Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Molecular clone extracting and verifying method

A technology of molecular cloning and substrate, applied in the direction of biochemical equipment and methods, material inspection products, microbial measurement/inspection, etc., can solve the problems of limiting practical application, and achieve the effect of minimum extraction cost and low cost

Pending Publication Date: 2018-09-28
塞勒密斯株式会社 +1
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these methods can only analyze short sequences, which limits their practical application

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Molecular clone extracting and verifying method
  • Molecular clone extracting and verifying method
  • Molecular clone extracting and verifying method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0020] The invention will be described in detail with reference to various embodiments. General methods for extracting and characterizing molecular clones include 1) obtaining molecular clones from microorganisms such as E. Amount of DNA, and 4) Analysis of the amplified DNA by Sanger sequencing. In contrast, the method of the present disclosure enables the rapid acquisition of information on the sequence of molecular clones and Retrieve molecular clones. These advantages are attributed to the non-contact extraction of molecular clones and the characteristics of next-generation sequencing. The present disclosure utilizes the characterized methods of molecular cloning to enable gene synthesis at least 10-fold less expensive than conventional methods.

[0021] Embodiments of the present invention will now be described in detail with reference to the accompanying drawings. Rather, these embodiments are provided so that this disclosure will be thorough and complete, and will f...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
diameteraaaaaaaaaa
wavelengthaaaaaaaaaa
Login to View More

Abstract

Provided is a molecular clone extracting and verifying method which comprises the steps of: providing a substrate on which molecular clones are formed; applying energy in a contactless manner to desired molecular clones from among the molecular clones and extracting the desired molecular clones from the substrate; chemically linking DNA barcodes and base sequences of the extracted molecular clones; and analyzing the base sequences, to which the DNA barcodes have been linked, through a parallel sequencing analysis technique.

Description

technical field [0001] The present disclosure relates to methods of extracting and characterizing molecular clones. Background technique [0002] Antibodies are glycoproteins produced by antigens (eg, viruses and bacteria) that stimulate an immune response in vivo, which specifically bind the antigen to induce extracellular stimulation. Antibodies bind to specific proteins with high selectivity. For this reason, antibodies have high utility in the fields of biology, biotechnology, and medicine, ranging from basic research related to labeling / observation of target proteins to medical applications related to the regulation of abnormal protein activity in patients. In particular, the use of antibodies as next-generation new drug platforms such as antibody drug conjugates (ADCs) and chimeric antigen receptor-T cell (CART) therapeutics has been increasing in recent new drug development. [0003] In general, the usefulness of antibodies depends on their ability and specificity t...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/10C12Q1/6869G01N33/68C40B40/08C40B40/06
CPCC40B40/08G01N33/68C12Q1/6848C12Q1/6806C12Q2523/303C12Q2523/308C12Q2535/122C12Q2563/159C12Q2563/179C12N15/1037C12N15/1093C12Q1/6837C12Q1/6869C40B40/06C12Q2563/185C12Q2565/50C12Q2565/619C12Q2537/143C12N15/10C12N15/70C12Q1/6874C12Q1/6876
Inventor 柳兑勋金孝基韩孝俊权圣勋
Owner 塞勒密斯株式会社
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com