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Application of sCD40L protein to preparation of kit for early diagnosis of aortic dissection

A technology for aortic dissection and diagnostic kits, which is applied in the fields of disease diagnosis, biological testing, material testing, etc., can solve problems such as the relationship between sCD40L proteins that are rarely reported in research, and achieve the effect of less trauma and effective diagnosis

Inactive Publication Date: 2018-08-24
GENERAL HOSPITAL OF THE NORTHERN WAR ZONE OF THE CHINESE PEOPLES LIBERATION ARMY
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AI Technical Summary

Problems solved by technology

[0004] Previous studies believed that sCD40L protein plays a key role in the occurrence and development of atherosclerosis, but few studies have reported the relationship between sCD40L protein and aortic dissection

Method used

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  • Application of sCD40L protein to preparation of kit for early diagnosis of aortic dissection
  • Application of sCD40L protein to preparation of kit for early diagnosis of aortic dissection
  • Application of sCD40L protein to preparation of kit for early diagnosis of aortic dissection

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Experimental program
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Effect test

Embodiment 1

[0043] Research basis of the present invention.

[0044] 1. Enzyme-linked immunosorbent assay was used to detect the expression changes of plasma sCD40L in patients with aortic dissection.

[0045] 1.1. Research object.

[0046] A total of 152 patients who were diagnosed with aortic disease by contrast-enhanced aortic CT from May 2015 to March 2017 in the Department of Cardiovascular Medicine, General Hospital of Shenyang Military Command due to chest pain, including 116 patients with aortic dissection, and aortic perforation There were 8 cases of permeable ulcer, 12 cases of pseudoaneurysm, 7 cases of acute aortic intramural hematoma, and 9 cases of aortic aneurysm. Grouping of patients with aortic dissection: The onset time is the most significant chest pain, the acute phase is within 7 days of onset, the subacute phase is 7 days to 14 days after onset (including 14 days), and the chronic phase is more than 14 days after onset.

[0047] 1.2 Experimental process.

[0048]1...

Embodiment 2

[0069] 2.1. Specimen collection and sample processing.

[0070] 2.1.1. Collection of specimens: 152 subjects were selected, and their venous blood was drawn immediately after admission, and 5ml of blood specimens were collected with vacuum anticoagulation tubes containing EDTA;

[0071] 2.1.2. Sample processing: within 10 minutes of drawing venous blood, centrifuge the anticoagulant tube at 3000 rpm for 10 minutes, separate the plasma, store the processed plasma in a -80°C refrigerator, and use a double-antibody sandwich enzyme-linked immunosorbent assay The concentration of plasma sCD40L was detected by ELISA method; blood samples were collected by procoagulant tubes and immediately sent to the Laboratory Department of our hospital to detect the concentrations of hs-CRP and D-dimer.

[0072] 2.2. Determination of sCD40L: using the detection kit of the present invention, its components are as follows: washing solution, diluent, standard, CD40L antibody, horseradish peroxidase-...

Embodiment 3

[0081] 3.1. Collection of normal human aorta and aortic specimens of patients with aortic dissection.

[0082] A young autopsy patient with no history of acute coronary syndrome or aortic disease was collected from the aorta of a young autopsy patient who died due to accident; aortic dissection specimens were obtained during aortic replacement in cardiovascular surgery. Significantly is the initial onset time, within 7 days of onset is the acute phase, 7 days to 14 days (including 14 days) is the subacute phase, and more than 14 days is the chronic phase; the control group is without acute coronary syndrome, major History of arterial disease, young autopsy aorta who died due to accident; all aortas were fixed in 10% paraformaldehyde; all selected samples were approved by the ethics committee of Shenyang Military General Hospital and signed informed consent.

[0083] 3.2. Preparation of paraffin sections.

[0084] Materials obtained: the tissues of patients with aortic dissect...

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Abstract

The invention belongs to the field of diagnosis kit molecular biology, and particularly relates to application of sCD40L protein to preparation of a kit for early diagnosis of aortic dissection. A diagnosis reagent uses sCD40L protein as a marker; the expression quantity of the sCD40L protein in plasma is detected; early diagnosis of risk of the aortic dissection occurrence is realized; the aorticdissection can be fast and effectively diagnosed; in addition, the characteristics of small trauma, simplicity and easy implementation are also realized.

Description

technical field [0001] The invention belongs to the field of molecular biology of diagnosis kits, and in particular relates to the application of sCD40L protein in the preparation of early diagnosis kits for aortic dissection. Background technique [0002] Aortic dissection is a dangerous disease with a high mortality rate. Imaging examinations such as aortic enhanced CT and magnetic resonance imaging (MRI) are currently the main methods for diagnosing aortic dissection, but the above methods have diagnostic delays and are expensive. , complex operation and other disadvantages, and all are invasive examinations, there is still a lack of specific biomarkers for the diagnosis of aortic dissection. Previous studies have confirmed some promising biomarkers for early diagnosis of aortic dissection, but their sensitivity and specificity are poor. Therefore, it is urgent to find some specific biological markers to quickly diagnose aortic dissection. [0003] CD40L is a transmembr...

Claims

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Application Information

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IPC IPC(8): G01N33/68
CPCG01N33/6893G01N2800/329
Inventor 王效增何瑞霞张效林张权宇
Owner GENERAL HOSPITAL OF THE NORTHERN WAR ZONE OF THE CHINESE PEOPLES LIBERATION ARMY
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