Application and preparation method of a kind of compound malformin C
A compound and extraction technology, applied in the biological field, can solve problems such as insufficient biological control methods for red tide
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
preparation example Construction
[0019] The present invention also provides a preparation method of compound Malformin C, the method comprising:
[0020] Step 1. Inoculate Aspergillus sp.SCSIOW2 into the liquid culture medium, culture on a shaker at a temperature of 28-32°C and a rotation speed of 180-250r / min for 3-10 days to obtain a fermentation broth;
[0021] Aspergillus sp.SCSIOW2 has been preserved in the China Center for Type Culture Collection (CCTCC for short) on [October 21, 2015], and its address is Bayi Road, Wuchang District, Wuhan City, Hubei Province Mount Luojia, the preservation number is CCTCC NO:M2015628, named Aspergillussp.SCSIOW2).
[0022] Step 2, using ethyl acetate to extract and separate the fermentation broth to obtain the compound Malformin C.
[0023] Specifically, the formula of the liquid medium is: 3% sea salt, 2% glucose, 1% peptone and 0.5% yeast extract by mass percentage.
[0024] Specifically, the extraction methods include:
[0025] Add ethyl acetate to the fermentati...
Embodiment
[0032] 1. Bacteria culture
[0033] 1) Prepare LB solid medium, add 2% agar, 1% peptone, 0.5% NaCl, and 0.5% yeast extract to deionized water, stir to dissolve, adjust the pH to about 7.5, and store at 121°C Autoclave for 20min.
[0034] 2) After the culture medium is cooled to about 50°C for strain fermentation, pour it onto a plate. After the plate is condensed, burn the inoculation loop with fire, pick the Aspergillus hyphae CCTCC NO:M2015628 after cooling, and scratch the plate, and place it in a water-proof constant temperature incubator for 2-3 days at 28°C, and store it in a refrigerator at 4°C .
[0035] 3) Prepare liquid medium: add 3% sea salt, 2% glucose, 1% peptone, and 0.5% yeast extract to deionized water, stir and dissolve, adjust the pH to about 7.5, and pack in Erlenmeyer flasks were divided into 38 1L Erlenmeyer flasks of about 250mL each, and autoclaved at 121°C for 20 minutes.
[0036] 4) Inoculation Wait until the liquid medium is cooled to about 30°C,...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com