Piezoelectric ultrasonic cutting system and method for oocyte zona pellucida

Abstract

The invention discloses a piezoelectric ultrasonic cutting system and method for oocyte zona pellucida. The piezoelectric ultrasonic cutting system comprises an oocyte posture adjusting chip, an oocyte holding device and an oocyte ultrasonic cutting scalpel, wherein the oocyte posture adjusting chip is used for performing posture adjustment and bottom fixation and holding on oocytes; the oocyte holding device comprises a bent holding micro-needle and a micro-needle clamp used for clamping the bent holding micro-needle; the end of the bent holding micro-needle is provided with a single-side inwards recessed arc holding needle head; a bayonet is formed inside the bent holding micro-needle, and a notch is formed in the end of the bent holding micro-needle; the oocyte ultrasonic cutting scalpel is used for cutting the oocyte zona pellucida from the notch position. According to the piezoelectric ultrasonic cutting system disclosed by the invention, the defect that the oocytes are damaged due to longitudinal conduction of ultrasonic energy can be effectively overcome, the zona pellucida is cut from a tangential direction of the oocyte surface layer by the oocyte ultrasonic cutting scalpel, and damage of the oocytes can be reduced.

Description

technical field [0001] The invention relates to the technical field of biological micromanipulation, in particular to a piezoelectric ultrasonic cutting system and method for oocyte zona pellucida. Background technique [0002] Oocyte is the most important oocyte in the process of life gestation, which contains important genetic material of a living body. Therefore, the micromanipulation technology of oocyte has always occupied the most important position in life science research and related medical technology. important position. With the increasing rate of infertility around the world and the continuous pursuit of eugenics and prenatal care, artificial assisted reproductive technology has developed to the third generation of test-tube baby technology. Holes are made in the zona pellucida of the embryo, and then a single embryonic leaf oocyte is taken from the eight-oocyte stage on the third day or several trophoblast oocytes are taken from the blastocyst on the fifth day ...

AI Technical Summary

Problems solved by technology

[0004] The first is to use a laser to drill holes in the oocytes, and then use micro-needles to absorb the contents of the oocytes. Although this method has good experimental results, the oocytes have a high survival rate and no pollution after manipulation, but The disadvantage is that the equipment is expensive and cannot be used in routine operations in hospitals and research institutes;

[0005] The second method is to use piezoelectric ultrasonic micromanipulation needles to "puncture holes". In this method, under the perspective of the microscope, the suction needle is firstly fixed at the nine o'clock direction of the oocyte, and then the operation needle is used to fix the oocyte in the direction of the piezoelectric micromanipulator. Under the ultrasonic vibration of ceramics, ultrasonic energy is used to drill holes in oocytes, and then use micro-pointers to extract the contents of oocytes. This method requires researchers to have rich operating experience, and due to the ultrasonic longitudinal conductivity Therefore, during the experiment, it is easy to cause damage to the internal structure of the oocyte due to excessive ultrasonic energy

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