Application of sCD40L protein to preparation of kit for early diagnosis of aortic dissection

A technology for aortic dissection and diagnostic kits, which is applied in the fields of disease diagnosis, biological testing, material testing, etc., can solve problems such as the relationship between sCD40L proteins that are rarely reported in research, and achieve the effect of less trauma and effective diagnosis

Inactive Publication Date: 2018-08-24
GENERAL HOSPITAL OF THE NORTHERN WAR ZONE OF THE CHINESE PEOPLES LIBERATION ARMY
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  • Abstract
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Problems solved by technology

[0004] Previous studies believed that sCD40L protein plays a key role in the occurrence and development of

Method used

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  • Application of sCD40L protein to preparation of kit for early diagnosis of aortic dissection
  • Application of sCD40L protein to preparation of kit for early diagnosis of aortic dissection
  • Application of sCD40L protein to preparation of kit for early diagnosis of aortic dissection

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Experimental program
Comparison scheme
Effect test

Example Embodiment

[0042] Example 1

[0043] The research basis of the present invention.

[0044] 1. Enzyme-linked immunosorbent assay was used to detect the changes of plasma sCD40L expression in patients with aortic dissection.

[0045] 1.1. Research object.

[0046] A total of 152 patients who were diagnosed with aortic disease by aortic-enhanced CT from May 2015 to March 2017 due to chest pain in the Department of Cardiology of the General Hospital of Shenyang Military Region were consecutively selected, including 116 patients with aortic dissection and aortic puncture. There were 8 cases of permeable ulcer, 12 cases of pseudoaneurysm, 7 cases of acute aortic intramural hematoma, and 9 cases of aortic aneurysm. Grouping of patients with aortic dissection: the most significant chest pain is the initial time of onset, the acute phase is within 7 days of the onset, the subacute phase is 7 to 14 days (including 14 days), and the chronic phase is more than 14 days.

[0047] 1.2 Experimental process.

[0...

Example Embodiment

[0068] Example 2

[0069] 2.1. Collection of specimens and processing of samples.

[0070] 2.1.1. Specimen collection: 152 study subjects were selected, and their venous blood was drawn immediately after admission, and 5ml of blood specimens were collected with a vacuum anticoagulation tube containing EDTA;

[0071] 2.1.2. Sample processing: within 10 minutes of drawing venous blood, centrifuge at 3000 rpm in anticoagulation tube for 10 minutes, separate the plasma, store the processed plasma in the refrigerator at -80℃, adopt double antibody sandwich enzyme-linked immunoassay The concentration of sCD40L in plasma was detected by ELISA method; blood samples were collected from the coagulation tube and sent to the laboratory of our hospital for detection of hs-CRP and D-dimer concentrations.

[0072] 2.2. Determination of sCD40L: Using the detection kit of the present invention, its components are as follows: washing liquid, diluent, standard substance, CD40L antibody, horseradish pero...

Example Embodiment

[0080] Example 3.

[0081] 3.1. Collection of aortic specimens from normal human aorta and patients with aortic dissection.

[0082] Collected 1 case of aorta in a young autopsy without acute coronary syndrome or aortic disease, and died of accident; aortic dissection specimen was obtained during aortic replacement in cardiovascular surgery. The tissues of aortic dissection patients were divided into groups: chest pain was the most severe Significantly the time of onset of onset, within 7 days of onset is the acute stage, 7 to 14 days (including 14 days) of onset is the subacute stage, and more than 14 days is the chronic stage; the control group is no acute coronary syndrome, the main A history of arterial disease, the aorta of a young autopsy who died accidentally; all aortas were fixed in 10% paraformaldehyde; selected samples must be approved by the Ethics Committee of the General Hospital of Shenyang Military Region and signed an informed consent.

[0083] 3.2. Preparation of p...

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Abstract

The invention belongs to the field of diagnosis kit molecular biology, and particularly relates to application of sCD40L protein to preparation of a kit for early diagnosis of aortic dissection. A diagnosis reagent uses sCD40L protein as a marker; the expression quantity of the sCD40L protein in plasma is detected; early diagnosis of risk of the aortic dissection occurrence is realized; the aorticdissection can be fast and effectively diagnosed; in addition, the characteristics of small trauma, simplicity and easy implementation are also realized.

Description

technical field [0001] The invention belongs to the field of molecular biology of diagnosis kits, and in particular relates to the application of sCD40L protein in the preparation of early diagnosis kits for aortic dissection. Background technique [0002] Aortic dissection is a dangerous disease with a high mortality rate. Imaging examinations such as aortic enhanced CT and magnetic resonance imaging (MRI) are currently the main methods for diagnosing aortic dissection, but the above methods have diagnostic delays and are expensive. , complex operation and other disadvantages, and all are invasive examinations, there is still a lack of specific biomarkers for the diagnosis of aortic dissection. Previous studies have confirmed some promising biomarkers for early diagnosis of aortic dissection, but their sensitivity and specificity are poor. Therefore, it is urgent to find some specific biological markers to quickly diagnose aortic dissection. [0003] CD40L is a transmembr...

Claims

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Application Information

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IPC IPC(8): G01N33/68
CPCG01N33/6893G01N2800/329
Inventor 王效增何瑞霞张效林张权宇
Owner GENERAL HOSPITAL OF THE NORTHERN WAR ZONE OF THE CHINESE PEOPLES LIBERATION ARMY
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