acid is used as the mobile phase for fingerprint determination, see image 3 .

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a fingerprint spectrum of herba rabdosiae japonicae as well as an establishment method and application of the fingerprint spectrum. The method comprises preparation of a test sample and high performance liquid chromatography detection conditions, wherein the high performance liquid chromatography detection conditions are as follows: a chromatographic column is a C18 chromatographic column and a mobile phase is a methanol-0.1 percent phosphoric acid solution; a gradient elution manner is adopted: the time is 0min, 30min and 55min, the amount of methanol is 20 percent, 50percent to 100 percent and the sample feeding amount is 3 to 10mu L; the detection wavelength is 232nm, the flow speed is 1.0mL / min and the column temprature is 20 DEG C to 35 DEG C. According to thefingerprint spectrum disclosed by the invention, an established herba rabdosiae japonicae medicinal material fingerprint spectrum determines 15 common peaks, and overall evaluation on medicinal material quality is facilitated and the uniformity and stability of intrinsic quality are ensured through abundant chromatographic peak information and evaluation of the similarity of the common peaks. Thefingerprint spectrum can be used for effectively monitoring the quality of a herba rabdosiae japonicae medicinal material and identifying the authenticity of the herba rabdosiae japonicae medicinal material.

Description

technical field [0001] The invention relates to the technical field of quality control of traditional Chinese medicinal materials, and in particular to a fingerprint of a calyx calyx and its establishment method and application. Background technique [0002] The blue calyx fragrant tea vegetable comes from Rabdosia japonica (Burm.f.) Haravar. glaucocalyx (Maxim.) Hara, which is mainly distributed in northern provinces such as Heilongjiang, Jilin, Liaoning, Shandong, Hebei and Shanxi, and is rich in resources. Its medicinal part is the dry aerial part. The diameter of the original plant stem is 3-8mm, the surface is gray-green to gray-brown, sparsely pubescent, the center of the section is pithed, not hollow. Leaves gray-green to gray-brown, ovate to broadly ovate when flattened, 5-13cm long, 3-7cm wide; margins obtusely serrated, sparsely pilose or pilose on both sides. Harvest in summer and autumn, remove impurities after harvest, and dry in the shade or in the sun. [0...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N30/02G01N30/86G01N30/74
CPCG01N30/02G01N30/74G01N30/8686
Inventor 陈炜祝明韩俊琦李文庭
Owner HANGZHOU HUQINGYUTANG PHARM CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap