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Preparation method of BSA-EGCG-TPS nano particles

A nanoparticle and suspension technology, applied in the field of nanoparticle technology application, can solve the problems of limiting the full utilization of EGCG, low bioavailability, low absorption rate, etc., and achieve high encapsulation efficiency and drug loading, high biological activity, The effect of improving stability

Active Publication Date: 2018-09-07
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the absorption rate of EGCG in the human body is less than 5%, and it is less than 1% after oral administration; moreover, EGCG is very easily oxidized
The shortcomings of poor stability, low bioavailability and high toxicity limit the full utilization of EGCG

Method used

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  • Preparation method of BSA-EGCG-TPS nano particles

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] A method for preparing BSA-EGCG-TPS nanoparticles includes the following steps:

[0028] (1) Preparation of tea polysaccharides: take green tea powder, extract with an 80% ethanol aqueous solution with a volume concentration of 5 times the mass of green tea powder for 24 hours, filter, dry the residue, and extract with water at 95°C equivalent to 20 times the mass of the residue The residue was filtered and extracted 3 times for 2h. The water-extracted filtrates were combined and concentrated to 1 / 8 of the volume of the combined water-extracted filtrates to obtain a concentrated solution. The volume concentration of the concentrated solution was 4 times the volume of the concentrated solution. Placed for 10 hours at ℃, centrifuged and freeze-dried the solid to obtain crude tea polysaccharide; then purified by polyamide column chromatography and ultrafiltration to obtain tea polysaccharide with a molecular weight of 30-80 KDa, which is referred to as TPS.

[0029] (2) Drop 50...

Embodiment 2

[0034] A method for preparing BSA-EGCG-TPS nanoparticles includes the following steps:

[0035] (1) Preparation of tea polysaccharides: Take green tea powder, extract with 85% ethanol aqueous solution with a volume concentration of 4 times the mass of green tea powder for 20 hours, filter, dry the residue, and extract 15 times the mass of the residue with water at 100°C The residue was filtered and extracted twice for 1.5h, combined the water-extracted filtrate, concentrated to 1 / 10 of the volume of the combined water-extracted filtrate to obtain a concentrated solution, and added a 95% ethanol aqueous solution with a volume concentration equivalent to 4 times the volume of the concentrated solution to precipitate. Placed at 4°C for 8 hours, centrifuged and freeze-dried the solid to obtain crude tea polysaccharide; then purified by polyamide column chromatography and ultrafiltration to obtain tea polysaccharide with a molecular weight of 30-80KDa, which is referred to as TPS.

[00...

Embodiment 3

[0041] A method for preparing BSA-EGCG-TPS nanoparticles includes the following steps:

[0042] (1) Preparation of tea polysaccharides: take green tea powder, extract with a 75% ethanol aqueous solution with a volume concentration of 6 times the mass of green tea powder for 28 hours, filter, dry the residue, and extract with water at 90°C equivalent to 25 times the mass of the residue The residue was filtered and leached 4 times for 2.5 hours. The water-extracted filtrates were combined and concentrated to 1 / 5 of the volume of the combined water-extracted filtrates to obtain a concentrated solution. The volumetric concentration of the concentrated solution was 4 times the volume of the concentrated solution. Placed at 4°C for 12 hours, centrifuged, and freeze-dried the solid to obtain crude tea polysaccharides; then purified by polyamide column chromatography and ultrafiltration to obtain tea polysaccharides with a molecular weight of 30-80 KDa, which is abbreviated as TPS.

[0043...

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Abstract

The invention discloses a preparation method of BSA-EGCG-TPS nano particles, comprising the following steps: dissolving BSA into water to prepare a BSA aqueous solution, dropwise dropping absolute ethyl alcohol to the BSA solution, stirring, standing, dropwise dropping glutaraldehyde, stirring, centrifuging, washing with water and precipitating, re-suspending precipitates to water to obtain BSA nano particle suspension liquid; dissolving EGCG in water to prepare an EGCG aqueous solution, dropwise dripping into the BSA nano particle suspension liquid, stirring to obtain BSA-EGCG nano particle suspension liquid; dissolving tea polysaccharide TPS in water to prepare a tea polysaccharide aqueous solution, dropwise dripping into the BSA-EGCG nano particle suspension liquid, stirring, centrifuging, washing with water, and performing freeze drying, thus obtaining BSA-EGCG-TPS nano particles BETN. According to the preparation method, the preparation process is simple, convenient, free from toxicity, and safe, and the obtained nano particles are high in stability and good in antioxidant activity.

Description

Technical field [0001] The invention relates to a method for preparing BSA-EGCG-TPS nano particles, and belongs to the technical application field of nano particles. Background technique [0002] EGCG is epigallocatechin gallate. It is the main component of green tea polyphenols and is a flavanol-3-ol. EGCG has many functions such as anti-oxidation, anti-tumor and anti-diabetic. However, the absorption rate of EGCG in the human body is less than 5% and less than 1% after oral administration; moreover, EGCG is very easily oxidized. The shortcomings of poor stability, low bioavailability and high toxicity limit the full utilization of EGCG. Tea polysaccharide is another important active ingredient in tea, and it is also a potential functional food. [0003] BSA, or bovine serum albumin, has the characteristics of non-toxicity, non-immunogenicity, low cost, good biocompatibility and biodegradability. It can be used as a multifunctional protein carrier in drug delivery systems. In ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/51A61K47/42A61K47/36A61K31/353A61P39/06A61P35/00A23L33/105A23L33/125A23L33/17
CPCA23L33/105A23L33/125A23L33/17A23V2002/00A61K9/5161A61K9/5169A61K31/353A61P35/00A61P39/06A23V2250/21A23V2250/214A23V2250/51A23V2250/5436
Inventor 陈海霞李伟伟潘宇翔袁国淇
Owner TIANJIN UNIV