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Application of Kartogenin for preparing drugs for improving mesenchymal stem cell oxidation resistance

A technology of antioxidant capacity and bone marrow mesenchyme, which is applied in the field of medicine, can solve problems such as the impact of BM-MSC antioxidant capacity, and achieve the effects of enhancing cell antioxidant capacity, slowing down development, and improving antioxidant capacity

Inactive Publication Date: 2018-08-28
THE FIRST AFFILIATED HOSPITAL OF SOOCHOW UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is still no relevant data reporting the effect of KGN on the antioxidant capacity of BM-MSC in the treatment of osteoarthritis.

Method used

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  • Application of Kartogenin for preparing drugs for improving mesenchymal stem cell oxidation resistance
  • Application of Kartogenin for preparing drugs for improving mesenchymal stem cell oxidation resistance
  • Application of Kartogenin for preparing drugs for improving mesenchymal stem cell oxidation resistance

Examples

Experimental program
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Effect test

Embodiment 1

[0036] Resuscitate bone marrow mesenchymal stem cells, inoculate at 175cm 2 Add 20mL complete medium to the culture flask and place it at 37℃, 5% CO 2 Incubate in an incubator and change the medium every three days. The cells in the logarithmic growth phase were seeded on multiple 6-well culture plates, and complete medium was added, and the medium was changed every three days. After the cell density is greater than 90%, add complete medium (control group), 0.005% DMSO, 10nM KGN, 100nM KGN, 1000nM KGN after grouping. After incubating for one day, determine the concentration of reactive oxygen species and antioxidant enzymes (SOD). And CAT) activity.

Embodiment 2

[0038] Resuscitate bone marrow mesenchymal stem cells, inoculate at 175cm 2 Add 20mL complete medium to the culture flask and place it at 37℃, 5% CO 2 Incubate in an incubator and change the medium every three days. The cells in the logarithmic growth phase were seeded on multiple 6-well culture plates, and complete medium was added, and the medium was changed every three days. After the cell density is greater than 90%, add complete medium (control group), 0.005% DMSO, 10nM KGN, 100nM KGN, and 1000nM KGN after grouping. After incubating for three days, the expression of antioxidant-related marker genes in the cells is determined.

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Abstract

The invention belongs to the field of medicine, and discloses application of Kartogenin for preparing drugs for improving the mesenchymal stem cell oxidation resistance. An experiment shows that KGN can reduce active oxygen inside BM-MSC, can improve the SOD and CAT activity, and can improve the gene expression level of SOD1, SOD2, CAT and GPX1 and increase the expression content of correspondingprotein. Accordingly, it proves that KGN has the promoting effect on the intracellular anti-oxidant function of stem cells, and the application of Kartogenin for preparing the drugs for improving themesenchymal stem cell oxidation resistance can provide a brand new scheme for improving the body cell oxidation resistance for medicine, tissue engineering research and the like.

Description

Technical field [0001] The present invention belongs to the field of medicine, and particularly relates to the application of Kartogenin (hereinafter referred to as KGN) in the preparation of drugs for improving the antioxidant capacity of bone marrow mesenchymal stem cells. Background technique [0002] Bone marrow mesenchymal stem cells (BM-MSC) exist in human bone marrow and are a type of adult stem cells. In terms of bone biology, BM-MSC can differentiate into chondrocytes and osteoblasts under specific induction conditions. Because of its multi-directional differentiation potential, wide sources, and high proliferation characteristics, BM-MSC has become an important source of target cells in the field of tissue engineering and genetic engineering. [0003] Reactive oxygen species (ROS) is an oxygen reduction product with high reactivity. The normal concentration of ROS in the body can maintain the steady state of the cell environment and ensure the orderly progress of cell di...

Claims

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Application Information

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IPC IPC(8): A61K31/196A61P39/06
CPCA61K31/196
Inventor 王一帆何帆陈曦闫金库李茂陈广东张文赵环刘滔杨惠林
Owner THE FIRST AFFILIATED HOSPITAL OF SOOCHOW UNIV
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