Algae red tide and toxicity detection method based on three-dimensional fluorescence spectroscopy

Abstract

The invention discloses a method for detecting red tide and toxicity of seawater algae based on three-dimensional fluorescence spectrum, comprising the following steps: irradiating seawater to be detected with monochromatic ultraviolet light; collecting reflected fluorescence of seawater; The three-dimensional fluorescence spectrum of the seawater is converted into a three-dimensional fluorescence spectrum in the form of electrical signals by CCD, and the original three-dimensional fluorescence spectrum data of seawater is obtained; the original three-dimensional fluorescence spectrum data is represented in parallel by a quaternion matrix; the extraction of spectral features based on quaternion principal component analysis; A nonlinear mapping model was established for the identification of hemolytic activity of red tide algae, and the extracted spectral features were brought into the model. The detection method of the invention is based on a spectral method without adding chemical reagents; the equipment used in the detection method is small and highly integrated, and can realize uninterrupted in-situ online measurement; and the detection precision is high.

Description

technical field [0001] The invention belongs to the technical field of photoelectric detection, and relates to a method for detecting seawater algae red tide and toxicity based on three-dimensional fluorescence spectrum. Background technique [0002] Harmful algal bloom (HAB), also known as red tide, is a harmful ecological phenomenon in which some phytoplankton, protozoa or bacteria explosively multiply or highly gather in seawater under certain environmental conditions, causing water discoloration. Red tides can sometimes cause great harm to fish and other aquatic animals. This is caused by red tide plankton clogging fish gills, causing mechanical obstacles, and decomposing after death, rapidly consuming oxygen, insufficient oxygen in the water, and secreting harmful substances. Ichthyotoxic red tide can produce a variety of toxic substances, causing a large number of fish and shellfish to die in a short period of time. Fish-toxic red tide algae have been widely distribut...

AI Technical Summary

Problems solved by technology

Because the absorption spectrum of phytoplankton is easily affected by yellow substances and photoprotective pigments, the further development of pigment spectroscopy is limited.

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