Duck adenovirus 2 and DPV (Muscovy duck parvovirus) disease combined inactivated vaccine

A Muscovy duck parvovirus and dual vaccine technology, which is applied in the direction of viruses, vaccines, antiviral agents, etc., can solve the problems of poor immune effect, stress response of Muscovy duck flocks, virus mutation, etc., and achieve good commercial development Promising, effective immune protection, good safety profile

Active Publication Date: 2018-08-31
SHANDONG SINDER TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the commercialized inactivated vaccines for the prevention of these two diseases at home and abroad are not very effective in immunization. Individual duck farms still have symptoms of disease and even death, which may be related to the mutation of the virus. It is necessary to screen the mutated virus strains to prepare vaccine
In addition, multiple injections and multiple doses of a single vaccine will bring some unnecessary stress reactions to the Muscovy ducks

Method used

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  • Duck adenovirus 2 and DPV (Muscovy duck parvovirus) disease combined inactivated vaccine
  • Duck adenovirus 2 and DPV (Muscovy duck parvovirus) disease combined inactivated vaccine
  • Duck adenovirus 2 and DPV (Muscovy duck parvovirus) disease combined inactivated vaccine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Example 1 Isolation and Identification of Virus Strains

[0017] 1.1 Isolation and identification of GD strain

[0018] 1.1.1 Virus isolation of GD strain In 2015, the inventor successfully isolated a duck adenovirus type 2 from a group of muscovy ducks in a duck farm in Guangdong. Take the liver, spleen, kidney and other tissues of the diseased Muscovy duck, grind them, homogenize them with sterile PBS, freeze and thaw 3 times, and then centrifuge at 6000r / min for 10 minutes. Take the supernatant and add penicillin and streptomycin at 4°C overnight. After passing the filter sterility test, save it for future use. Muscovy duck embryo fibroblasts were inoculated with 1% virus filtrate, cultured in serum-free M199 culture medium, left standing at 37°C for 60-78 hours, obvious cytopathic changes could occur, and the virus fluid was collected.

[0019] 1.1.2 Identification of etiology The collected virus liquid was purified and analyzed for virus characteristics in terms ...

Embodiment 2

[0031] Embodiment 2 Vaccine manufacture and semi-finished product inspection

[0032] 2.1 Preparation of virus seeds

[0033] 2.1.1 Preparation of virus seed of GD strain Select muscovy duck embryo fibroblasts that have overgrown a single layer, discard the original culture medium, and add serum-free M199 with a final concentration of 1% virus seed (the third generation of the original virus seed of GD strain) Maintenance solution, static culture at 37°C for 60-78 hours, obvious cytopathy may appear, harvest when the cytopathy reaches more than 80%. According to this method, 10 generations were passed continuously, which were respectively marked as C3-C10 generations. The virus content of each passage was determined. The same generation will be tested for sterility and virus content ≥ 10 6.0 TCID 50 / 0.1ml virus solution, mixed quantitatively, and freeze-dried for storage.

[0034] 2.1.2 Preparation of virus seed of MDPV-GDL strain The allantoic fluid (the third generatio...

Embodiment 3

[0060] Example 3 Vaccine finished product inspection

[0061] 3.1 Traits

[0062] Appearance Milky white emulsion.

[0063] Dosage form water-in-oil type. Take a clean straw, suck a small amount of vaccine and drop it into cold water, except for the first drop, it should not spread.

[0064] Stability Take 10ml of the vaccine and put it into a centrifuge tube, centrifuge at 3000r / min for 15 minutes, the water precipitated at the bottom of the tube should not exceed 0.5ml.

[0065] Viscosity is carried out according to the appendix of the current "Chinese Veterinary Pharmacopoeia", and it should meet the requirements.

[0066] 3.2 Inspection of filling volume Inspection shall be carried out according to the appendix of the current "Chinese Veterinary Pharmacopoeia", and it shall meet the regulations.

[0067] 3.3 Sterility test According to the current "Chinese Veterinary Pharmacopoeia" appendix to test, should be sterile growth.

[0068] 3.4 Safety inspection In order to ...

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Abstract

The invention provides a duck adenovirus 2 and DPV (Muscovy duck parvovirus) disease combined inactivated vaccine. Antigens are inactivated duck adenovirus 2 and DPV, wherein the preservation number of the DPV is CCTCC NO:V201812, and the preservation number of the duck adenovirus 2 is CCTCC No:V201633. The prepared duck adenovirus 2 and DPV disease combined inactivated vaccine is good in safety and any local and general side effects caused by the vaccine are prevented. The analysis for character, safety test and efficacy test data in retention period tests indicates that all indexes are stable and effective; the immune effect of the vaccine is evaluated with a serological method and an immune challenge method, a result shows that the combined inactivated vaccine can provide effective immune protection for ducks and has good commercialized development prospect.

Description

technical field [0001] The invention belongs to the technical field of veterinary biological products, and in particular relates to the preparation and application of a dual inactivated vaccine of duck type 2 adenovirus and muscovy duck parvovirus disease. Background technique [0002] Adenoviruses are common infectious pathogens of poultry. Avian adenoviruses are divided into three groups: Group I is avian adenoviruses isolated from respiratory infections in chickens, turkeys, geese and ducks. They have common group-specific antigens and can be divided into A, B, C, D, E5 species and 12 serotypes; Group II includes turkey hemorrhagic enteritis virus, pheasant marble skin disease virus and chicken large spleen disease virus; Group III is an adenovirus isolated from chicken laying syndrome and ducks. Duck adenovirus type 2 belongs to Group I poultry adenovirus. It was isolated from sick Muscovy ducks by French-Hungarians in 1977. The infected ducks mainly manifested symptoms...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/295A61K39/235A61K39/23A61P31/20C12N7/00
CPCA61K39/12A61K2039/5252A61K2039/552A61K2039/70C12N7/00C12N2710/10234C12N2710/10251C12N2750/14034C12N2750/14051
Inventor 王相芹王丹娜刘阳孙娜娜林文超王学波李朝阳
Owner SHANDONG SINDER TECH
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