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Online solid phase extraction method for detecting diazepam and clozapine in human body serum and system thereof

A solid-phase extraction column and clozapine technology, which is applied in the field of online solid-phase extraction analysis system, can solve the problems of uneven elution of samples, high sample loss rate, high cost consumption, reduce sample loss, reduce interference, increase The effect of service life

Inactive Publication Date: 2018-10-30
苏州和合医学检验有限公司 +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, both the protein precipitation method and the liquid-liquid extraction method have the disadvantages of large reagent consumption and high sample loss rate.
The solid phase extraction method has problems such as high cost consumption and uneven sample elution

Method used

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  • Online solid phase extraction method for detecting diazepam and clozapine in human body serum and system thereof
  • Online solid phase extraction method for detecting diazepam and clozapine in human body serum and system thereof
  • Online solid phase extraction method for detecting diazepam and clozapine in human body serum and system thereof

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Experimental program
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Embodiment

[0032] Next, the present invention will be described in further detail through examples, but the present invention is not limited to these examples.

[0033] (1) Instruments and reagents

[0034]Ultimate 3000 dual ternary liquid chromatography system (Thermo scientific company), equipped with dual ternary gradient pumps, automatic sampler, column thermostat (with a six-way valve), diode array detector, chameleon chromatography management software; Centrifuge 5424 High-speed centrifuge (Eppendorf AG, USA); N-EVAP 111 nitrogen blowing instrument N-EVAP 111 (Organomation, USA); MSX (SG EA) electronic balance (sartorius, German); MIX-25P vortex mixer (MIUL AB ,U.S).

[0035] Haloperidol standard (National Institute for Food and Drug Control, batch number 100313-200301), diazepam standard (National Institute for Food and Drug Control, batch number 171225-200302), clozapine standard (European Pharmacopoeia Reference Standard, batch number C2460000), ammonium acetate (chromatograph...

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Abstract

The invention provides an online solid phase extraction method for detecting diazepam and clozapine in human body serum and a system thereof. The invention aims to provide an online slid phase extraction technology and high-efficiency liquid phase chromatography coupling method, wherein the concentrations of two drugs of diazepam and clozapine in the human body serum can be simultaneously measured. Through methodology validation, the online solid phase extraction method and the system thereof have advantages of high detection accuracy, high detecting rate, high extraction recovery rate and high repeatability. Furthermore the invention provides an online solid phase extraction analysis system for detecting the diazepam and the clozapine in the human body serum.

Description

technical field [0001] The invention discloses a combined application technology of on-line solid-phase extraction and high-performance liquid chromatography, and establishes an analysis method for simultaneously determining diazepam and clozapine in human serum. An online solid-phase extraction analysis system for the detection of diazepam and clozapine. Background technique [0002] Diazepam is a long-acting benzodiazepine drug with anti-anxiety, sedative-hypnotic, anti-convulsant, anti-epileptic and central muscle relaxation effects; clozapine is currently recognized as the most effective antipsychotic drug for the treatment of schizophrenia , It is widely used clinically to control nervous system diseases such as insomnia, depression, and epilepsy. Diazepam is used in combination with clozapine to treat schizophrenia. However, because the patient is mentally abnormal, it is easy to cause poisoning due to overdose, which endangers the health and life safety of the patie...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06G01N2030/062
Inventor 尚婵丁利贾继明王鹏田蕊邹皓月
Owner 苏州和合医学检验有限公司
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