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Application of Bcl-3 as marker for diagnosing early renal fibrosis

A technology of renal fibrosis and bcl-3, applied in the field of diagnosis, can solve the problems of physical trauma of patients, difficulty in popularization, and difficulty in ensuring the validity and accuracy of diagnosis.

Active Publication Date: 2018-11-02
SHANGHAI INST OF BIOLOGICAL SCI CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the clinical diagnosis of renal fibrosis is still based on renal histopathological biopsy, but as an invasive diagnostic method, it will cause a certain degree of physical trauma or even death to the patient, and it is difficult to dynamically and continuously observe the progress of the disease
Detecting the content of creatinine and urea in serum is also a commonly used clinical diagnostic method, but it is difficult to guarantee the effectiveness and accuracy of diagnosis, and the comprehensive detection and analysis of metabolic components in urine by nuclear magnetic resonance is too costly and difficult to popularize
In addition, most of the pathological phenomena that these detection methods can detect are accompanying phenomena caused by the decline of renal function, and it is difficult to detect the occurrence of the disease in the early stage of renal fibrosis

Method used

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  • Application of Bcl-3 as marker for diagnosing early renal fibrosis
  • Application of Bcl-3 as marker for diagnosing early renal fibrosis
  • Application of Bcl-3 as marker for diagnosing early renal fibrosis

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Embodiment approach

[0074] As an embodiment of the present invention, the reagent is an anti-Bcl-3 antibody; more specifically, it is a monoclonal antibody or a polyclonal antibody.

[0075] Antibodies of the present invention can be prepared by various techniques known to those skilled in the art. For example, purified antigen can be administered to animals such as rabbits, mice, rats, etc. to induce polyclonal antibody production. Various adjuvants can be used to enhance the immune response, including but not limited to Freund's adjuvant and the like.

[0076] Antibodies of the invention may also be monoclonal antibodies. Such monoclonal antibodies can be prepared using hybridoma technology.

[0077] The antibody can be used in immunochemical technique to detect the Bcl-3 level in the sample, so as to diagnose renal fibrosis, especially early renal fibrosis.

[0078] As another embodiment of the present invention, the reagent is a primer for specifically amplifying the Bcl-3 gene. As a more...

Embodiment 1

[0135] Embodiment 1, gene screening

[0136] Based on the data recorded in GSE79443, the present inventors analyzed the data of the whole transcriptome differential gene analysis of the renal fibrosis mouse model. In combination with the inventor's experience, during the analysis process, a strict screening scheme (P1.5 times) was used to screen out 1736 differential genes from about 40,000 genes, and some analysis results are as follows: figure 1 a.

[0137] Then, the inventors determined 37 differential genes related to NF-kB signaling pathway through pathway enrichment (https: / / david.ncifcrf.gov / ) method, such as figure 1 b. Analyzing the 37 differential genes, the inventors found that the mRNA of Bcl-3 was significantly high-expressed in the early stage of renal fibrosis, which has a very obvious advantage.

Embodiment 2

[0138] Example 2, Screening of Abnormally Expressed Genes

[0139] In the experiment, 6-8 week male C57-WT mice were selected for unilateral ureteral ligation on the left side, and sham operations were performed on mice in the control group, and the kidneys on both sides were removed 15 days after ligation. In the experiment, 4.0 surgical thread was used for ligation or free ligation.

[0140] Compared with the contralateral kidney, in the left kidney of mice after ureteral ligation for 15 days, a large amount of urine accumulated in the renal pelvis; The boundary between the cortex and medulla is unclear, and the degree of fibrosis is very obvious, such as figure 2 a.

[0141] The present inventors performed H&E and Sirius staining on the left and right kidneys of the sham-operated group and the left and right kidneys of mice ligated for 15 days, and observed the changes in the overall structure and morphology of the renal cortex after unilateral ureteral ligation. The st...

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Abstract

The invention relates to application of Bcl-3 as a marker for diagnosing early renal fibrosis. Compared with the conventional diagnostic marker for renal fibrosis, Bcl-3 has the advantage that Bcl-3 level in serum can reflect the occurrence of disease at an earlier stage of the renal fibrosis, so that the Bcl-3 is a good diagnostic marker.

Description

technical field [0001] The invention belongs to the field of diagnosis, and more specifically, the invention relates to the application of Bcl-3 as a marker for diagnosing early renal fibrosis. Background technique [0002] Chronic kidney disease (chronic kidney disease, CKD) usually leads to renal fibrosis, and eventually irreversibly develops into advanced renal failure. It is accompanied by some cardiovascular complications and increases the risk of death of patients. It is a worldwide problem. The incidence of chronic kidney disease in China remains high, and more than one million people die from chronic kidney disease and its complications every year. It is reported that the number of patients with chronic kidney disease will surge worldwide within 20 years from 2010 to 2030 nearly doubled. [0003] Chronic kidney injury is a progressive kidney disease mainly due to the accumulation of type I collagen and other extracellular matrix proteins leading to changes in kidney...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6883G01N33/68
CPCG01N33/6875C12Q1/6883G01N2800/7052C12Q2600/158
Inventor 张笑人陈然
Owner SHANGHAI INST OF BIOLOGICAL SCI CHINESE ACAD OF SCI