Confocal microscope system based on optical fiber coupler

Abstract

The invention provides a confocal microscope system based on an optical fiber coupler, comprising a light source, an optical fiber coupling unit, a scanning unit, a microscopy imaging unit and a detection unit, wherein the light source is used for generating coherent light and transmitting the coherent light to the optical fiber coupling unit; the optical fiber coupling unit is used for transmitting the coherent light to the scanning unit and transmitting reflected light or fluorescent light returned by the scanning unit to the detection unit; the scanning unit is used for receiving the coherent light and transmitting to the microscopy imaging unit, and receiving the reflected light or fluorescent light returned by the microscopy imaging unit and transmitting to the optical fiber couplingunit; the microscopy imaging unit is used for gathering the coherent light to the surface of a sample, and transmitting the reflected light or fluorescent light of the sample to the scanning unit; andthe detection unit is used for collecting the reflected light or the fluorescent light. The optical fiber coupler is used for separating mainframe parts such as laser and a detector from scanning andmicroscopy imaging parts, so as to shorten the patient waiting time.

Description

technical field [0001] The invention relates to the technical field of microscopic imaging, and more specifically, to a confocal microscopic system based on a fiber coupler. Background technique [0002] Laser confocal microscope is a highly integrated optical microscope, which plays an extremely important role in the morphology research of biological science. The basic principle is to use laser as the light source, and use conjugate focusing technology to eliminate the interference of stray light outside the focal point, which greatly improves the resolution. 3D imaging and optical sectioning are possible with fast scanning imaging and Z-axis stepping. [0003] On the basis of microscopic imaging, the confocal microscope adds a laser scanning device, which is an advanced cell and molecular biology analysis instrument that combines laser, electronics, and computer imaging technology. On the basis of traditional optical microscopes, laser confocal microscopes use laser ligh...

AI Technical Summary

Problems solved by technology

[0005] The present invention provides a fiber coupler-based confocal microscope system that overcomes the above-mentioned problems or at least partially solves the above-mentioned problems, and solves the problem that the confocal microscope system in the prior art is complicated in structure, too large in size, and difficult to in vivo. The problem of real-time observation of patient tissue

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