Method for extracting and detecting saponin active components in ginseng leaves
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A technology of active ingredients and detection methods, which is applied in the field of extraction and detection of saponin active ingredients in ginseng leaves, can solve the problems of low reproducibility and accuracy, and achieve the effects of low environmental pollution, high extraction efficiency, and high detection sensitivity
Active Publication Date: 2019-05-03
HANGZHOU NORMAL UNIVERSITY
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Embodiment 1
[0050] (1) Preparation of reference substance mixed solution: Weigh ginsenoside Rg1, ginsenoside Re, ginsenoside Rg2, ginsenoside Rb1, and ginsenoside Rd reference substance in appropriate amounts, accurately weigh them, and place them in brown measuring bottles and add them separately Methanol is formulated into a reference solution containing 1000μg per 1mL, and stored at 4℃ for later use; Figure 4 It is a liquid chromatogram of a mixed standard reference solution with a concentration of 100μg / mL;
[0051] (2) Preparation of metal-organic framework MOF-808: Weigh out 55 mg of trimellitic acid and 80 mg of zirconyl chloride octahydrate, and dissolve them in 10 mL of N,N-dimethylformamide and 10 mL of formic acid mixed solution. , The solution was transferred to a glass bottle and sealed, and reacted at 100°C for seven days. After the reaction, it was cooled and centrifuged at 13000 rpm for 5 min. After centrifugation, the upper clear liquid was removed, and the white gelatinou...
Embodiment 2
[0064] The difference between Example 2 and Example 1 is that the adsorbent is carbon nanotubes, and the rest of the process steps are completely the same.
[0065] In this example, after analysis and determination, the content of ginsenoside Rg2 in the ginseng leaf sample powder is 0.4012mg / g, the content of ginsenoside Rg1 is 1.235mg / g, the content of ginsenoside Re is 2.014mg / g, ginsenoside The content of Rd is 0.4859mg / g, and the content of ginsenoside Rb1 is 1.1250mg / g.
Embodiment 3
[0067] The difference between Example 3 and Example 1 is that the adsorbent is graphene, and the rest of the process steps are completely the same.
[0068] In this example, after analysis and determination, the content of ginsenoside Rg2 in the ginseng leaf sample powder is 0.4275mg / g, the content of ginsenoside Rg1 is 1.2450mg / g, the content of ginsenoside Re is 2.0856mg / g, ginsenoside The content of Rd is 0.5456mg / g, and the content of ginsenoside Rb1 is 1.3403mg / g.
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Abstract
The invention relates to the technical field of plant extraction and provides a method for extracting and detecting saponin active components in ginseng leaves, thereby solving problems of long extraction time, low extraction efficiency, and tedious operation process of the traditional extraction method. The method comprises the following steps: step one, carrying out smashing and sieving; step two, adding an adsorbent and carrying out grinding; step three, transferring ground powder into a solid phase extraction void column, an upper sieve plate and a lower sieve plate carry out compression,carrying out elution and centrifugation to obtain a supernatant; and step four, transferring the supernatant and analyzing a target compound by using an ultra-high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry. According to the invention, with the matrix solid-phase dispersion extraction technique and the metal organic framework as an adsorbent, the saponin active components in ginseng leaves can be extracted and enriched simultaneously and effectively, so that the environmental pollution is low; for a trace amount of ginseng leaf samples, the high extraction efficiency is realized; and with the ultra-high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry joint technology as a detection way for a target analyte, the detection sensitivity is high; and the detection is simple, fast and effective.
Description
Technical field [0001] The invention relates to the technical field of plant extraction, in particular to a method for extracting and detecting saponins active ingredients in ginseng leaves. Background technique [0002] Different types and parts of ginseng are considered to have different medicinal properties. Studies have shown that ginseng can relieve or improve immune, endocrine, cardiovascular and central nervous system diseases. In the Chinese Pharmacopoeia, ginseng leaves and ginseng, which are taken from the leaves and roots of ginseng, can be used as Chinese medicine. It is worth mentioning that Asians have used plants or herbs for nearly 2,000 years to deal with some common diseases. Researchers have also conducted extensive studies on the active ingredients of ginseng, such as ginsenosides, polyacetylenes, acid polysaccharides, insulin-like acid peptides, antioxidant aromatic compounds, especially ginsenosides, which have many biological activities. Including anti-di...
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