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InDel primer identifying late-bolting QTL located on radish R02 chromosome and application of primer

A chromosome and indel520 technology, applied in the biological field, can solve the problems of insufficient linkage distance, different, difficult to apply, etc., and achieve the effect of saving the cost and time of plant field management

Active Publication Date: 2018-09-28
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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Problems solved by technology

However, the above research results are still difficult to apply in molecular marker-assisted screening of radish bolting resistance.
On the one hand, it is because the linkage distance between the obtained molecular markers and related genes is not close enough. On the other hand, because plant bolting and flowering is a complex network system, which is affected and regulated by multiple genes, different crops or different materials of the same crop can affect each other. Molecular mechanisms underlying bolting and flowering may differ

Method used

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  • InDel primer identifying late-bolting QTL located on radish R02 chromosome and application of primer
  • InDel primer identifying late-bolting QTL located on radish R02 chromosome and application of primer
  • InDel primer identifying late-bolting QTL located on radish R02 chromosome and application of primer

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] Acquisition of Molecular Markers for Late Bolting Trait in Radish

[0061] The invention utilizes a conventional map-position cloning method to identify and clone genes related to late bolting traits of radish.

[0062] 1. Genetic segregation population construction and phenotypic identification

[0063] Using the Japanese late bolting material "Ninengo" and the Chinese early bolting material "Maer" as parents, a F 2 group. Parents, F in the spring of 2016 1 , F 2 The bolting and flowering traits of individual plants in the population were investigated. The survey method is: survey the budding and flowering of individual plants every other day. Budding time is the number of days required from planting to visible flower buds; flowering time is the number of days required from planting to the opening of the first flower. The budding time and flowering time of the late bolting material "Ninengo" were 91 days and 110 days, respectively, and the budding time and flower...

Embodiment 2

[0073] Application of InDel520 and InDel535 in Identification of Radish Late Bolting and Early Bolting Materials

[0074] 1. Identification of late-bolting and early-bolting radish materials by molecular markers

[0075] 1) DNA extraction

[0076] Genomic DNA of 183 kinds of radish materials to be tested in Table 2 were extracted by conventional CTAB method. The 183 tested radish materials in Table 2 are the F1 generation obtained by crossing the parents Ninengo and Maer, and the F2 individual plants obtained by selfing the F1.

[0077] 2) PCR amplification and detection

[0078] The above-mentioned InDel520 primer pair and InDel535 primer pair were used for PCR amplification of the radish material to be tested.

[0079] PCR reaction system: containing 100ng genomic DNA, 1μl 10×PCR Buffer, 0.8μl dNTPs, 0.2μl (10μM) of the above-mentioned upstream and downstream primers, 1U Taq enzyme, plus ddH 2 0 to 10 μl. The PCR amplification program was: pre-denaturation at 94°C for 4...

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Abstract

The invention provides an InDel primer identifying late-bolting QTL located on a radish R02 chromosome and application of the prime, and belongs to the technical field of biotechnologies. The InDel primer comprises an InDel520 primer pair and an InDel535 primer pair. The InDel primer can identify radish late-bolting QTL, and the accuracy for performing molecular marker assisted identification on radish late-bolting materials is 100%.

Description

technical field [0001] The invention belongs to the technical field of biotechnology, and in particular relates to an InDel primer for identifying a late bolting QTL located on the R02 chromosome of radish and an application thereof. Background technique [0002] Radish originated in my country and is an important vegetable crop in my country. It has long been the "home vegetable" of urban and rural residents in my country. The annual sowing area is 16 million mu, accounting for 6% of the entire vegetable production in my country. Radish is a long-day plant. After low-temperature vernalization, it turns from vegetative growth to reproductive growth under long-day conditions, thereby bolting and flowering. The most important impact of immature bolting and flowering on radish production is the transition from vegetative growth to reproductive growth, which inhibits the expansion of fleshy roots, resulting in a decrease in yield and quality. Breeding radish varieties with lat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 张丽王庆彪
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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