75 results about "Quadrupole time of flight" patented technology

The invention relates to a method for detecting unknown poison by establishing a liquid chromatography-mass spectrometry database, in particular to a method used for detecting unknown poison during food poisoning. According to the method, firstly, an ultra-high performance liquid chromatography-quadrupole-time-of-flight mass spectrometry method is used for establishing a liquid chromatography-mass spectrometry database of common poison; then, a sample is subjected to supersonic extraction with methyl alcohol or acetonitrile, liquid chromatography-mass spectrometry data of an extracting solution are measured similarly and searched and compared in the liquid chromatogram-mass spectrometry database of common poison according to the retention time of the sample and mass spectrometry fragments, and the variety of the unknown poison in the sample is judged; and the unknown poisoning sample is simply extracted and directly measured and compared, and a screening result can be acquired in one hour, so that the detecting and treating time of the sample is greatly shortened, the detecting efficiency is improved, and technical support is provided for related events such as food poisoning and the like caused by unknown reasons.

A means and method are disclosed whereby ions from an ion source can be selected and transferred to a time-of-flight mass analyzer via an arrangement of multipoles in such a way that fragmented ions may be generated by collision-induced dissociation or surface-induced dissociation. First, ions from the source are collisionally cooled by a first multipole. Second, the m / z range of the ions is then selected by a second multipole (preferably a quadrupole). Third, the selected ions are allowed to collide with a "collision surface" capable of producing fragment ions. Fourth, these fragment ions are collisionally cooled in a third multipole and delivered into a TOF mass analyzer for subsequent analysis of the fragmented ions.

The present invention is directed to a simple method for absolute quantification of plasma vitellogenin from two or more different fish species such as Rainbow trout and Atlantic salmon, or Atlantic cod and haddock. In the case of Rainbow trout and Atlantic salmon, plasma samples obtained from control and β-estradiol induced fish were digested with trypsin. A characteristic ‘signature peptide’ was selected and analyzed by high performance liquid chromatography coupled to an electrospray quadrupole-time-of-flight tandem mass spectrometer, using a deuterated homologue peptide as an internal standard. The hybrid tandem mass spectrometer was operated in a ‘pseudo’ selected reaction monitoring mode by which three diagnostic product ions were monitored for identification and quantification purposes. The reproducibility (coefficient of variation ˜5%) and sensitivity (limit of quantification of 0.009 mg / mL) achieved by this simple assay allow it to be considered as an alternative to immunological assays. In the case of Atlantic cod and haddock, the amino acid sequence of the vitellogenin protein has not yet been determined, but, the Atlantic cod vitellogenin has been characterized using a ‘bottom-up’ mass spectrometric approach. Vitellogenin synthesis was induced ‘in vivo’ with β-Estradiol, and subjected to trypsin digestion for characterization by matrix-assisted laser desorption / ionization-Quadrupole-Time-of-flight tandem mass spectrometry. A peptide mass fingerprint was obtained and ‘de novo’ sequencing of the most abundant tryptic peptides was performed by low energy collision induced dissociation-tandem mass spectrometry. Thus, the sequences of various tryptic peptides have been elucidated. It has also been determined that Atlantic cod vitellogenin shares a series of common peptides with the two different known vitellogenin sequences of Haddock, a closely related species. There are also disclosed novel isolated signature peptides, namely Thr-Tyr-Phe-Ala-Gly-Ala-Ala-Ala-Asp-Val-Leu-Glu-Val-Gly-Val-Arg, Asp Leu Gly Leu Ala Tyr Thr Glu Lys, Phe Phe Gly Gln Glu Ile Ala Asn Ile Asp Lys, Glu Ile Val Leu Leu Gly Tyr Gly Thr Met Ile Ser Lys and Tyr Glu Ser Phe Ala Val Ala Arg.

An application method of a serum lipid biomarker in NSCLC early diagnosis includes the following steps: collecting serum samples of NSCLC patients, patients with benign lung lesions and normal people;pretreating the serum samples, and detecting lipid metabolism markers in each serum sample by utilizing a method of ultra-high performance liquid chromatography-quadrupole-time of flight mass spectrometry; screening NSCLC-related differential lipid metabolism markers through a method of multivariate pattern recognition analysis; screening out key metabolic pathways with highest correlation with the lipid metabolites through KEGG analysis and metabolic pathway analysis of NSCLC differential lipid metabolites; performing network analysis of "gene-enzyme-reaction-metabolite" of the NSCLC differential lipid metabolites, to obtain a network graph of the NSCLC differential lipid metabolites; and screening and obtaining the serum lipid biomarker for the NSCLC early diagnosis through integrationof the screening of the NSCLC differential lipid metabolism markers and analysis results of the metabolic pathways.

The invention belongs to the field of analytic chemistry, resveratrol from polygonum cuspidatum and resveratrol from grape vine are taken as research objects, a liquid chromatography-mass spectrometry technology (LC-QTOF) with high sensitivity and high resolution is adopted for providing more characteristics of compound structural information, the difference of micro components in resveratrol samples is researched, four marking compounds which can distinguish resveratrol of different sources are found, the difference of the micro components in the resveratrol from polygonum cuspidatum and resveratrol from grape vine is further confirmed, a reference method is provided for distinguishing resveratrol samples of different raw material sources, and great significance for identifying the real property of the natural healthcare food, namely, resveratrol, is achieved, and meanwhile the technical method can be also widely popularized and applied to real property detection and identification standard of imported and exported agriculture products and biologic raw materials, technical demonstration for identification on the real property of the natural product raw material is provided, and establishment of reference standards is achieved.

A miniature mass spectrometer is disclosed comprising an atmospheric pressure ionisation source and a first vacuum chamber having an atmospheric pressure sampling orifice or capillary, a second vacuum chamber located downstream of the first vacuum chamber and a third vacuum chamber located downstream of the second vacuum chamber. An ion detector is located in the third vacuum chamber. A first RF ion guide is located within the first vacuum chamber and a second RF ion guide is located within the second vacuum chamber. The ion path length from the atmospheric pressure sampling orifice or capillary to an ion detecting surface of the ion detector is ≦400 mm. The mass spectrometer further comprises a tandem quadrupole mass analyser, a 3D ion trap mass analyser, a 2D or linear ion trap mass analyser, a Time of Flight mass analyser, a quadrupole-Time of Flight mass analyser or an electrostatic mass analyser arranged in the third vacuum chamber. The product of the pressure P1 in the vicinity of the first RF ion guide and the length L1 of the first RF ion guide is in the range 10-100 mbar-cm and the product of the pressure P2 in the vicinity of the second RF ion guide and the length L2 of the second RF ion guide is in the range 0.05-0.3 mbar-cm.

The invention discloses a method for simultaneously and rapidly determining 32 types of free fatty acids in health-care wine by utilizing UPLC-QTof (Ultra Performance Liquid Chromatography-Quadrupoletime-of-flight). The method comprises the following steps: after pre-treating a wine sample to be detected, detecting by adopting an ultra performance combined phase chromatographs to obtain a chromatogram to the sample to be detected; carrying out qualitative analysis on the sample to be detected according to selected ions; then carrying out quantitative analysis on the sample to be detected according to a standard curve of the 32 types of free fatty acids. The method disclosed by the invention is simple and rapid to operate, is accurate and reliable and has good repeatability.