SNP locus relevant to rapid growth of crassostrea gigas

A long oyster, site technology, applied in the field of genetic breeding, can solve the problems of slow growth, long oyster germplasm degradation, red tide, etc., achieve the effect of fast growth and reduce blindness

Active Publication Date: 2018-09-14
ZHEJIANG WANLI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, while my country's long oyster aquaculture industry is developing steadily, it is also beginning to face many serious problems: with the continuous improvement of aquaculture intensification and the frequent occurrence of marine environmental pollution and red tides, the aquaculture environment of long oysters is deteriorating day by day; Breeding leads to inbreeding, and the germplasm degradation of cultured oysters is becoming more and more serious. Problems such as slow growth, low meat yield, and increased mortality have occurred, which seriously affect the sustainable development of the oyster industry; with the improvement of consumption levels and the market With the intensification of competition, the domestic and foreign consumer markets have continuously improved the quality requirements of long oysters

Method used

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  • SNP locus relevant to rapid growth of crassostrea gigas
  • SNP locus relevant to rapid growth of crassostrea gigas
  • SNP locus relevant to rapid growth of crassostrea gigas

Examples

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Effect test

Embodiment 1

[0017] Example 1: Acquisition of markers associated with rapid growth of the long oyster

[0018] 1. On the basis of the complete sequencing of the long oyster genome, the applicant obtained the growth-related genes—bHLH family through GWAS association analysis technology. The bHLH family of oyster oyster was classified, including 89 genes from groups A to F. On this basis, 5 candidate genes were selected for SNP screening by resequencing, and a wild population of 417 individuals was selected. The obtained 63 loci were verified by gene chip method in the wild population of 288 individuals, and finally the correlation analysis with growth traits was carried out with biological software to obtain the NewChr4_588155SNP marker. The GG shell width of NewChr4_588155-marked genotype individuals is significantly larger than that of TT genotype and TG genotype individuals, and it is located in the exon of gene LOB (SEQ ID NO: 1), which changes the composition of the amino acid sequence...

Embodiment 2

[0026] Example 2: Application of SNP markers in screening fast-growing long oyster parents

[0027] A kit for evaluating and screening the SNP markers of fast-growing long oyster parents was made to detect the SNP site NewChr4_588155 of the LOB gene.

[0028] The kit contains reagents for specific amplification primers and specific extension primers required to detect SNP sites, wherein the specific amplification primer sequences for site NewChr4_588155 are SEQ ID NO: 2 and SEQ ID NO: 3, the The kit also includes common reagents required by corresponding techniques such as (10×PCR (containing Mg2+) buffer, dNTP (10mM), double distilled water, enzymes, etc. In addition, there may be standard products and controls such as determined genes).

[0029] The amplification reaction system includes: 1.0 μL of cDNA, 2.5 μL of 10×PCR (containing Mg2+) buffer, 2.0 μL of dNTP with a concentration of 10 mM, 1.0 μL of an upstream primer with a concentration of 10 μM, 1.0 μL of a downstream p...

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Abstract

The invention provides an SNP locus relevant to rapid growth of crassostrea gigas. The SNP locus is located in an exon of gene LOB, and is a 341 bit starting from the 5' end of the sequence SEQ IDNO:1, and the basic group of the SNP locus is G or T; the GG type shell width of a genotype individual of the mark is remarkably larger than those of TT genotype and TG genotype individuals, the composition of an amino acid sequence is changed, and leucine is changed into phenylalanine. According to application of the SNP locus, the blindness of crassostrea gigas parent screening can be greatly reduced, and crassostrea gigas individuals with high growth speed and genetic stability can be rapidly obtained for breeding or seedling culture.

Description

technical field [0001] The invention belongs to the technical field of genetic breeding, and in particular relates to a SNP locus related to the rapid growth of oysters. Background technique [0002] The long oyster (Crassostrea gigas), also known as the Pacific oyster, is the most widely cultivated and produced marine economic shellfish in the world, and it is also an important traditional cultured species in my country. At present, while my country's long oyster aquaculture industry is developing steadily, it is also beginning to face many serious problems: with the continuous improvement of aquaculture intensification and the frequent occurrence of marine environmental pollution and red tides, the aquaculture environment of long oysters is deteriorating day by day; Breeding leads to inbreeding, and the germplasm degradation of cultured oysters is becoming more and more serious. Problems such as slow growth, low meat yield, and increased mortality have occurred, which serio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 包永波陈娜林志华王素芳
Owner ZHEJIANG WANLI UNIV
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