DNA extracting method for fetal chromosome aneuploid detection

Abstract

The invention discloses a DNA extracting method for fetal chromosome aneuploid detection. The method comprises the steps of 1, processing a buffer solution and a reagent; 2, adding 20 UL Proteinase Kand 30 UL Magpure Particles G into a plasma tube, and performing oscillation and uniform mixing; adding 500 UL Buffer MLF, and performing vortex uniform mixing; transferring the plasma tube into a constant-temperature uniform mixer preheated to 37 DEG C, and regulating the rotating speed to 1,000 rpm; placing the mixer on a magnetic frame for 3 min, abandoning the solution after clarification is performed, and taking down a centrifugal tube; adding 500 UL Buffer MW I, performing oscillation and uniform mixing, performing instant centrifuging, placing the mixture on the magnetic frame for 3 min, and sucking out liquid inside the tube after adsorption is completed; 3, adding 500 UL Buffer MW II, performing oscillation and uniform mixing, performing instant centrifuging, placing the mixture on the magnetic frame, and sucking out liquid inside the tube after adsorption is completed; 4, adding Buffer AE preheated to 37 DEG C, re-suspending magnetic beads, and performing standing; 5, performing high-speed micro-separation, placing the mixture on the magnetic frame, sucking the DNA solution into a correspondingly-numbered 1.5 ml novel tubes after clarification is performed, and obtainingthe extracted DNA solution; the DNA extracting yield is higher, and storage and quality control are more reasonable and clearer.

Description

technical field [0001] The invention belongs to the field of non-invasive prenatal screening and detection, in particular to a method for extracting DNA in fetal chromosomal aneuploidy detection. Background technique [0002] The rapid acquisition of genetic information of living organisms is of great significance to the research of life sciences. The reliance of first-generation sequencers on electrophoretic separation technology makes it difficult to further improve the speed and degree of parallelization of analysis, and it is also difficult to reduce sequencing costs through miniaturization. After continuous technological development and improvement, at the beginning of the 21st century, the second-generation sequencing technology marked by Roche454 technology, Illumina's GenomeAnalyzer technology and ABI's Solid technology was born. The second generation technology greatly reduces the cost of sequencing, and also greatly improves the sequencing speed and maintains high...

AI Technical Summary

Problems solved by technology

To solve a DNA extraction method in the detection of fetal chromosomal aneuploidy, the cost is high, the process is unscientific, the standard is not clear, the practical operation effect is not good, the operation is difficult, it is difficult to copy the operation, the DNA extraction process is not standard, the preservation and quality Technical problems affecting subsequent testing and the success rate of subsequent links