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Rapid PCR detection method for pseudoalteromonas nigrifaciens

A technology of pseudoalteromonas and detection method, applied in the field of rapid PCR detection of pseudoalteromonas, can solve the problems of difficult detection, serious disease, poor detection accuracy, etc., and achieve the effect of high detection sensitivity

Active Publication Date: 2018-10-09
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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Problems solved by technology

[0005] In order to solve the existing problems of serious disease of Porphyra zebra green spot disease, difficult detection and poor detection accuracy, we proposed a rapid PCR detection method for Pseudoalteromonas

Method used

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  • Rapid PCR detection method for pseudoalteromonas nigrifaciens
  • Rapid PCR detection method for pseudoalteromonas nigrifaciens
  • Rapid PCR detection method for pseudoalteromonas nigrifaciens

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Embodiment 1

[0040] Strain and PCR specificity

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Abstract

The invention provides a rapid PCR detection method for pseudoalteromonas nigrifaciens. The rapid PCR detection method uses a pseudoalteromonas nigrifaciens gene as a detection target gene, and comprises the detection steps of primer synthesis, DNA extraction, PCR reaction, electrophoresis detection and result determination. The rapid PCR detection method is the first method for detecting the pathogen of agar green spot disease in China, and the detection accuracy is high. Porphyra yezoensis is artificially infected with the pseudoalteromonas nigrifaciens, three pairs of specific primers are used for detecting the infected agar respectively, and the positive detection rate is 100%. The detection sensitivity is high, and the three pairs of primers can be used for specifically detecting thepseudoalteromonas nigrifaciens, and the detection sensitivity is 37 CFU, 3.7 CFU and 37 CFU of bacteria and 2.37pg, 23.7fg and 2.37 pg of DNA, respectively.

Description

technical field [0001] The invention relates to the field of microbiology and disease of aquaculture animals, in particular to a rapid PCR detection method for Pseudoalteromonas causing Porphyra variegata green spot disease. Background technique [0002] Porphyra is an important economic algae in the world, and there are about 134 species in the world. It tastes delicious and is rich in vitamins, minerals and antioxidants, while it is low in energy and rich in dietary fiber. Can improve the cecal environment and increase beneficial microflora, lower blood pressure and lower blood lipids, and anti-tumor activity [7] , Improve the activity of macrophages, and have potential pharmaceutical, medicinal and research values. Therefore, seaweed has the reputation of "treasure house of nutrition" again, and is deeply loved by urban and rural people. With the development of Porphyra zebra cultivation industry, its disease problem has become more and more prominent, which has become...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/689C12Q1/686C12Q1/04
CPCC12Q1/686C12Q1/689C12Q2565/125
Inventor 李杰莫照兰阎永伟李贵阳杨慧超
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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