75 results about "Quadrupole time of flight" patented technology

The invention relates to a method for detecting unknown poison by establishing a liquid chromatography-mass spectrometry database, in particular to a method used for detecting unknown poison during food poisoning. According to the method, firstly, an ultra-high performance liquid chromatography-quadrupole-time-of-flight mass spectrometry method is used for establishing a liquid chromatography-mass spectrometry database of common poison; then, a sample is subjected to supersonic extraction with methyl alcohol or acetonitrile, liquid chromatography-mass spectrometry data of an extracting solution are measured similarly and searched and compared in the liquid chromatogram-mass spectrometry database of common poison according to the retention time of the sample and mass spectrometry fragments, and the variety of the unknown poison in the sample is judged; and the unknown poisoning sample is simply extracted and directly measured and compared, and a screening result can be acquired in one hour, so that the detecting and treating time of the sample is greatly shortened, the detecting efficiency is improved, and technical support is provided for related events such as food poisoning and the like caused by unknown reasons.

A means and method are disclosed whereby ions from an ion source can be selected and transferred to a time-of-flight mass analyzer via an arrangement of multipoles in such a way that fragmented ions may be generated by collision-induced dissociation or surface-induced dissociation. First, ions from the source are collisionally cooled by a first multipole. Second, the m / z range of the ions is then selected by a second multipole (preferably a quadrupole). Third, the selected ions are allowed to collide with a "collision surface" capable of producing fragment ions. Fourth, these fragment ions are collisionally cooled in a third multipole and delivered into a TOF mass analyzer for subsequent analysis of the fragmented ions.

The invention discloses an anti-inflammation active component group of a stomatitis clearing preparation and an analysis method. Fingerprint characteristic spectra of the component group are constructed and are applied to detecting the quality of the stomatitis clearing preparation. The analysis method which is a quality detecting method particularly includes detecting the quality of the stomatitis clearing preparation by the aid of a detection index which is anti-inflammation efficacy; uniformly designing difference samples with different component proportions; acquiring fingerprint characteristic data of the difference samples by the aid of UFLC-Q-TOF-MS / MS (ultrafast liquid chromatography-quadrupole-time of flight-mass spectrometry / mass spectrometry) technologies; acquiring anti-inflammation activity data of the preparation. The relevance between chemical components of the difference samples and the efficacy is comprehensively analyzed by the aid of the mathematical statistics method, accordingly, 29 anti-inflammation efficacy active components can be determined, and the fingerprint characteristic spectra of the component group can be constructed. Compared with the prior art, the anti-inflammation active component group and the analysis method have the advantages that the active component group is analyzed on the basis of the relevance between the spectra and the efficacy, accordingly, the quality of raw medicinal materials, semi-finished products and finished products can be directly, objectively, scientifically and comprehensively monitored in production procedures, and the effectiveness and the quality consistency of the products can be guaranteed.

The invention belongs to the field of food inspection, relates to an assay determination method for endocrine disruptors in food, and particularly relates to a method for analyzing and determining a plurality of endocrine disruptors in milk powder and liquid milk. The method comprises the steps of dissolving a sample with water, adding an organic solvent mixable with water, extracting a to-be-detected material by ultrasonic, adding a sodium salt to make the organic solvent separated from a water phase to realize liquid-liquid extraction, taking an organic solvent containing quantitative to-be-detected material, purifying by extraction with a solid phase filled with C 18 materials, deriving by dansyl chloride, and analyzing and detecting four types of 26 endocrine disruptors in the food by using ultra-high performance liquid chromatography-quadrupole-time of flight-mass spectrometry at the same time. The method can overcome the disadvantages that a conventional technology cannot realize simultaneous analysis of the plurality of the endocrine disruptors in the food by once chromatographic sample injection, can increase sensitivity of the quadrupole-time of the flight-mass spectrometry, and realize accurate analysis of the endocrine disruptors in the food by using the quadrupole-time of the flight-mass spectrometry.

The invention discloses a screening method for 110 types of medicines in feed, which specifically includes the following steps: preparation of standard work solution, pre-analysis treatment of sample to be assayed, creation of database and qualitative analysis. By utilizing the high-sensitivity MSMS (Tandem Mass Spectrometry) function of a liquid chromatography-tandem quadrupole time-of-flight mass spectrometer, the screening method can accurately determine parent ions and daughter ions in medicines and rapidly determine added medicine ingredients, moreover, an assay method for a variety of antibacterial medicines in feed is also established, and for a variety of added antibacterial medicines in the feed industry in China, the screening method has a highly efficient, accurate and precise assay measure, and is at the advanced level in china at present. The screening method established by the invention greatly solves the phenomenon of arbitrary addition in the feed industry, the more precise and accurate assay method is perfected for the inspection department, more time is saved, most of experimental consumables are saved, and the invention makes an immeasurable contribution to food safety and human healthy life.

A miniature mass spectrometer is disclosed comprising an atmospheric pressure ionisation source, a first vacuum chamber having an atmospheric pressure sampling orifice or capillary, a second vacuum chamber located downstream of the first vacuum chamber and a third vacuum chamber located downstream of the second vacuum chamber. A first vacuum pump is arranged and adapted to pump the first vacuum chamber, wherein the first vacuum pump is arranged and adapted to maintain the first vacuum chamber at a pressure <10 mbar. A first RF ion guide is located within the first vacuum chamber and an ion detector is located in the third vacuum chamber. The ion path length from the atmospheric pressure sampling orifice or capillary to an ion detecting surface of the ion detector is ≦400 mm. The mass spectrometer further comprises a tandem quadrupole mass analyser, a 3D ion trap mass analyser, a 2D or linear ion trap mass analyser, a Time of Flight mass analyser, a quadrupole-Time of Flight mass analyser or an electrostatic mass analyser arranged in the third vacuum chamber. A split flow turbomolecular vacuum pump comprising an intermediate or interstage port is connected to the second vacuum chamber and a high vacuum (“HV”) port is connected to the third vacuum chamber. The first vacuum pump is also arranged and adapted to act as a backing vacuum pump to the split flow turbomolecular vacuum pump and the first vacuum pump has a maximum pumping speed ≦10 m3 / hr (2.78 L / s).

The present invention is directed to a simple method for absolute quantification of plasma vitellogenin from two or more different fish species such as Rainbow trout and Atlantic salmon, or Atlantic cod and haddock. In the case of Rainbow trout and Atlantic salmon, plasma samples obtained from control and β-estradiol induced fish were digested with trypsin. A characteristic ‘signature peptide’ was selected and analyzed by high performance liquid chromatography coupled to an electrospray quadrupole-time-of-flight tandem mass spectrometer, using a deuterated homologue peptide as an internal standard. The hybrid tandem mass spectrometer was operated in a ‘pseudo’ selected reaction monitoring mode by which three diagnostic product ions were monitored for identification and quantification purposes. The reproducibility (coefficient of variation ˜5%) and sensitivity (limit of quantification of 0.009 mg / mL) achieved by this simple assay allow it to be considered as an alternative to immunological assays. In the case of Atlantic cod and haddock, the amino acid sequence of the vitellogenin protein has not yet been determined, but, the Atlantic cod vitellogenin has been characterized using a ‘bottom-up’ mass spectrometric approach. Vitellogenin synthesis was induced ‘in vivo’ with β-Estradiol, and subjected to trypsin digestion for characterization by matrix-assisted laser desorption / ionization-Quadrupole-Time-of-flight tandem mass spectrometry. A peptide mass fingerprint was obtained and ‘de novo’ sequencing of the most abundant tryptic peptides was performed by low energy collision induced dissociation-tandem mass spectrometry. Thus, the sequences of various tryptic peptides have been elucidated. It has also been determined that Atlantic cod vitellogenin shares a series of common peptides with the two different known vitellogenin sequences of Haddock, a closely related species. There are also disclosed novel isolated signature peptides, namely Thr-Tyr-Phe-Ala-Gly-Ala-Ala-Ala-Asp-Val-Leu-Glu-Val-Gly-Val-Arg, Asp Leu Gly Leu Ala Tyr Thr Glu Lys, Phe Phe Gly Gln Glu Ile Ala Asn Ile Asp Lys, Glu Ile Val Leu Leu Gly Tyr Gly Thr Met Ile Ser Lys and Tyr Glu Ser Phe Ala Val Ala Arg.

The invention relates to a simultaneous screening and detection method of a plurality of types of veterinary drug residues in solid animal-derived foods. The simultaneous screening and detection method comprises the following steps: after taking and crushing a sample to be detected, adding an acetonitrile acetate solution and a Na2EDTA buffering solution into the sample to be detected; meanwhile, adding anhydrous sodium sulfate and sodium chloride, and homogenizing and extracting; adding an adsorbent containing C18 and NH2 into extracting-obtained liquid supernatant to carry out purification; then determining by using high performance liquid chromatography-quadrupole-time-of-flight mass spectrometry. The screening method provided by the invention can be used for simultaneously screening and detecting the plurality of types of veterinary drug residues in the animal-derived foods, has the characteristics of rapidness, simplicity and convenience, flexibility, accuracy and the like, can be used for carrying out an accurate qualitative analysis on target veterinary drugs in the sample, and also can be used for carrying out quantitative detection. The method is applicable to rapid detection of the plurality of types of veterinary drug residues in batch samples. Aiming at the solid animal-derived foods, the impact on the target veterinary drugs by sample substrates is reduced, and the extraction effect is good and the recycling rate is high.

An application method of a serum lipid biomarker in NSCLC early diagnosis includes the following steps: collecting serum samples of NSCLC patients, patients with benign lung lesions and normal people;pretreating the serum samples, and detecting lipid metabolism markers in each serum sample by utilizing a method of ultra-high performance liquid chromatography-quadrupole-time of flight mass spectrometry; screening NSCLC-related differential lipid metabolism markers through a method of multivariate pattern recognition analysis; screening out key metabolic pathways with highest correlation with the lipid metabolites through KEGG analysis and metabolic pathway analysis of NSCLC differential lipid metabolites; performing network analysis of "gene-enzyme-reaction-metabolite" of the NSCLC differential lipid metabolites, to obtain a network graph of the NSCLC differential lipid metabolites; and screening and obtaining the serum lipid biomarker for the NSCLC early diagnosis through integrationof the screening of the NSCLC differential lipid metabolism markers and analysis results of the metabolic pathways.

The invention provides a mass spectrometry identification method of nucleophosmin variable spliceosome and a gastric cancer diagnostic reagent kit. The method is combined by a Glu-C enzyme cutting method and a Q-TOF (quadrupole time-of flight) mass spectra method, and can be applied to the clinical diagnosis and treatment evaluation of the gastric cancer; and by the method disclosed by the invention, the rapid and reliable identification result can be acquired as compared with the traditional methods.

The invention discloses a method for creating a multi-index quantitative fingerprint spectrum for food retention-removing and cough-relieving oral solution for children, which includes the following steps: high-performance liquid chromatography (HPLC)-diode array detector-electrospray ionization-quadrupole-time of flight mass spectrometry combination is adopted to detect chemical information characteristic peaks of a variety of common chemical substances in multiple batches of food retention-removing and cough-relieving oral solution for children, and according to the detected chromatographic peaks of a variety of the common chemical substances in the multiple batches of retention-removing and cough-relieving oral solution for children in combination with multi-index ingredient content determination, a fingerprint spectrum of the food retention-removing and cough-relieving oral solution for children is created. The method adopts the HPLC-DAD-ESI-Q-TOF / MS technique to analyze and identify chemical ingredients in the food retention-removing and cough-relieving oral solution for children, quality control indexes are chosen in reference to the principle of concerted application of Chinese herbal medicines and by referring to individual herb quality control indexes in one volume of 2015 edition of Chinese Pharmacopoeia and an identification result of each common peak, an HPLC multi-index quantitative fingerprint spectrum is created on the basis, and thereby a relatively comprehensive method and technical support are provided for the research on the evaluation of the overall quality of the food retention-removing and cough-relieving oral solution for children.

A gas chromatography-quadrupole time-of-flight mass spectrometry / flame ionization detection method of flavor components in cigarette mainstream smoke comprises the following steps: collecting the flavor components in the cigarette mainstream smoke through adopting a Cambridge filter, extracting the flavor components with dichloromethane or n-hexane, taking the obtained extract liquid, analyzing the extract liquid, and allowing the obtained sample to undergo chromatographic column separation and to enter a quadrupole time-of-flight mass spectrometer (QTOF MS) and a flame ionization detector (FID) for determination in order to realize accurate qualitative diagnosis and quantification of the flavor components in cigarette mainstream smoke. The gas chromatography-quadrupole time-of-flight mass spectrometry / flame ionization detection method is applied to determination of the flavor components in cigarette mainstream smoke for the first time, and a QTOF MS spectrogram and an FID spectrogram can be simultaneously obtained only through one-shot sample introduction; and the components of a complex sample can be accurately characterized through the QTOF MS, a sample with very wide concentration range can be accurately quantified, and the retention times of the two spectrograms are completely same, so accurate qualitative diagnosis and quantification of the flavor components in cigarette mainstream smoke can be realized.

The invention relates to detection and preparation methods of azilsartan medoxomil tetramer impurities. The detection method comprises the step of performing gradient elution by using an Agilent Eclipse XDB C18 chromatographic column and a mixed mobile phase formed by ammonium acetate buffer salt with the pH value of 3.5 as a mobile phase A and acetonitrile as a mobile phase B according to the following conditions: the volume ratio of the mobile phase A to the mobile phase B from 0 to 8min is 55:45, the volume ratio of the mobile phase A to the mobile phase B from 8min to 18min is 55:45 to 40:60, the volume of the mobile phase A to the mobile phase B from 18min to 28min is 40:60 to 10:90, and the volume ratio of the mobile phase A to the mobile phase B from 28min to 60min is 10:90; and a detector is a quadrupole time of flight mass spectrometry detector (Q-TOF).

The invention belongs to the field of analytic chemistry, resveratrol from polygonum cuspidatum and resveratrol from grape vine are taken as research objects, a liquid chromatography-mass spectrometry technology (LC-QTOF) with high sensitivity and high resolution is adopted for providing more characteristics of compound structural information, the difference of micro components in resveratrol samples is researched, four marking compounds which can distinguish resveratrol of different sources are found, the difference of the micro components in the resveratrol from polygonum cuspidatum and resveratrol from grape vine is further confirmed, a reference method is provided for distinguishing resveratrol samples of different raw material sources, and great significance for identifying the real property of the natural healthcare food, namely, resveratrol, is achieved, and meanwhile the technical method can be also widely popularized and applied to real property detection and identification standard of imported and exported agriculture products and biologic raw materials, technical demonstration for identification on the real property of the natural product raw material is provided, and establishment of reference standards is achieved.

The invention provides LC-Q-TOF / MS (liquid chromatography-quadrupole-time of flight / mass spectrometry) technology for detecting 544 kinds of pesticide residues in melons and fruit. In a TOF / MS mode, LC-Q-TOF / MS is used for respectively determining the retention time of each pesticide standard substance under specified chromatography-mass spectrometry conditions, determining the ionization forms and chemical formula of the compound under the condition of ESI (electron spray ionization) and obtaining the precise mass number of the parent ion of each compound, thus forming a TOF / MS database. In a Q-TOF / MS mode, the fragment ion mass spectrum of each pesticide standard substance under 3-5 different collision energy is respectively collected and the collected information is imported into PCDL software, thus forming a Q-TOF / MS database. Whether samples contain pesticide residues is determined by comparing the retention time and primary and secondary mass spectrometry information of melon and fruit samples, secondary confirmation is carried out on the compounds with higher primary scores, and detection of related pesticide residues is confirmed if the secondary scores are higher. The technology has the advantages of rapidness, high throughput, high precision, high reliability, and the like and can be used for precisely screening pesticides in the melons and fruit.

The invention discloses a LC-Q-TOF (liquid chromatography quadrupole time-of-flight) detection method for the fipronil in an egg and an egg product. The method comprises the following steps of: sequentially adding a 0.1% formic acid acetonitrile solution, the sodium chloride, and the anhydrous magnesium sulfate to the egg and the egg product to be detected; vortexing, mixing, and centrifuging to obtain the supernatant liquid; performing supercritical CO<2> extraction on the supernatant liquid; adding the 0.1% formic acid acetonitrile solution for constant volume after the extract liquid is dried at room temperature under nitrogen; and injecting the liquid to be detected after the constant volume into LC-Q-TOF for detection. According to the LC-Q-TOF detection method for the fipronil in theegg and the egg product, the mass spectrum of the liquid to be detected is obtained by LC-Q-TOF, and the qualitative analysis of components in the liquid to be detected is realized, thereby screeningwhether the egg and the egg product to be detected contain the fipronil. At the same time, according to a chromatogram, the concentration of the fipronil-containing in the egg and the egg product tobe detected is calculated. The detection limit of the detection method is 0.4ug / Kg, which is much better than other detection methods. The detection method not only can accurately screen the qualitative fipronil in the egg and the egg product to be detected, but also accurately quantify the fipronil, which is beneficial for the detection of the fipronil in the egg and the egg product.

Disclosed is a gas chromatography-quadrupole time-of-flight mass spectrometry / flame ionization detection method for tobacco essence perfume. The method includes subjecting the tobacco essence perfume to organic solvent extraction and centrifugation, taking an organic solvent layer to analyze, regulating tail-end distribution of a chromatographic column through a capillary flow technology after the sample is subjected to chromatographic column separation, and determining by a QTOF MS (quadrupole time-of-flight mass spectrometer) and FID (flame ionization detector) to achieve accurate qualitativeness and quantitativeness of the tobacco essence perfume. The gas chromatography-quadrupole time-of-flight mass spectrometry / flame ionization detection method is put forward for the first time and is applied to determination of the tobacco essence perfume, QTOF MS and FID spectrograms can be obtained simultaneously only by one-needle sample injection, QTOF MS can determine components of complicated samples accurately, the FID is capable of qualifying the sample with wide concentration range accurately, and the retention time of the QTOF MS spectrogram and the retention time of the FID spectrogram are the same, so that accurate qualitativeness and quantitativeness of complicated tobacco essence perfume samples can be achieved.

The invention discloses a detection method of carbon tetrafluoride in a column type circuit breaker for a 1100kV filter. The detection method comprises the following steps: 1) using gas in the columntype circuit breaker for the 1100kV filter as a to-be-detected sample; injecting the to-be-detected sample into a gas chromatography-quadrupole time-of-flight tandem gas chromatograph-mass spectrometer to obtain the peak area of CF4 of the to-be-detected sample in an ionic chromatogram; 2) comparing the peak area obtained in the step 1) with a standard curve of a CF4 standard product according toa chromatographic analysis external standard method so as to obtain content of the detected CF4 in the column-type circuit breaker for the 1100kV filter. The method is strong in pertinency, high in sensitivity and is suitable for detecting the content of the CF4 in the column-type circuit breaker for the 1100kV filter; moreover, the quantitative lower limit is up to 0.13 micro liter / liter.

The invention discloses a method for analyzing phenolic compounds in water, used for qualitatively or quantitatively analyzing the phenolic compounds in a water sample. The method comprises the following steps of 1, producing a standard curve; 2, preparing sample liquid to be detected: preprocessing a surface water sample to be detected to acquire the sample liquid to be detected; 3, detecting andanalyzing the sample liquid to be detected by using gas chromatography-low energy electron ionization quadrupole time-of-flight mass spectrometry (GC-le EI-QTOF); and 4, reviewing the sample that isdetected to be positive, namely that includes the phenolic compounds, by using gas chromatography-triple quadrupole mass spectrometry (GC-QQQ). The method provided by the invention is high in resolution, high in sensitivity, and capable of accurately determining the nature of a target, and excluding interferent of which a mass-to-charge ratio is close to that of the target.

The invention provides a method for identifying intracellular small molecule metabolites. The qualitative analysis of the metabolites is performed according to an exact mass number and chromatogram matching, and the metabolite is relatively quantified according to an abundance of the chromatogram. The procedure of the analysis method is as follows: washing cultured adherent cells with PBS quicklyand then quenched with liquid nitrogen; then adding an extract liquor to extract all metabolites in the cells; searching KEGG and METLIN databases to obtain parameters of the metabolic pathway main metabolites such as the ion pair and collision energy and the like, and establishing a detection and analysis method of the small molecule metabolites; and performing qualitative and quantitative analysis on the intracellular metabolites by using tandem quadrupole linear ion trap high performance mass spectrometry (Q-Trap) combined with quadrupole-time of flight mass spectrometry (Q-TOF). The methodfor identifying the intracellular small molecule metabolites can be used for qualitative and quantitative analysis of metabolites in cell samples; the range of the identified metabolites detected bythe method is wider; and the linear range of the metabolites is wider; therefore, the problem that the qualitative analysis of the metabolites in the prior art is difficult and the analysis requires alarge number of standards for comparison is effectively solved.

The invention relates to a method for qualitatively and quantitatively analyzing non-volatile secondary metabolite chemical components of heartleaf houttuynia herb wall-broken medicinal slices. The method includes the steps: S1 pretreating the heartleaf houttuynia herb wall-broken medicinal slices to prepare sample solution; S2 taking a reference substance as a contrast, comparing pyrolysis fragmentation information with corresponding databases by the aid of a UHPLC-Q-TOF / MS (ultra-high-performance liquid chromatography quadrupole time-of-flight mass spectrometry) technique, and qualitativelyand quantitatively analyzing non-volatile secondary metabolite chemical components in a sample. A moving phase A is formic acid water solution, and a moving phase B is formic acid acetonitrile solution. According to the method, the non-volatile secondary metabolite chemical components of the heartleaf houttuynia herb wall-broken medicinal slices are qualitatively and quantitatively analyzed by theaid of the UHPLC-Q-TOF / MS technique, 36 non-volatile secondary metabolite products such as phenolic acids, flavonoid and alkaloids are detected, and the method has the advantages of high speed, highspecificity, low detection limit, high qualitative and quantitative ability and the like.

The invention discloses a method for simultaneously and rapidly determining 32 types of free fatty acids in health-care wine by utilizing UPLC-QTof (Ultra Performance Liquid Chromatography-Quadrupoletime-of-flight). The method comprises the following steps: after pre-treating a wine sample to be detected, detecting by adopting an ultra performance combined phase chromatographs to obtain a chromatogram to the sample to be detected; carrying out qualitative analysis on the sample to be detected according to selected ions; then carrying out quantitative analysis on the sample to be detected according to a standard curve of the 32 types of free fatty acids. The method disclosed by the invention is simple and rapid to operate, is accurate and reliable and has good repeatability.

The invention discloses a qualitative method of phenolic compounds in tea seeds. The method is characterized in that an ultra-high performance liquid chromatography-quadrupole-time of flight mass spectrometer method is adopted for identifying; tea seed powder is pretreated, and is detected through the ultra-high performance liquid chromatography-quadrupole-time of flight mass spectrometer to obtain a total ion chromatogram and extracted feature ion chromatogram; elements and accurate molecular weight of a compound corresponding to the ion are calculated by Q-TOF qualitative software; the unknown compound is initially identified according to element composition and accurate molecular weight, cracking the known compound by collision-induction; and the variety of phenolic compounds in tea seeds can be deducted according to the ion cracking situation. Compared with an existing phenolic compound qualitative method, the method has the advantages of no standard product, high analyzing sensitivity and good selectivity.

The invention relates to an ESI-Q-TOF MS (Electrospray Ionization-Quadrupole-Time-of-Flight Mass Spectrometry) analyzing method of an edible oil sample, and particularly relates to a method for qualitatively or quantitatively analyzing the sterols compounds contained in the edible oil sample by combining an N-alkyl pyridine isotope derivatization method with ESI-Q-TOF MS analysis. The method comprises the following steps of: carrying out sample pretreatment on a target analyte; reacting the alcoholic hydroxyl of the target analyte with pyridine or deuterated pyridine; and diluting a marked sample solution for the ESI-Q-TOF MS analysis. The method disclosed by the invention has the advantages of fastness, high efficiency and flexibility and lays a foundation for further measuring and identifying possible unqualified edible oil by identifying phytosterin and zoosterol which are contained in edible oil.

The invention relates to the technical field of plant extraction and provides a method for extracting and detecting saponin active components in ginseng leaves, thereby solving problems of long extraction time, low extraction efficiency, and tedious operation process of the traditional extraction method. The method comprises the following steps: step one, carrying out smashing and sieving; step two, adding an adsorbent and carrying out grinding; step three, transferring ground powder into a solid phase extraction void column, an upper sieve plate and a lower sieve plate carry out compression,carrying out elution and centrifugation to obtain a supernatant; and step four, transferring the supernatant and analyzing a target compound by using an ultra-high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry. According to the invention, with the matrix solid-phase dispersion extraction technique and the metal organic framework as an adsorbent, the saponin active components in ginseng leaves can be extracted and enriched simultaneously and effectively, so that the environmental pollution is low; for a trace amount of ginseng leaf samples, the high extraction efficiency is realized; and with the ultra-high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry joint technology as a detection way for a target analyte, the detection sensitivity is high; and the detection is simple, fast and effective.

A rapid detection method of tobacco leaf volatile components is characterized in that the method adopts dichloromethane for extraction of tobacco leaves, a sample after extraction is separated by a chromatographic column, a tail end shunt of the chromatographic column is regulated by using a micro plate flow path control technology, at the same time, the shunt enters a quadrupole time-of-flight mass spectrometer (QTOF MS) and a flame ionization detector (FID) and is determined, accurate qualitation and quantitation of the tobacco leaf volatile components is achieved, the whole process has the advantages of simple operation and short use time, and mass and automatic processing of the sample is achieved. The process can be completed by only requiring a simple solvent for extraction, a small amount of an organic solvent is consumed, the whole process has no second-order reaction problem of the tobacco leaf volatile components, and actual conditions of the sample is really reflected; an extract liquid is detected by using gas chromatography-quadrupole time-of-flight mass spectrometry / flame ionization detection for a first time, only one-needle sample injection is needed, and a QTOF MS spectrogram and an FID spectrogram are obtained, wherein QTOF MS can perform accurate quantitation of components of complex samples while the FID can perform accurate quantification of samples with wide concentration scope, and the retaining time of the QTOF MS spectrogram and the retaining time of the FID spectrogram are just the same.

The invention discloses a method for detecting the in-vivo direct acting component in a periploca forrestii schltr medicinal material. An UHPLC-Q-TOF-MS (Ultra-High Performance Liquid Chromatography-Quadrupole-Time Of Flight-Mass Spectrometry) technology is adopted; a serum fingerprint chromatogram and a blank rat serum fingerprint chromatogram are compared after a periploca forrestii schltr extractive and a rat oral periploca forrestii schltr extractive; the migration component in rat blood after the oral periploca forrestii schltr extractive is determined; the in-vivo direct acting componentin the periploca forrestii schltr medicinal material is definite; and thus, reference is provided for effect material basis that periploca forrestii schltr plays a pharmacological role and quality control.

The invention relates to a method for determining the quantity of diquat or paraquat residual in food. The method comprises the steps of pretreating a sample: firstly extracting the diquat or the paraquat in the food sample by using a methanol aqueous solution; purifying the extract by using C18 and PSA (Primary Secondary Amine) dispersive solid-phase extraction agents; and finally by combining ahigh performance liquid chromatography with a quadrupole time-of-flight mass spectrometry, realizing qualitative and quantitative determination of the diquat or the paraquat residual in the food through the accurate mass number of compound ions. The method disclosed by the invention has the beneficial effects that the matrix interference in the detection of the diquat or the paraquat residual in the food is effectively reduced; the recovery rate of the diquat or the paraquat can be up to 80.8%-97.3%; the relative standard deviation is 3.5%-7.8%; the limit of quantification is 10mug / kg; the method has the advantages of being simple and convenient to operate, fast, accurate, high in sensitivity and good in repeatability; and the method is a supplement and an improvement on a conventional QuEChERS method.