Unlock instant, AI-driven research and patent intelligence for your innovation.

Maize recessive male sterility mutant gene ms30 The function flags and their application

A technology of ms30-in-1f and ms30-in-2r is applied in the field of functional marker of maize recessive nuclear male sterility gene ms30, which can solve the problem of incomplete linkage between marker traits and sterility, and has not been popularized and applied. Difficulty in character identification

Active Publication Date: 2020-10-13
UNIV OF SCI & TECH BEIJING +1
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, since the discovery of maize recessive genetic sterile materials, people have used traditional breeding techniques to explore and study various marker traits of the genetic sterile gene, such as using the close linkage relationship between marker traits and sterility, The grain color marker system method, the yellow-green seedling linkage marker method, and the multi-filament linkage marker system have been developed. However, due to the incomplete linkage between marker traits and sterility, difficulties in the identification of marker traits, and delays in the identification period, these methods and attempts have been in the field. It has not been widely used in corn production

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Maize recessive male sterility mutant gene <i>ms30</i> The function flags and their application
  • Maize recessive male sterility mutant gene <i>ms30</i> The function flags and their application
  • Maize recessive male sterility mutant gene <i>ms30</i> The function flags and their application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1: Obtaining maize male sterile mutant material

[0037] The test material of the present invention is the normal fertile inbred line of maize Ms30 Sterile plants obtained by genetic mutations show complete non-pollen male sterility, such as figure 1 As shown, the specific manifestations are: the anthers become smaller, whitish, and the pollen cannot be stained by iodine-potassium iodide. The CMS material is named ms30-6028 .

Embodiment 2

[0038] Example 2: ms30 Mutation site analysis and development of functional markers

[0039] male sterile material ms30-6028 yes Ms30 caused by point mutations. Such as figure 2 As shown, the mutant gene ms30 There is a 464bp sequence deletion at the +1214 position.

[0040] According to mutant ms30-6028 mutant gene ms30 The missing sequence and its position, using primer design software, can develop a series of wild-type Ms30 and mutant ms30 Specific functional co-segregation molecular markers of genes. For example, primer sequences designed in whole or in part in the deletion sequence can be used as wild-type Ms30 Gene-specific markers. Primer sequences were designed on both sides of the deletion sequence, from wild-type Ms30 Genes and Mutants ms30 The size of the amplified bands in the gene is different, and the wild type can be detected at the same time Ms30 Genes and Mutants ms30 Gene.

Embodiment 3

[0041] Example 3: Development of functional markers ms30-IN1, ms30-IN2, ms30-IN3, ms30-IN4 and ms30-IN5

[0042] In the present invention, for ms30 For the mutation site, primers were designed using Primer5.0 software, and five pairs of functional molecular markers were developed: ms30-IN1, ms30-IN2, ms30-IN3, ms30-IN4, and ms30-IN5, among which ms30-IN1, ms30-IN2 , ms30-IN3 and ms30-IN4 can specifically detect maize mutants ms30-6028 and the mutant gene in the maize sterile material transduced by it ms30 , and can simultaneously distinguish wild-type Ms30 Genes and Mutants ms30 Gene. And ms30-IN5 is wild type Ms30 Gene-specific markers. The position diagram of the amplification primers for the developed five pairs of functional markers ms30-IN1, ms30-IN2, ms30-IN3, ms30-IN4 and ms30-IN5 is as follows image 3 As shown in A, the amplification primers of each marker are in Ms30 with ms30 The corresponding specific positions in the gene alignment sequence are as fol...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to functional markers for a maize recessive nuclear male sterility mutant gene ms30, a functional marker development method and application of the functional markers. According to mutant sites of the maize recessive nuclear male sterility mutant gene ms30, including but not limited to five functional markers of ms30-IN1, ms30-IN2, ms30-IN3, ms30-IN4, ms30-IN5, ms30-IN5 and the like are designed, wherein the four functional markers of ms30-IN1, ms30-IN2, ms30-IN3 and ms30-IN4 can specifically detect a maize mutant ms30-6028 and the mutant gene ms30 in a maize sterility material transformed by the maize mutant ms30-6028, and can simultaneously distinguish a wild-type Ms30 gene and a mutant-type ms30 gene, and the functional marker ms30-IN5 is a specific dominant markerfor the wild-type Ms30 gene. The designed functional markers can accurately detect and mark allele genotypes of the maize nuclear male sterility gene ms30 or a fertility gene Ms30, can be used for identifying male sterility strains and sifting target single plants, have important application value in cultivating of maize male sterility lines, sterile hybrid seed production and molecular marker-assisted selection, and are significant in improving the efficiency of maize crossbreeding and seed production, guaranteeing the seed production quality and the like.

Description

technical field [0001] Generally, the present invention is in the fields of crop molecular breeding, molecular biology and genetic engineering. Concretely relates to a kind of maize recessive male sterility gene ms30 Functional flags, functional flag development methods and applications. Background technique [0002] Plant male sterility (male sterility, MS) refers to the abnormal development of male organs in higher plants, unable to produce functional male gametes (pollen), but the normal development of female organs, which can receive normal male gametes and fertilize and set fruit, and can The phenomenon in which sterility is passed on to offspring. [0003] Maize is an important food crop, and its male sterile materials are of great value in the study of maize male flower development mechanism, genetic breeding application, heterosis research and application. Maize male sterility can be divided into three types according to the differences in sterility inheritance mo...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12N15/11A01H1/04A01H1/02
CPCA01H1/02A01H1/04C12Q1/6895C12Q2600/13C12Q2600/156
Inventor 万向元安学丽谢科吴锁伟董振营田有辉李金萍刘欣洁张思淼朱涛涛鲍建喜
Owner UNIV OF SCI & TECH BEIJING