Primary tumor cell culture medium, culture method and application

Abstract

The invention belongs to the technical field of medicine, and specifically relates to a primary tumor cell culture medium, culture method and application. The primary tumor cell culture method provided by the invention comprises the steps as follows: preparing a primary cell culture medium which comprises the following components: hydrocortisone, EGF, Insulin, a ROCK inhibitor, but not contains cholera toxin, and is an improvement of the existing primary cell culture medium; culturing tumor tissue epithelial cells on laid-out trophoblast cells by using the primary cell culture medium, and enabling the tumor tissue epithelial cells to proliferate rapidly under the combined action of growth factors secreted by the trophoblast cells and nutrient factors contained in the culture medium; and digesting and sub-culturing the tumor tissue epithelial cells when the tumor tissue epithelial cells grow to a cell density of about 80% to 90%. A convenient primary cell culture method is used to obtain immortalized cells possessing the biological characteristics of a patient's own tumor, and the problem of immortalization of the primary culture of tumor cells is solved, thereby realizing personalized treatment for the patient.

Description

technical field [0001] The invention belongs to the technical field of medicine, and specifically relates to a method for primary tumor epithelial cell culture and drug evaluation, including a primary tumor epithelial cell culture medium, a culture method, and a drug evaluation method. Background technique [0002] In recent years, with the gradual development of screening technology, surgical technology and the application of standardized radiotherapy and chemotherapy technology, the clinical prevention and treatment of cancer have made great progress, but the five-year survival rate of many advanced cancers has not be significantly improved. The main reason is that the curative effect of clinical chemotherapy drugs is very limited. Due to the degree of tumor progression, gene mutation, biological characteristics, drug resistance and heterogeneity of different cancer patients are not completely the same, so their sensitivity to clinical chemotherapy drugs Different, the re...

AI Technical Summary

Problems solved by technology

However, the modeling time of PDX model is long, the cost is too high, there is a lack of immune microenvironment, and the success rate is nearly 25%, so it is difficult to be widely used in clinical practice to benefit patients

[0004] Tumor primary cell culture has always been an important method for clinical evaluation of drugs, but due to the difficulty and success rate of different tumor tissues, it has been difficult to form a stable method

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