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Steroid derivatives and their preservation solutions and applications

A technology of steroids and derivatives, which is applied in the preservation solution of steroids derivatives, steroids derivatives and its application fields, and can solve the problems of inaccurate test results, unstable concentration, and strong hydrophobicity of the kit , to achieve the effect of improving solubility and dispersion uniformity, solubilization, suspension and dispersion, and avoiding coagulation and sedimentation

Active Publication Date: 2021-04-20
SHENZHEN NEW INDS BIOMEDICAL ENG
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The purpose of the present invention is to overcome the above-mentioned deficiencies in the prior art, provide a kind of steroid derivatives and its application, to solve the steroid hormones used for steroid hormone detection which are highly hydrophobic, insoluble or difficult to dissolve in water Technical problems of inaccurate hormone detection
[0010] Another object of the present invention is to provide a preservation solution of steroid hormones to solve the problem that the actual concentration of steroid hormones is unstable due to the fact that steroid hormones are prone to coagulation and adsorption during the liquid preservation process of steroid hormones. technical problem
[0011] Another object of the present invention is to provide a detection reagent and a test kit for detecting steroid hormones, so as to solve the technical problems that existing detection reagents for detection of steroid hormones have unstable concentrations and inaccurate test results of the test kits

Method used

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  • Steroid derivatives and their preservation solutions and applications
  • Steroid derivatives and their preservation solutions and applications
  • Steroid derivatives and their preservation solutions and applications

Examples

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Effect test

Embodiment 11- Embodiment 17

[0067] Examples 11-17 respectively provide a steroid derivative. The structural formulas of the steroid derivatives in each of Examples 11-17 are shown in Table 1 below:

[0068] Table 1

[0069]

[0070]

[0071] 2. Preservation solution embodiment of steroid hormone

Embodiment 21- Embodiment 24 and comparative example 1

[0073] Examples 21-24 and Comparative Example 1 respectively provide a preservation solution of steroid hormones. The components contained in the preservation solutions of Examples 21-24 and Comparative Example 1 are shown in Table 2 below:

[0074] Table 2

[0075]

[0076]

[0077] 3. Detection reagent and kit embodiment for detecting steroid hormones

Embodiment 31- Embodiment 310 and comparative example 31- comparative example 36

[0079] The present embodiment 31-310 respectively provides a kind of kit for detecting steroid hormones, which comprises the following components:

[0080] (1) Magnetic microspheres coated with steroid hormone antibody, wherein the concentration of magnetic microspheres is 1 mg / mL, and the concentration of anti-steroid hormone antibody is 10 μg / mL;

[0081] (2) ABEI-labeled steroid hormone antibody, wherein the concentration of ABEI: 250ng / mL, the concentration of anti-steroid hormone antibody: 5000ng / mL;

[0082] (3) calibrator in embodiment 31-310 shown in table 3 and comparative example 31-36:

[0083] The low-concentration calibrator contains the steroid derivative antigens shown in Examples 11-17 in Table 1, and the steroid derivative antigens shown in Examples 11-17 are respectively dissolved in the in the preservation solution;

[0084] The high-concentration calibrator contains the steroid derivative antigens shown in Examples 11-17 in Table 1, and the steroid deriva...

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Abstract

The invention discloses a steroid derivative, its preservation solution and its application, and belongs to the technical field of in vitro diagnostic reagents. The steroid derivatives contain a cyclopentane polyhydrophenanthrene nucleus, and at least one carbon position of the cyclopentane polyhydrophenanthrene nucleus is substituted with a hydrophilic group. The steroid hormone preservation solution includes a buffer solution and a surfactant dissolved in the buffer solution. The application of the steroid derivatives and the preservation solution in the preparation of detection agents and kits for detecting steroid hormones. The steroid derivatives of the present invention have good hydrophilicity and solubility, can be uniformly and stably dispersed in a solution, and can be used as steroid hormone antigens to realize accurate detection of steroid hormones. The preservation solution of the steroid hormone can effectively improve the solubility and dispersion uniformity of the steroid derivatives, and avoid the adsorption of the steroid derivatives on the surface of the high molecular polymer.

Description

technical field [0001] The invention belongs to the technical field of in vitro diagnostic reagents, and in particular relates to a steroid derivative and its application, a preservation solution of the steroid derivative and the application of the preservation solution. Background technique [0002] Steroid hormones are an important class of endocrine hormones, which play a wide range of roles in maintaining the body's homeostasis and normal physiological activities, promoting the development of sexual organs, and maintaining reproductive system functions. Steroid hormone drugs can be divided into adrenal cortex hormones and sex hormones according to their pharmacological effects, the latter including androgens, estrogens, progestins and steroidal contraceptives. According to the chemical structure, it can be divided into androstanes, estrones and pregnanes, of which androgens belong to androstanes, estrogens belong to estrones, and adrenal cortex hormones, progestins and s...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/74G01N33/543G01N21/76C07J1/00C07J7/00
CPCC07J1/0025C07J1/0059C07J1/007C07J1/0085C07J7/007G01N21/763G01N33/54326G01N33/74G01N2333/575
Inventor 饶微袁锦云刘坤刘来壮何伟峰詹秀玉
Owner SHENZHEN NEW INDS BIOMEDICAL ENG
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