Polypeptide Pv26-3 with antimicrobial activity

Abstract

The application provides polypeptide Pv26-3 with antimicrobial activity, belonging to the technical field of genetic engineering drugs. In the polypeptide Pv26-3, tryptophan W replaces the 7th, 11th,21st and 25th sites in the amino acid sequence of a parent molecule Pv26, and lysine K replaces amino acids at the 1st, 12th, 20th and 24th sites, thereby enhancing the hydrophobicity and positive charge of the parent molecule Pv26. Since a hydrophobic group plays a key role in the process of inserting antimicrobial peptide into a cell membrane, the improvement of hydrophobicity is beneficial to the antimicrobial effect of the antimicrobial peptide; the antimicrobial peptide is bonded to the membrane by electrostatic interaction, so as to promote the combination of the hydrophobic group into the membrane, and then the membrane structure is destroyed to cause cell death, so that positive charge is the key factor affecting the binding of the antimicrobial peptide and the membrane; by improving hydrophobicity and positive charge, the binding ability of the antimicrobial peptide and the membrane can be enhanced, the antimicrobial activity and antibacterial effect of antimicrobial peptide can be improved, and at the same time, the action concentration of antimicrobial peptide is lowered.

Description

Technical field [0001] The embodiments of the present application relate to the technical field of genetic engineering drugs, in particular to a polypeptide Pv26-3 with antibacterial activity. Background technique [0002] At present, the abuse of antibiotics is serious, leading to an endless stream of pathogenic bacteria with drug-resistant effects. Antimicrobial peptides have become an ideal alternative to antibiotics because of their unique bactericidal mechanism and the advantage of not being prone to drug resistance. Antimicrobial peptides (AMP) are a class of peptides with antibacterial activity. They were first discovered in insects and are considered to be an essential component of the innate immune system. Antimicrobial peptides are small molecular peptides with relatively small molecular weight, generally below 10kDa. It is generally believed that the mechanism of antibacterial action of antimicrobial peptides is to change the permeability of the cell membrane, and ca...

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Problems solved by technology

[0002] At present, the abuse of antibiotics is serious, leading to the emergence of pathogenic bacteria with drug resistance

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