Primary tumor cell culture medium, culture method and application

Abstract

The invention belongs to the technical field of medicine, and specifically relates to a primary tumor cell culture medium, a culture method and an application thereof. The method for cultivating primary tumor cells of the present invention comprises: preparing a primary cell culture medium, the components of which include: hydrocortisone, EGF, Insulin, and ROCK inhibitors, but do not contain cholera toxin, and are used for culturing existing primary cells Improvement of the base; use the above-mentioned primary cell culture medium to culture tumor tissue epithelial cells on the paved trophoblast cells, and the tumor tissue epithelial cells rapidly grow under the joint action of the growth factors secreted by the trophoblast cells and the nutritional factors contained in the medium. Proliferate, digest and passage when the epithelial cells of the tumor tissue grow to about 80%~90% of the cell density. The invention utilizes a convenient primary cell culture method to obtain immortalized cells with the biological characteristics of the patient's own tumor, which solves the problem of primary culture immortalization of tumor cells, thereby realizing personalized treatment for patients.

Description

technical field [0001] The invention belongs to the technical field of medicine, and specifically relates to a method for primary tumor epithelial cell culture and drug evaluation, including a primary tumor epithelial cell culture medium, a culture method, and a drug evaluation method. Background technique [0002] In recent years, with the gradual development of screening technology, surgical technology and the application of standardized radiotherapy and chemotherapy technology, the clinical prevention and treatment of cancer have made great progress, but the five-year survival rate of many advanced cancers has not be significantly improved. The main reason is that the curative effect of clinical chemotherapy drugs is very limited. Due to the degree of tumor progression, gene mutation, biological characteristics, drug resistance and heterogeneity of different cancer patients are not completely the same, so their sensitivity to clinical chemotherapy drugs Different, the re...

AI Technical Summary

Problems solved by technology

However, the modeling time of PDX model is long, the cost is too high, there is a lack of immune microenvironment, and the success rate is nearly 25%, so it is difficult to be widely used in clinical practice to benefit patients

[0004] Tumor primary cell culture has always been an important method for clinical evaluation of drugs, but due to the difficulty and success rate of different tumor tissues, it has been difficult to form a stable method

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