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Method for simultaneously and rapidly determining 32 types of free fatty acids in health-care wine by utilizing UPLC-QTof (Ultra Performance Liquid Chromatography-Quadrupole time-of-flight)

A technology for rapid determination of free fatty acids, applied in the field of detection and analysis, can solve the problems of GC/MS analysis, expensive toxic organic solvents, high processing costs, etc., achieve simple and rapid sample preparation, excellent separation effect, save time and cost Effect

Active Publication Date: 2018-09-14
ANHUI GUJING DISTILLERY +1
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

However, these methods have their own shortcomings
For example, GC methods require derivatization of fatty acids for hydrolysis and conversion to methyl esters, which is not only time-consuming, but also carries the risk of rearrangement of fatty acids during derivatization, making it impossible to determine whether the esters formed are from FFA or complete lipid complex
In addition, GC / MS analysis of low-volatility very long-chain fatty acids with high molecular weight (>C24), even after derivatization with fatty acid methyl esters (FAMEs), remains problematic
In LC / MS methods, although sample derivatization is not required, pre-run sample preparation is time-consuming and laborious, and the use of toxic organic solvents is expensive and expensive to handle

Method used

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  • Method for simultaneously and rapidly determining 32 types of free fatty acids in health-care wine by utilizing UPLC-QTof (Ultra Performance Liquid Chromatography-Quadrupole time-of-flight)
  • Method for simultaneously and rapidly determining 32 types of free fatty acids in health-care wine by utilizing UPLC-QTof (Ultra Performance Liquid Chromatography-Quadrupole time-of-flight)
  • Method for simultaneously and rapidly determining 32 types of free fatty acids in health-care wine by utilizing UPLC-QTof (Ultra Performance Liquid Chromatography-Quadrupole time-of-flight)

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Embodiment Construction

[0059] The technical solutions of the present invention will be further elaborated below in conjunction with specific embodiments.

[0060] The present embodiment measures 32 kinds of free fatty acids in certain wine according to the following steps:

[0061] 1. Pretreatment

[0062] Measure 25mL of the wine sample to be tested in a 50mL plastic centrifuge tube, use a nitrogen blower to remove ethanol, stop nitrogen blowing when the residual liquid remains 1-2ml, transfer the residual liquid after alcohol removal to a 5mL volumetric flask, and use dichloro Constant volume of methane, filtered through a 0.22μm needle filter to obtain the sample to be tested;

[0063] 2. Detection parameter setting

[0064] The detection conditions of the ultra-high performance convergence chromatograph are set as follows:

[0065] The chromatographic column is 2.1x 150mm, 1.8μm HSS C18SB chromatographic column;

[0066] The column temperature is 50°C;

[0067] The temperature of the sample...

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Abstract

The invention discloses a method for simultaneously and rapidly determining 32 types of free fatty acids in health-care wine by utilizing UPLC-QTof (Ultra Performance Liquid Chromatography-Quadrupoletime-of-flight). The method comprises the following steps: after pre-treating a wine sample to be detected, detecting by adopting an ultra performance combined phase chromatographs to obtain a chromatogram to the sample to be detected; carrying out qualitative analysis on the sample to be detected according to selected ions; then carrying out quantitative analysis on the sample to be detected according to a standard curve of the 32 types of free fatty acids. The method disclosed by the invention is simple and rapid to operate, is accurate and reliable and has good repeatability.

Description

technical field [0001] The invention relates to a method for simultaneously and quickly measuring 32 kinds of free fatty acids in health-care wine by using UPLC-QTof, which belongs to the technical field of detection and analysis. Background technique [0002] Both free fatty acids and fatty acids that make up complex lipids play some key roles in metabolism—as major metabolic fuels (storage and transport of energy), essential components of all cell membranes, and gene regulators. In addition, dietary lipids provide polyunsaturated fatty acids that are precursors to powerful locally acting metabolites such as eicosanoids. Common fatty acids from animal and plant sources are all even-numbered chains, each chain contains 16 to 24 carbon atoms, and has 0 to 6 double bonds. But there are always countless exceptions in nature, and in fact there are even odd- and even-numbered carbon fatty acids with as many as nearly 100 carbon atoms. In addition, double bonds can be in the cis...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88
CPCG01N30/88
Inventor 高家坤王银辉王银磊
Owner ANHUI GUJING DISTILLERY
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