Bi-crosslinking mussel binding protein bionic hydrogel for tissue adhesive and preparation method of bi-crosslinking mussel binding protein bionic hydrogel

A tissue adhesive and double-crosslinking technology, which is applied in the field of double-crosslinked mussel binding protein biomimetic hydrogel and its preparation, can solve problems such as low bonding strength or mechanical strength, and reduce efficacy, and achieve crosslinking Fast speed, good adhesion and good biocompatibility

Inactive Publication Date: 2018-09-07
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these hydrogels tend to swell or dissolve quickly, or have low cohesive or m

Method used

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  • Bi-crosslinking mussel binding protein bionic hydrogel for tissue adhesive and preparation method of bi-crosslinking mussel binding protein bionic hydrogel
  • Bi-crosslinking mussel binding protein bionic hydrogel for tissue adhesive and preparation method of bi-crosslinking mussel binding protein bionic hydrogel
  • Bi-crosslinking mussel binding protein bionic hydrogel for tissue adhesive and preparation method of bi-crosslinking mussel binding protein bionic hydrogel

Examples

Experimental program
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Example Embodiment

[0030] Example 1

[0031] (1) Weigh 0.5 gram of chitosan, add 50 milliliters of deionized water that adopts 1mol / L hydrochloric acid to adjust the pH value to 5, stir at room temperature, until forming a colorless or light yellow transparent solution, then add 1.72mmol of 3 , 4-dihydroxyphenylpropionic acid, stir and mix evenly, add 1-ethyl-(3-dimethylaminopropyl) dissolved in 50ml deionized water and ethanol mixed solution (v:v=1:1) ) carbodiimide hydrochloride (10.32mmol) solution, adjust the pH value of the reaction solution to 4-5, react at room temperature for 12 hours, dialyze in ultrapure water for 3 days, freeze-dry to obtain dopa residue modified chitosan sugar.

[0032] (2) Get 0.5 grams of dopa residue modified chitosan in step (1) and dissolve in 50 milliliters of deionized water that adopts 1mol / L hydrochloric acid to adjust the pH value to 5, stir and dissolve at room temperature, then add 5.16mmol N-acetylcysteine ​​and 10.32mmol of 1-ethyl-(3-dimethylaminopro...

Example Embodiment

[0035] Example 2

[0036] (1) Take 1.0 gram of chitosan in 100 milliliters of deionized water that adopts 1mol / L hydrochloric acid to adjust the pH value to 5, stir at room temperature until a colorless or light yellow transparent solution is formed, then add 3.44mmol of 3, 4-dihydroxyphenylpropionic acid, stir and mix evenly, add 1-ethyl-(3-dimethylaminopropyl)carbon dissolved in 100ml deionized water and ethanol mixed solution (v:v=1:1) Diimine hydrochloride (20.64mmol) solution, adjust the pH value of the reaction solution to 4-5, react at room temperature for 24 hours, dialyze in ultrapure water for 3 days, freeze-dry to obtain dopa residue modified chitosan.

[0037] (2) get 0.5 gram of dopa residue modified chitosan prepared in step (1) and dissolve in 50 milliliters of deionized water that adopts 1mol / L hydrochloric acid to adjust the pH value to 5, stirring and dissolving at room temperature, then adding 5.16mmol of N-acetylcysteine ​​and 10.32mmol of 1-ethyl-(3-dimet...

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Abstract

The invention relates to a bi-crosslinking mussel binding protein bionic hydrogel for a tissue adhesive and a preparation method of the bi-crosslinking mussel binding protein bionic hydrogel. The hydrogel is obtained by taking mercapto group modified chitosan containing dopa residues as a matrix and performing bi-crosslinking under the condition of four-arm polyethyleneglycol succinimidyl carbonate and sodium periodate. The preparation method comprises the following steps of preparation of dopa residue modified chitosan, preparation of dopa residue and mercapto group modified chitosan, preparation of four-arm polyethyleneglycol succinimidyl carbonate and preparation of the bi-crosslinking mussel binding protein bionic hydrogel. In the preparation method provided by the invention, by takingthe sodium periodate as a crosslinking agent which performs rapid crosslinking and the four-arm polyethyleneglycol succinimidyl carbonate as a crosslinking agent which is relatively slow in reaction,the hydrogel is prepared by bi-crosslinking, the crosslinking speed is rapid, and the prepared hydrogel has very good adhesion performance, biocompatibility and mechanical strength and is hydrogel which can be used for the tissue adhesive.

Description

technical field [0001] The invention belongs to the field of hydrogel and its preparation, in particular to a double-crosslinked mussel binding protein biomimetic hydrogel used for tissue adhesives and a preparation method thereof. Background technique [0002] Traditional mechanical wound closure devices, such as paper clips, rivets, and surgical sutures, often cause damage to the tissues around the wound, and are not suitable for organs such as the liver, spleen, and kidney with low cohesive strength, and these wound closure devices The operation in the body is complicated and requires certain skills, and a second operation is required to remove it. However, tissue adhesives can effectively solve or avoid these problems. When tissue adhesives restore the function of damaged parts, they can also restore their appearance before the damage. They have good biodegradability and do not require secondary This operation greatly reduces the pain of patients and the workload of doc...

Claims

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Application Information

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IPC IPC(8): C08J3/075C08J3/24C08L5/08C08L71/02C08B37/08C08G65/333A61L24/00A61L24/08A61L24/04
CPCA61L24/0031A61L24/046A61L24/08C08B37/003C08G65/33337C08J3/075C08J3/246C08J2305/08C08J2371/02C08J2405/08C08J2471/02
Inventor 莫秀梅曾志文
Owner DONGHUA UNIV
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