Determination method for dialysis bag fitting extracellular polymeric substance (EPS) adsorption substrate of anaerobic ammonia oxidizing bacteria
A technology of anammox bacteria and extracellular polymer, which is applied in measurement devices, analytical materials, instruments, etc., to achieve the effect of low experimental cost and simple operation
Inactive Publication Date: 2018-10-19
CHINA UNIV OF MINING & TECH
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[0003] The purpose of the present invention is to provide a dialysis bag fitting method for the determination of the extracellular polymer adsorption substrate of anammox bacteria, to solve the problem of using chemical methods to detect the amount of ammonia nitrogen or nitrite nitrogen adsorbed by EPS
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Embodiment 1
[0090] Example 1: At present, the methods for extracting EPS from anammox bacteria mainly include: EDTA method, ultrasonic method, ion exchange method, centrifugation method, heating method, stirring method, and mixed utilization of two or more methods. Generally speaking, the EDTA method, ultrasonic method, stirring method, and heating method are relatively simple to operate. After treatment, the supernatant containing EPS is generally obtained by centrifugation. The ion exchange method requires the addition of cation exchange resin. The contents of various substances in EPS extracted by various methods are different, and each has its own advantages and disadvantages. The method adopted in this patent is ultrasonic extraction.
[0091] After obtaining the sludge, a certain amount of sludge was placed in a centrifuge tube, centrifuged at 3000rpm / min for 10min, and the supernatant was discarded. Add phosphate buffer solution submerged in the volume of sludge, place at 30°C for...
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The invention discloses a determination method for dialysis bag fitting an extracellular polymeric substance adsorption substrate of anaerobic ammonia oxidizing bacteria, and belongs to determinationmethod of EPS adsorption substrates. The method comprises the following steps: adding an ammonia nitrogen solution or a nitrite nitrogen solution into a pretreated dialysis bag, adding the ammonia nitrogen solution or the nitrite nitrogen solution of the same concentration and an EPS solution have the same concentration and volume in a pure substrate solution into another dialysis bag, clamping the openings of the dialysis bags to prevent water leakage, adding the same weights to make the dialysis bags suspend or completely immerse in an external anaerobic ammonia-free water environment, placing at 20 to 35 DEG C for dialysis, measuring the concentration of the ammonia-nitrogen solution or the nitrite nitrogen solution in the external solutions of the dialysis bags every 1 hour, and fitting dialysis curves of two dialysis experiments with Origin8.0, wherein the adsorption amount of the ammonia nitrogen solution or the nitrite nitrogen solution by EPS is the difference between final external dialysis concentration fitted by a pure substrate and final external dialysis concentration fitted after adding of the EPS solution. The method has the advantages of easiness in operation and low detection cost for EPS adsorption of ammonia nitrogen or nitrite nitrogen.
Description
technical field [0001] The invention relates to a method for determining an extracellular polymer adsorption substrate, in particular to a method for determining an extracellular polymer adsorption substrate for anaerobic ammonium oxidizing bacteria fitted to a dialysis bag. Background technique [0002] As a mixed polymer such as protein and polysaccharide secreted or captured during the metabolic growth of microorganisms, EPS plays an important role in the growth of bacteria, especially for anammox bacteria. The EPS is an extracellular polymer, expressed in English as Extracellular Polymeric Substances , abbreviated as EPS. Because the granulation of anammox bacteria and the stability of granules not only rely on a certain concentration of substrate to ensure bacterial growth, but also rely on EPS to maintain bacterial granulation. At present, researchers have realized that EPS is secreted by anammox bacteria, and excessive EPS has adverse effects on the growth of anammox...
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IPC IPC(8): G01N30/00
CPCG01N30/00
Inventor 乔椋张雁秋李燕徐雯冒盛鑫吕振
Owner CHINA UNIV OF MINING & TECH