Method for preparing duck tembusu and avian influenza bivalent inactivated vaccines and products obtained through method

Abstract

The invention discloses a method for preparing duck tembusu and avian influenza bivalent inactivated vaccines and products obtained through the method. The method comprises the following steps: respectively inoculating duck tembusu and avian influenza viral strains by adopting EB66 passage cells; respectively harvesting the duck tembusu and avian influenza viruses when the titer of the virus ELD50or EID50 is the highest, and storing the duck tembusu and avian influenza viruses; respectively inactivating the duck tembusu and avian influenza viruses; and completely mixing the inactivated duck tembusu and avian influenza viruses in tween-80, thus obtaining a virus mixed solution, adding a sterilized oil phase adjuvant into the virus mixed solution, and carrying out mixing emulsification, thus preparing the duck tembusu and avian influenza bivalent inactivated vaccines. According to the method, the culture of the duck tembusu viruses and avian influenza viruses is carried out respectivelyby adopting the EB66 passage cells, and the prepared duck tembusu and avian influenza bivalent vaccines have the advantages that the preparation technology is stable, the batch-to-batch differences are small, the quality is controllable, the yield and quality are obviously improved, etc.

Description

technical field [0001] The invention relates to the technical field of veterinary biological products, in particular to a method for producing duck tampusu and avian influenza dual inactivated vaccine and its product. Background technique [0002] Duck Tembusu virus disease (Duck Tembusu Virus Strain), also known as laying duck yellow virus disease, is a disease caused by Duck Tembusu virus of the family Flaviviridae. Ovarian oophoritis is an acute infectious disease characterized by major lesions. Since the disease was discovered in my country in 2010, it has spread widely, and it occurs every year in the duck breeding areas of various provinces. The disease has developed from the initial peak egg production period in summer to the present irregular occurrence, showing an irregular change, which is extremely harmful to the laying duck breeding industry and causes serious economic losses. [0003] Avian Influenza (H9 subtype) (AvainInfluenzaH9subtype) is an infection and / o...

AI Technical Summary

Problems solved by technology

At present, the development of duck Tembusu and avian influenza vaccine mainly adopts traditional chicken embryo, duck embryo, and roller bottle cultured cells as materials for virus propagation and antigen preparation. Although this method has mature technology and low technical requirements, it is labor-intensive. Large, low production efficiency, and there is a risk of foreign aid virus contamination due to the use of chicken embryos and duck embryos to make seedlings. The difference between vaccine batches is large and the quality is unstable. It is different from the current method of preparing vaccines using pure suspension large-scale culture of animal cells Therefore, we are looking for a passage cell line with high abundance of avian influenza and Tambusu virus receptors, no tumorigenicity, no exogenous virus contamination, and large-scale serum-free pure suspension culture for the preparation of safe and efficient combined vaccines. It has become a trend, and it is particularly urgent to use the same process and culture carrier (cell) to cultivate two or more vaccine viruses