Surface immobilized yeast fermentation technology

A technology of immobilized yeast and technology, which is applied in the field of surface immobilized yeast fermentation, can solve problems such as poor effect, and achieve the effect of low cost, high substrate conversion rate and good adsorption effect

Inactive Publication Date: 2018-10-16
NANJING HIGH TECH UNIV BIOLOGICAL TECH RES INST CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention is to provide a surface-immobilized yeast fermentation technology to solve the problems of poor effect in the prior art

Method used

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  • Surface immobilized yeast fermentation technology
  • Surface immobilized yeast fermentation technology
  • Surface immobilized yeast fermentation technology

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Example 1 Preparation of fuel ethanol by using synthetic medium as fermentation raw material and adopting bamboo fiber immobilized yeast

[0044] First, put the bamboo fiber (thickness 0.15cm) cut into 15cm in diameter into polytetrafluoroethylene (PTFE) made into a 20cm in diameter spherical grid as shown in Figure 1, and prepare an immobilized carrier (Figure 2); then Place the immobilization medium at 150cm 2 The filling amount per L is placed in a 15L reactor with a height-to-diameter ratio of 2:1. Then the cultured strains were added to the fermenter, and fixed at a flow rate of 30 L / h for 36 hours. Until the bacterial cell adsorption concentration is 200 million / ml. Then drain to waste. Then feed the fermentation medium (glucose concentration is 220g / L), the liquid content is 75%, and carry out circular fermentation, wherein the temperature is 33°C, the pH of the fermentation broth is 4.5, and the flow rate is 30L / h. The average fermentation period of 5 batche...

Embodiment 2

[0045] Example 2 Production of fuel ethanol using cassava liquefied mash medium as fermentation raw material and cotton fiber immobilized yeast

[0046] First, put the cotton fiber cut into a diameter of 20cm into a spherical grid with a diameter of 25cm made of polyethylene to prepare an immobilization medium; 2 The filling amount per L is placed in a 15L reactor with an aspect ratio of 1.8:1. Then the cultured strains were added to the fermenter, and fixed at a flow rate of 15 L / h for 48 hours. Until the bacterial cell adsorption concentration is 250 million / ml. Then prepare cassava liquefied mash: the ratio of solid to liquid is 1:2.5, add liquefaction enzyme (15U / g cassava dry weight) to liquefy at 85°C for 2 hours, and discharge the immobilized seed waste liquid after cooling. Then add cassava liquefied mash, and add glucoamylase (180U / g cassava dry weight), the liquid content is 75%, and carry out circular fermentation, wherein the temperature is 35 ° C, the pH of the ...

Embodiment 3

[0047] Example 3 Production of fuel ethanol using high-concentration cassava liquefied mash medium as fermentation raw material and immobilized yeast with cotton fiber

[0048] Firstly, the cotton fiber cut into a diameter of 8 cm was put into a spherical grid with a diameter of 12 cm made of polyvinyl fluoride to prepare an immobilization medium; 2The filling amount per L is placed in a 50L reactor with an aspect ratio of 1.8:1. Then the cultured strains were added to the fermenter, and fixed at a flow rate of 30 L / h for 48 hours. Until the bacterial cell adsorption concentration is 200 million / ml. Then prepare cassava liquefied mash: the ratio of solid to liquid is 1:2, add liquefying enzyme (18U / g cassava dry weight) to liquefy at 90°C for 1.5 hours, and discharge the immobilized seed waste liquid after cooling. Then add cassava liquefied mash, and add glucoamylase (200U / g cassava dry weight), the liquid content is 75%, and carry out circular fermentation, wherein the tem...

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Abstract

The invention discloses a surface immobilized yeast fermentation technology. According to the surface immobilized yeast fermentation technology, a fiber material is immobilized into a spherical grid,and then yeast is immobilized to a fiber carrier through an adsorption effect. According to the surface immobilized yeast fermentation technology, the defects in the prior art are overcome, the immobilized method is low in input cost, convenient to operate, stable in technique and good in adsorption effect, and has the great protecting and promoting effects on cell activity and growth. Yeast cellswith immobilized media is adopted to ferment to produce ethyl alcohol, it can be effectively ensured that the yeast is efficiently and stably fermented for a long term, and the conversion rate of a substrate can further be increased.

Description

technical field [0001] The invention belongs to the technical field of industrial biology, and in particular relates to a surface-immobilized yeast fermentation technology. Background technique [0002] With the shortage of natural resources and the increasingly prominent environmental problems, the status of biological fermentation technology is becoming more and more important, and it has also achieved rapid development in recent years. However, traditional liquid submerged fermentation still has natural defects, such as poor stability of cell growth due to mechanical damage and unsuitable liquid environment, prone to significant cell death and autolysis, and low space-time efficiency, resulting in the development of biological fermentation technology. Maturity is difficult to compare with chemical catalysts, which is also one of the main bottlenecks for biological manufacturing to replace chemical manufacturing. [0003] In view of the deficiencies in the traditional fer...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/12C12P7/06
CPCC12N11/12C12P7/06Y02E50/10
Inventor 陈勇刘庆国应汉杰邹亚男赵南刘桂文
Owner NANJING HIGH TECH UNIV BIOLOGICAL TECH RES INST CO LTD
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