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A kind of preparation method of bsa-egcg-tps nano particle

A nanoparticle and suspension technology, applied in the application field of nanoparticle technology, can solve the problems of limiting the full utilization of EGCG, low bioavailability, poor stability, etc., and achieves high encapsulation efficiency and drug loading, high biological activity, and stability high sex effect

Active Publication Date: 2020-04-28
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the absorption rate of EGCG in the human body is less than 5%, and it is less than 1% after oral administration; moreover, EGCG is very easily oxidized
The shortcomings of poor stability, low bioavailability and high toxicity limit the full utilization of EGCG

Method used

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  • A kind of preparation method of bsa-egcg-tps nano particle

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Experimental program
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Effect test

Embodiment 1

[0027] A preparation method for BSA-EGCG-TPS nanoparticles, comprising the steps of:

[0028] (1) Preparation of tea polysaccharide: take green tea powder, extract with 80% ethanol aqueous solution with a volume concentration equivalent to 5 times the mass of green tea powder for 24 hours, filter, dry the residue, and extract with 95°C water equivalent to 20 times the mass of the residue The residue was filtered for 2 hours, leached 3 times, and the combined water-extracted filtrate was concentrated to 1 / 8 of the volume of the combined water-extracted filtrate to obtain a concentrated solution. Add a 95% aqueous ethanol solution with a volume concentration equivalent to 4 times the volume of the concentrated solution. Precipitate, 4 ℃, placed for 10 hours, centrifuged, and solid freeze-dried to obtain crude tea polysaccharide; then purified by polyamide column chromatography and ultrafiltration to obtain tea polysaccharide with a molecular weight of 30-80KDa, which is referred ...

Embodiment 2

[0034] A preparation method for BSA-EGCG-TPS nanoparticles, comprising the steps of:

[0035] (1) Preparation of tea polysaccharide: take green tea powder, leaching for 20 hours with 85% ethanol aqueous solution equivalent to 4 times the mass of green tea powder, filter, dry the residue, and extract with 100°C water equivalent to 15 times the mass of the residue The residue was filtered for 1.5 hours, leached twice, the combined water-extracted filtrate was concentrated to 1 / 10 of the volume of the combined water-extracted filtrate to obtain a concentrated solution, and an aqueous solution of 95% ethanol with a volume concentration equivalent to 4 times the volume of the concentrated solution was added to precipitate. Place at 4°C for 8 hours, centrifuge, freeze-dry the solid to obtain crude tea polysaccharide; then purify by polyamide column chromatography and ultrafiltration to obtain tea polysaccharide with a molecular weight of 30-80KDa, which is referred to as TPS.

[003...

Embodiment 3

[0041] A preparation method for BSA-EGCG-TPS nanoparticles, comprising the steps of:

[0042] (1) Preparation of tea polysaccharide: take green tea powder, leaching for 28 hours with ethanol aqueous solution with a volume concentration of 75% equivalent to 6 times the mass of green tea powder, filter, dry the residue, and extract with 90°C water equivalent to 25 times the mass of the residue The residue was filtered for 2.5 hours, leached 4 times, the combined water-extracted filtrate was concentrated to 1 / 5 of the volume of the combined water-extracted filtrate to obtain a concentrated solution, and a 95% aqueous ethanol solution with a volume concentration equivalent to 4 times the volume of the concentrated solution was added to precipitate. Place at 4°C for 12 hours, centrifuge, freeze-dry the solid to obtain crude tea polysaccharide; then purify by polyamide column chromatography and ultrafiltration to obtain tea polysaccharide with a molecular weight of 30-80KDa, which is...

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Abstract

The invention discloses a preparation method of BSA-EGCG-TPS nano particles, comprising the following steps: dissolving BSA into water to prepare a BSA aqueous solution, dropwise dropping absolute ethyl alcohol to the BSA solution, stirring, standing, dropwise dropping glutaraldehyde, stirring, centrifuging, washing with water and precipitating, re-suspending precipitates to water to obtain BSA nano particle suspension liquid; dissolving EGCG in water to prepare an EGCG aqueous solution, dropwise dripping into the BSA nano particle suspension liquid, stirring to obtain BSA-EGCG nano particle suspension liquid; dissolving tea polysaccharide TPS in water to prepare a tea polysaccharide aqueous solution, dropwise dripping into the BSA-EGCG nano particle suspension liquid, stirring, centrifuging, washing with water, and performing freeze drying, thus obtaining BSA-EGCG-TPS nano particles BETN. According to the preparation method, the preparation process is simple, convenient, free from toxicity, and safe, and the obtained nano particles are high in stability and good in antioxidant activity.

Description

technical field [0001] The invention relates to a preparation method of BSA-EGCG-TPS nano particles, which belongs to the field of nano particle technology application. Background technique [0002] EGCG is epigallocatechin gallate, which is the main component of green tea polyphenols and is a kind of flavanol-3-ol. EGCG has various effects such as anti-oxidation, anti-tumor, and anti-diabetes. However, the absorption rate of EGCG in the human body is less than 5%, and it is less than 1% after oral administration; moreover, EGCG is very easily oxidized. The shortcomings of poor stability, low bioavailability and high toxicity limit the full utilization of EGCG. Tea polysaccharide is another important active ingredient in tea and a potential functional food. [0003] BSA, bovine serum albumin, has the characteristics of non-toxicity, non-immunogenicity, low cost, good biocompatibility and biodegradability, and it can be used as a multifunctional protein carrier in drug del...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/51A61K47/42A61K47/36A61K31/353A61P39/06A61P35/00A23L33/105A23L33/125A23L33/17
CPCA23L33/105A23L33/125A23L33/17A23V2002/00A61K9/5161A61K9/5169A61K31/353A61P35/00A61P39/06A23V2250/21A23V2250/214A23V2250/51A23V2250/5436
Inventor 陈海霞李伟伟潘宇翔袁国淇
Owner TIANJIN UNIV