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Qualitative method of phenolic compounds in tea seeds

A qualitative method and compound technology, applied in the field of qualitative research of phenolic compounds, to achieve the effect of good peak shape, improved specificity and accuracy, and good response

Active Publication Date: 2017-05-10
INSPECTION & QUARANTINE TECH CENT OF XIAMEN ENTRY EXIT INSPECTION & QUARANTINE BUREAU
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] In order to solve the qualitative problem of tea seed phenols, the object of the present invention is to provide a qualitative method for tea seed phenols that does not require standard products, has high analytical sensitivity and good selectivity

Method used

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  • Qualitative method of phenolic compounds in tea seeds
  • Qualitative method of phenolic compounds in tea seeds
  • Qualitative method of phenolic compounds in tea seeds

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] Embodiment 1: A kind of qualitative method of tea seed phenolic compound

[0058] Materials and Reagents:

[0059] Tea seeds are collected from Anxi tea garden in Quanzhou City, Fujian Province.

[0060] Acetonitrile, methanol, formic acid (all chromatographically pure): American Sigma-Aldrich Company; n-hexane (chromatographically pure): Xilong Chemical Co., Ltd.; experimental water is self-made ultrapure water; Catechin, gallic acid monohydrate, phthalic acid, 4-hydroxyphenylacetic acid, vanillic acid, cinnamic acid, p-coumaric acid, caffeic acid, ferulic acid, sinapic acid, chlorogenic acid, epicatechin, Apigenin, Kaempferol, Luteolin, Quercetin, Myricetin, Naringenin (all analytical grade standards): Aladdin Reagent Company; Catechin, Epigallocatechin, Epigallocatechin Gallatin Ester (all analytical grade standard): Sigma Company, USA; 0.2 μm organic filter membrane: Whatman Company, UK. Instruments and equipment:

[0061] Agilent 1290 series ultra-high performa...

Embodiment 2

[0082] Embodiment 2: Verification test

[0083] In order to verify the qualitative effect of tea seed phenolic compounds provided by the present invention, the retention time and secondary mass spectrum of the preliminarily identified compounds were further compared with the standards. Under the same chromatographic mass spectrometry conditions as in Example 1, the total ion current chromatogram of the mixed standard substance was obtained, and after accurate mass extraction, the accurate mass extraction chromatogram was obtained ( Figure 10 ). Depend on Figure 10 It can be seen that there are 24 phenolic compounds in tea seeds, and the retention time of the identified phenolic compounds in tea seeds is compared with that of the standard, as shown in Table 1, the difference in the retention time of all 24 identified compounds and the corresponding standard is within Within ±0.1min. At the same time, the deviations between the secondary fragment ions actually collected in ...

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Abstract

The invention discloses a qualitative method of phenolic compounds in tea seeds. The method is characterized in that an ultra-high performance liquid chromatography-quadrupole-time of flight mass spectrometer method is adopted for identifying; tea seed powder is pretreated, and is detected through the ultra-high performance liquid chromatography-quadrupole-time of flight mass spectrometer to obtain a total ion chromatogram and extracted feature ion chromatogram; elements and accurate molecular weight of a compound corresponding to the ion are calculated by Q-TOF qualitative software; the unknown compound is initially identified according to element composition and accurate molecular weight, cracking the known compound by collision-induction; and the variety of phenolic compounds in tea seeds can be deducted according to the ion cracking situation. Compared with an existing phenolic compound qualitative method, the method has the advantages of no standard product, high analyzing sensitivity and good selectivity.

Description

technical field [0001] The invention relates to the qualitative research field of phenolic compounds, in particular to a qualitative method for tea seed phenolic compounds. Background technique [0002] Tea tree (Camellia Sinensis O.Ktze) is a plant of the genus Camellia in the family Theaceae, which originated in the Yunnan-Guizhou Plateau in my country. Plant materials such as tea tree leaves and fruits are rich in polyphenols. Phenolic compounds are an important secondary metabolite in plants, and their molecular structure contains several phenolic hydroxyl groups, which are generally divided into phenolic acids, flavonoids, tannins, flavanols and glycosides, etc. . Catechins are characteristic phenolic substances of Camellia plants, which belong to flavanol compounds, and generally exist in flowers, stems, leaves and fruits of plants of this genus. Studies have shown that plant phenolic compounds are natural antioxidants that can remove NO and ROS free radicals in the...

Claims

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Application Information

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IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 赖国银刘芸林立毅徐敦明孙婷王晓琴张志刚吕美玲
Owner INSPECTION & QUARANTINE TECH CENT OF XIAMEN ENTRY EXIT INSPECTION & QUARANTINE BUREAU
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