Application method of serum lipid biomarker in NSCLC early diagnosis

A biomarker, early diagnosis technology, applied in disease diagnosis, biological material analysis, measurement devices, etc., can solve the problems of inability to analyze thermally unstable metabolites, limited research scope, etc., to shorten the screening time, enhance the Reliable and accurate results

Active Publication Date: 2018-10-19
HUZHOU CENT HOSPITAL
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  • Claims
  • Application Information

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Problems solved by technology

Gas chromatography / mass spectrometry (GC-MS) has high sensitivity, but its research scope is limited to the analysis of volatile substances, and cannot analyze metabolites with thermal instability and large molecular weight

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  • Application method of serum lipid biomarker in NSCLC early diagnosis
  • Application method of serum lipid biomarker in NSCLC early diagnosis
  • Application method of serum lipid biomarker in NSCLC early diagnosis

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Embodiment Construction

[0044] The present invention will be further described below in conjunction with specific examples, but the present invention is not limited to these specific implementations. Those skilled in the art will realize that the present invention covers all alternatives, modifications and equivalents as may be included within the scope of the claims.

[0045] see figure 1 The application method of serum lipid biomarkers of the present invention in the early diagnosis of NSCLC includes two parts, specifically as follows:

[0046] 1. Non-target NSCLC lipidomics detection and analysis based on UHPLC-Q-TOF / MS technology

[0047] (1) Instruments and reagents

[0048] ① Instrument

[0049] Ultra-high performance liquid chromatography-quadrupole-time-of-flight mass spectrometry, including Agilent 1290 ultra-high performance liquid chromatography and ABSciex 6600 triple time-of-flight mass spectrometer (AB Sciex, USA), Phenomenex Kinetex C18100A chromatographic column (1.7μm, 2.1×100mm, ...

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Abstract

An application method of a serum lipid biomarker in NSCLC early diagnosis includes the following steps: collecting serum samples of NSCLC patients, patients with benign lung lesions and normal people;pretreating the serum samples, and detecting lipid metabolism markers in each serum sample by utilizing a method of ultra-high performance liquid chromatography-quadrupole-time of flight mass spectrometry; screening NSCLC-related differential lipid metabolism markers through a method of multivariate pattern recognition analysis; screening out key metabolic pathways with highest correlation with the lipid metabolites through KEGG analysis and metabolic pathway analysis of NSCLC differential lipid metabolites; performing network analysis of "gene-enzyme-reaction-metabolite" of the NSCLC differential lipid metabolites, to obtain a network graph of the NSCLC differential lipid metabolites; and screening and obtaining the serum lipid biomarker for the NSCLC early diagnosis through integrationof the screening of the NSCLC differential lipid metabolism markers and analysis results of the metabolic pathways.

Description

technical field [0001] The invention relates to an application method of serum lipid biomarkers in the early diagnosis of NSCLC. Background technique [0002] Lung cancer is one of the most common malignant tumors in the world, and it has become the first cause of death from malignant tumors in urban population in my country. Non-small cell lung cancer (NSCLC) includes squamous cell carcinoma (squamous cell carcinoma), adenocarcinoma, and large cell carcinoma. Compared with small cell carcinoma, the growth and division of its cancer cells are slower, and the spread and metastasis are relatively late. Non-small cell lung cancer accounts for about 80% of all lung cancers, and about 75% of the patients are in the advanced stage when they are discovered, and the 5-year survival rate is very low. Traditional imaging and sputum exfoliation cytology are still the main means of screening for early lung cancer, but there are certain missed and misdiagnosed cases. Although pathologic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/574G01N30/02G01N30/72
CPCG01N30/02G01N30/72G01N33/574G01N33/57423G01N33/57484G01N2570/00G01N2800/12
Inventor 陈莹蓉戴利成马志红沈雄荣
Owner HUZHOU CENT HOSPITAL
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