A miRNA combination for detecting brain metastasis of lung cancer and a kit containing the combination

Abstract

The invention discloses a miRNA combination for detecting brain metastasis of lung cancer and a kit containing the combination. The miRNA combination includes miR-10b-3p, miR-200c-3p, and miR-145-5p. The kit includes forward and reverse primers of the above miRNA combination, forward and reverse primers of external reference U6 RNA, cerebrospinal fluid RNA extraction system, reverse transcription reagent, PCR amplification reagent, cDNA mixed negative control substance and cDNA mixed positive control substance. The present invention adopts a group of miRNAs as new markers of brain metastases of lung cancer, which can improve the diagnostic low efficiency including sensitivity and specificity, which is difficult for a single marker, and can also avoid missed detection and misjudgment in single detection and traditional detection methods. The present invention provides an auxiliary screening kit using the above-mentioned miRNA combination for lung cancer prognosis and brain metastases, which is not only convenient for detection, but also accurate in quantification, and greatly improves the sensitivity and specificity of disease detection. Clinical lung cancer brain metastases risk assessment has an auxiliary role.

Description

technical field [0001] The invention belongs to the technical field of medical molecular biology, and in particular relates to a miRNA combination for detecting brain metastasis of lung cancer and a kit containing the combination. Background technique [0002] In recent years, with the increase in the incidence of lung cancer and the continuous development of diagnosis and treatment technology, the survival period of patients has been prolonged, and the occurrence and diagnosis rate of lung cancer brain metastases have also increased year by year. The incidence of brain metastases from lung cancer is significantly higher than that of melanoma, breast cancer, kidney cancer and colorectal cancer. 20%-65% of lung cancer patients will develop brain metastases during the course of the disease, which is the most common type of brain metastases. Adenocarcinoma has a higher probability of brain metastases, accounting for 40%-70% of intracranial metastases. It is extremely occult, an...

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Problems solved by technology

Clinical data also confirm that there are too many influencing factors to rely on a certain marker alone to diagnose a disease, so it is not as valuable as combined diagnosis

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