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Polysialic acid fermentation medium, production method of polysialic acid and polysialic acid product

A fermentation medium and polysialic acid technology, applied in the field of polysialic acid products, can solve the problems of high fermentation cost, low fermentation level, and limited application of N-acetylneuraminic acid, and achieve the effect of reducing fermentation cost and increasing content

Active Publication Date: 2021-06-04
HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, the current fermentation level of polysialic acid is low, generally around 5-6g / L, resulting in higher fermentation costs, which in turn limits the application of N-acetylneuraminic acid

Method used

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  • Polysialic acid fermentation medium, production method of polysialic acid and polysialic acid product
  • Polysialic acid fermentation medium, production method of polysialic acid and polysialic acid product
  • Polysialic acid fermentation medium, production method of polysialic acid and polysialic acid product

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] The production method of the polysialic acid that the present embodiment provides comprises the steps:

[0051] 1 strain activation

[0052] The frozen polysialic acid-producing strain (preservation number CCTCC NO: M 2018103) was streak-inoculated onto solid LB medium, and cultured at 37°C.

[0053] The polysialic acid used was submitted to the China Type Culture Collection Center (CCTCC) on March 6, 2018 at Luojia Mountain, Bayi Road, Wuchang District, Wuhan City, Hubei Province for preservation, and the preservation number CCTCC NO: M 2018103; classification Scientific name: Escherichia coli CASOV-8.

[0054] 2 Cultivation of primary seeds:

[0055] After a single colony grows, pick a single colony and inoculate it into a Erlenmeyer flask containing 50 mL of liquid LB seed medium, and culture at 200 rpm at 37°C overnight to obtain a first-grade seed liquid.

[0056] 3 Cultivation of secondary seeds:

[0057] The primary seed solution was transferred to a conical ...

Embodiment 2

[0075] The production method of polysialic acid provided in this example is basically the same as that in Example 1, except that CTAB is not added in this example.

[0076] During the cultivation period, at different time points, the fermentation products were taken to detect their OD 600 and polysialic acid content, the results are as figure 2 shown.

Embodiment 3

[0078] The production method of polysialic acid provided in this example is basically the same as Example 1, the difference is that in this example, B vitamins are not added.

[0079] During the cultivation period, at different time points, the fermentation products were taken to detect their OD 600 and polysialic acid content, the results are as image 3 shown.

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Abstract

The invention discloses a polysialic acid fermentation medium, a polysialic acid production method and a polysialic acid product, and relates to the technical field of polysialic acid production. The polysialic acid fermentation medium disclosed by the present invention includes a basal medium and additives, and the additives include any one or more combinations of the following components: B vitamins, trace element combinations and CTAB (cetyl trimethyl ammonium bromide), B vitamins including: VB 1 , VB 5 , VBH and VB 12 , the trace element combination includes: FeSO 4 、KIO 3 , MnCl 2 、CoCl 2 , CrCl 3 , ZnSO4, Na 2 MoO 4 and H 3 BO 3 . Using the polysialic acid fermentation medium to ferment and produce polysialic acid can increase the content of polysialic acid, thereby reducing fermentation costs and expanding the application of N-acetylneuraminic acid.

Description

technical field [0001] The invention relates to the technical field of polysialic acid production, in particular to a polysialic acid fermentation medium, a polysialic acid production method and polysialic acid products. Background technique [0002] On May 31, 2017, the National Health and Family Planning Commission reviewed and approved the use of 10 new food ingredients such as shea butter, including N-acetylneuraminic acid (Neu5Ac). Neu5Ac is the first contact site for cell information transmission, and its molecular structure is diverse, so Neu5Ac is involved in multiple physiological processes such as cell recognition, signal transduction, tumorigenesis, and fertilization. In addition, Neu5Ac can regulate the anti-inflammatory activity of IgG, enhance infant immunity, affect the integrity, permeability and activity of nerve cells, and promote the development of infant brain. Therefore, the production of N-acetylneuraminic acid has attracted more attention and research...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/26C12R1/19
CPCC12P19/26
Inventor 陈祥松吴金勇孙立洁袁丽霞李翔宇王刚朱薇薇姚建铭费贤春王煜申阳汪娟
Owner HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI