Polysialic acid fermentation medium, production method of polysialic acids and polysialic acid product

A fermentation medium and polysialic acid technology, applied in the field of polysialic acid products, can solve the problems of low fermentation level, high fermentation cost, and limited application of N-acetylneuraminic acid

Active Publication Date: 2018-09-28
HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, the current fermentation level of polysialic acid is low, generally around 5-6g/L, re

Method used

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  • Polysialic acid fermentation medium, production method of polysialic acids and polysialic acid product
  • Polysialic acid fermentation medium, production method of polysialic acids and polysialic acid product
  • Polysialic acid fermentation medium, production method of polysialic acids and polysialic acid product

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] The production method of the polysialic acid that the present embodiment provides comprises the steps:

[0051] 1 strain activation

[0052] The frozen polysialic acid-producing strain (preservation number CCTCC NO: M 2018103) was streak-inoculated onto solid LB medium, and cultured at 37°C.

[0053] The polysialic acid used was submitted to the China Type Culture Collection Center (CCTCC) on March 6, 2018 at Luojia Mountain, Bayi Road, Wuchang District, Wuhan City, Hubei Province for preservation, and the preservation number CCTCC NO: M 2018103; classification Scientific name: Escherichia coli CASOV-8.

[0054] 2 Cultivation of primary seeds:

[0055] After a single colony grows, pick a single colony and inoculate it into a Erlenmeyer flask containing 50 mL of liquid LB seed medium, and culture at 200 rpm at 37°C overnight to obtain a first-grade seed liquid.

[0056] 3 Cultivation of secondary seeds:

[0057] The primary seed solution was transferred to a conical ...

Embodiment 2

[0075] The production method of polysialic acid provided in this example is basically the same as that in Example 1, except that CTAB is not added in this example.

[0076] During the cultivation period, at different time points, the fermentation products were taken to detect their OD 600 and polysialic acid content, the results are as figure 2 shown.

Embodiment 3

[0078] The production method of polysialic acid provided in this example is basically the same as Example 1, the difference is that in this example, B vitamins are not added.

[0079] During the cultivation period, at different time points, the fermentation products were taken to detect their OD 600 and polysialic acid content, the results are as image 3 shown.

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Abstract

The invention discloses a polysialic acid fermentation medium, a production method of polysialic acids and a polysialic acid product, and relates to the technical field of polysialic acid production.The polysialic acid fermentation medium comprises a basal culture medium and additives. The additives include one or combinations of b-vitamins, microelement composites and CTAB (cetyltrimethyl ammonium bromide). The b-vitamins comprise VB1, VB5, VBH and VB12. The microelement composites comprise FeSO4, KIO3, MnC12, CoCl2, CrCl3, ZnSO4, Na2MoO4 and H3BO3. When the polysialic acid fermentation medium is used for fermentation production of the polysialic acids, the content of the polysialic acids can be increased, fermentation cost is reduced, and application of N-acetylneuraminic acid is widened.

Description

technical field [0001] The invention relates to the technical field of polysialic acid production, in particular to a polysialic acid fermentation medium, a polysialic acid production method and polysialic acid products. Background technique [0002] On May 31, 2017, the National Health and Family Planning Commission reviewed and approved the use of 10 new food ingredients such as shea butter, including N-acetylneuraminic acid (Neu5Ac). Neu5Ac is the first contact site for cell information transmission, and its molecular structure is diverse, so Neu5Ac is involved in multiple physiological processes such as cell recognition, signal transduction, tumorigenesis, and fertilization. In addition, Neu5Ac can regulate the anti-inflammatory activity of IgG, enhance infant immunity, affect the integrity, permeability and activity of nerve cells, and promote the development of infant brain. Therefore, the production of N-acetylneuraminic acid has attracted more attention and research...

Claims

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Application Information

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IPC IPC(8): C12P19/26C12R1/19
CPCC12P19/26
Inventor 陈祥松吴金勇孙立洁袁丽霞李翔宇王刚朱薇薇姚建铭费贤春王煜申阳汪娟
Owner HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
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