Synergistic application of farnesol on methoxy acrylic fungicides to prevention and treatment of smut disease

A technology of methoxyacrylic acid and farnesol, which is applied in the direction of fungicides, applications, biocides, etc., can solve the problems of weakened bactericidal effect and increased field usage, so as to reduce the usage amount, improve the bactericidal ability, and improve the antibacterial effect. The effect of bacteria

Active Publication Date: 2018-08-28
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the widespread use of this chemical agent, its resistance continues to develop, and its bactericidal effect is weakening day by day

Method used

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  • Synergistic application of farnesol on methoxy acrylic fungicides to prevention and treatment of smut disease
  • Synergistic application of farnesol on methoxy acrylic fungicides to prevention and treatment of smut disease
  • Synergistic application of farnesol on methoxy acrylic fungicides to prevention and treatment of smut disease

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0032] On the basis of the preliminary test, farnesol and 25% azoxystrobin suspension were prepared in proportion to a certain concentration gradient mother solution for use (respectively 0.08μM, 0.1μM, 0.2μM, 0.3μM, 0.4μM, 0.5μM farnesol , azoxystrobin diluted 4×10 4times, 0.04μM farnesol + azoxystrobin diluted 8 × 10 4 times, 0.05μM farnesol+azoxystrobin diluted 8×10 4 times, 0.1 μM farnesol + azoxystrobin diluted 8 × 10 4 times, 0.15μM farnesol + azoxystrobin diluted 8 × 10 4 times, 0.2 μM farnesol + azoxystrobin diluted 8 × 10 4 times, 0.25μM farnesol + azoxystrobin diluted 8 × 10 4 times). Pick a few basidiospores from the plate and culture overnight in 6mL YEPS culture medium as the seed solution, collect the bacteria by centrifugation the next day, and resuspend the bacteria in YEPS liquid medium (OD600 is 0.2) for later use. Pipette 300 μL of different concentrations of mother liquor into 2 mL sterilized centrifuge tubes, add the same amount of basidiospore suspe...

example 2

[0035] On the basis of the preliminary test, farnesol and 25% azoxystrobin suspension were prepared in proportion to a certain concentration gradient mother solution for use (farnesol: 0.1 μM, 0.2 μM, 0.3 μM, 0.5 μM, azoxystrobin: Dilute 4×10 4 times). Pick a few basidiospores from the plate and culture overnight in 6mL YEPS culture medium as seed liquid, transfer 500μL to 50mL YEPS culture medium for expansion culture (to OD600 is 0.2) the next day, absorb 500μL of different concentrations of mother solution to 5mL sterilized centrifuge In the tube, add an equal volume of bacterial solution and mix well, and record it as the control group used alone; in addition, draw 250 μL of mother solutions of different concentrations of farnesol and 250 μL of azoxystrobin mother solutions and mix them in a 5 mL sterilized centrifuge tube, add an equal volume of bacterial solution Mix well, and record it as the mixed group; pipette 500 μL of YEPS into a 5 mL sterilized centrifuge tube, a...

example 3

[0039] On the basis of the preliminary test, farnesol and 25% azoxystrobin suspension concentrate were prepared in proportion to a certain concentration gradient mother solution for use (farnesol: 0.08μM, azoxystrobin: diluted 4×10 4 times). Pick a few basidiospores from the plate and culture overnight in 6mL YEPS culture medium as the seed liquid, transfer 500 μL to 50mL YEPS culture medium the next day for expansion culture (to OD600 of 0.2) for use. The concentration of the azoxystrobin mother solution was diluted sequentially to 0.5 times, 0.4 times, 0.3 times, 0.2 times and 0.1 times. Take 250 μL of farnesol mother solution and the same volume of azoxystrobin with different concentration gradients and mix them in a centrifuge tube, and use 500 μL of farnesol and azoxystrobin mother solution as a separate use group; 500 μL of YEPS as a blank control, set up repetitions, Add 500 μL of bacterial solution to all centrifuge tubes and mix well, place them in a shaker at 28°C a...

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Abstract

The invention discloses new applicationfor fungicidal synergism of farnesol and methoxy acrylic fungicides to prevention and treatment of smut disease. The synergistic combination can be applied to prevention and treatment of sugarcane, corn and other crops caused by sporosorium and ustilago, has a high synergistic effect, and can significantly reduce the use of chemical pesticides, and the prevention and treatment effect is significantly better than that of a single agent.

Description

technical field [0001] The invention relates to the technical field of biological control, in particular to the synergistic effect of farnesol and methoxyacrylic fungicides, and is mainly used in the prevention and treatment of sugarcane smut and corn smut Other crop smut caused by the genus. Background technique [0002] Smut is a plant disease mainly caused by smut. According to incomplete statistics, there are about 1,200 species of smut, which can infect more than 4,000 kinds of plants. Their hosts include grass plants and important crops in the world such as corn. , sugarcane, barley, wheat, oats, sorghum, etc. Smut is characterized by the formation of mounds of black powdery teliospores on the tissue of the infested host. [0003] Sugarcane is the main raw material for the sugar industry, and corn is the main food crop. They are also important feed crops and biomass energy materials. Ustilago (sporisorium) Sugarcane smut and Ustilago spp (Ustilago) Causes maize s...

Claims

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Application Information

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IPC IPC(8): A01N31/02A01N43/54A01N25/04A01P3/00
CPCA01N25/04A01N31/02A01N43/54
Inventor 常长青仇善旭蔡恩平吴熔熔王雨婷王金佩张炼辉
Owner SOUTH CHINA AGRI UNIV
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