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Application and preparation method of a kind of compound malformin C

A compound and extraction technology, applied in the biological field, can solve problems such as insufficient biological control methods for red tide

Active Publication Date: 2021-09-10
SHENZHEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The invention provides the application and preparation method of a compound Malformin C, aiming to solve the problem of insufficient methods for biological control of red tide

Method used

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  • Application and preparation method of a kind of compound malformin C
  • Application and preparation method of a kind of compound malformin C
  • Application and preparation method of a kind of compound malformin C

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preparation example Construction

[0019] The present invention also provides a preparation method of compound Malformin C, the method comprising:

[0020] Step 1. Inoculate Aspergillus sp.SCSIOW2 into the liquid culture medium, culture on a shaker at a temperature of 28-32°C and a rotation speed of 180-250r / min for 3-10 days to obtain a fermentation broth;

[0021] Aspergillus sp.SCSIOW2 has been preserved in the China Center for Type Culture Collection (CCTCC for short) on [October 21, 2015], and its address is Bayi Road, Wuchang District, Wuhan City, Hubei Province Mount Luojia, the preservation number is CCTCC NO:M2015628, named Aspergillussp.SCSIOW2).

[0022] Step 2, using ethyl acetate to extract and separate the fermentation broth to obtain the compound Malformin C.

[0023] Specifically, the formula of the liquid medium is: 3% sea salt, 2% glucose, 1% peptone and 0.5% yeast extract by mass percentage.

[0024] Specifically, the extraction methods include:

[0025] Add ethyl acetate to the fermentati...

Embodiment

[0032] 1. Bacteria culture

[0033] 1) Prepare LB solid medium, add 2% agar, 1% peptone, 0.5% NaCl, and 0.5% yeast extract to deionized water, stir to dissolve, adjust the pH to about 7.5, and store at 121°C Autoclave for 20min.

[0034] 2) After the culture medium is cooled to about 50°C for strain fermentation, pour it onto a plate. After the plate is condensed, burn the inoculation loop with fire, pick the Aspergillus hyphae CCTCC NO:M2015628 after cooling, and scratch the plate, and place it in a water-proof constant temperature incubator for 2-3 days at 28°C, and store it in a refrigerator at 4°C .

[0035] 3) Prepare liquid medium: add 3% sea salt, 2% glucose, 1% peptone, and 0.5% yeast extract to deionized water, stir and dissolve, adjust the pH to about 7.5, and pack in Erlenmeyer flasks were divided into 38 1L Erlenmeyer flasks of about 250mL each, and autoclaved at 121°C for 20 minutes.

[0036] 4) Inoculation Wait until the liquid medium is cooled to about 30°C,...

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Abstract

The invention provides the application and preparation method of compound Malformin C, and relates to the field of biotechnology. Wherein, the compound Malformin C is applied to inhibit or kill algae. The compound Malformin C can inhibit the growth of algae in the red tide, stop the growth of algae cells, and even kill the algae cells, so as to control a large number of red tide outbreaks.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to the application and preparation method of a compound Malformin C. Background technique [0002] Red tide (Red tide) refers to a harmful ecological phenomenon that some algae, protozoa or bacteria explosively proliferate or highly gather in the ocean under certain climatic conditions, causing the water body to change color. The harm caused by red tides to marine life and humans can be divided into the following three aspects: first, red tide algae excessively consume dissolved oxygen in seawater, or directly block the respiratory organs of organisms and cause a large number of marine fish deaths; second, some Red tide algae that produce toxins directly lead to the death of marine organisms, and some toxins can cause food poisoning and even death in humans through the upward transmission of some toxins; third, the explosive proliferation of red tide algae causes the imbalan...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K5/12C12P21/04A01N43/90A01P13/00C12R1/66
CPCA01N43/90C07K5/12C12P21/02
Inventor 李晓帆王立岩王珏
Owner SHENZHEN UNIV
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